hTERT基因多態(tài)性與宮頸癌前病變的關(guān)聯(lián)性研究
[Abstract]:[Objective] To study the relationship between the polymorphism of human telomerase reverse transcriptase (hTERT) gene and the development of precancerous lesion of cervical cancer. [Methods] A total of 200 healthy women with high-risk HPV infection and no cervical lesions were selected from January 2015 to September 2016 at the Third Affiliated Hospital of Kunming Medical University. In the first group,300 patients with premalignant cervical cancer were diagnosed as experimental group. and collecting the DNA of the specimen from the peripheral blood of the cervical tissue and the control group, collecting the DNA of the specimen, using the TaqMan probe gene typing method, performing gene sequencing on the four SNP loci rs2736122 (G A), rs2853676 (CT), rs2853677 (AG), rs2075786 (AG) of the hTERT gene, and constructing a haplotype, The frequency and difference of each SNP locus allele, genotype and haplotype between the experimental group and the control group and at different levels of the cervical squamous intraepithelial lesions were calculated. [Results] 1. The results of the Hardy-Weinberg equilibrium test showed that the genotype distribution of the four SNP sites of the hTERT gene in the experimental group and the control group was all in accordance with the Hardy-Weinberg equilibrium (P0.05). The genotypes of rs2736122 (GA), rs2853676 (CT), rs2853677 (AG) and rs2075786 (AG) in the control group were not statistically significant. The results of linkage disequilibrium analysis showed that in 4 groups, there was a strong linkage disequilibrium between rs2075786 (AG) and rs2736122 (GA), and D '0.8; the correlation between the other groups was not obvious, and D' 0.4.4. The haplotype of the hTERT gene rs2075786/ rs2736122 was constructed according to the results of the linkage disequilibrium analysis. The results showed that the single-fold frequency distribution of A-G, G-A and G-G was between the pre-cervical lesion and the healthy control group. The results showed that rs2736122 (GA), rs2853676 (CT), rs2736122 (GA), rs2853676 (CT), rs2075786 (ag) in the low-grade cervical squamous intraepithelial lesion group and the high-level cervical squamous intraepithelial lesion group, there was no significant difference in the allele and genotype frequency difference, p0.05 rs2853677 (ag) site, There was no significant difference in allele frequency among the lower-grade cervical squamous intraepithelial lesion group and the high-level cervical squamous intraepithelial lesion group, and the P value was 0.330; however, the frequency difference between the two groups was statistically significant and the P value was 0.012. The results of the genetic analysis showed that the gene of the hTERT gene rs2853677 (AG), the superdominant genetic pattern may be the best genetic model of the site, and in the superdominant genetic model, the genotype A/ A + G/ G was used as the reference. The P =:2-104, OR = 2.83,95% CI = 1.61-4.99 in the low-grade cervical squamous intraepithelial lesion group and the high-grade cervical squamous intraepithelial lesion group, the difference was statistically significant, P0.05, The AG genotype has increased the risk of further development of low-grade cervical squamous intraepithelial lesions to high-level cervical squamous intraepithelial lesions. [Conclusion] 1. The polymorphism of rs2736122 (GA), rs2853676 (C T) and rs2075786 (AG) of the hTERT gene is not related to the risk of the development of cervical cancer. 2. The rs2853677 (AG) polymorphism of the hTERT gene is not related to the risk of the premalignant lesion of cervical cancer, but there is a correlation with the development of the pre-cervical cancer. A/ G genotype may increase the risk of high-level cervical squamous intraepithelial lesions.3. The haplotype distribution of the hTERT gene rs2075786/ rs2736122 has no significant correlation with the development of pre-cervical lesions.
【學(xué)位授予單位】:昆明醫(yī)科大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2017
【分類號】:R737.33
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