【摘要】：目的:建立穩(wěn)定的宮腔粘連細(xì)胞模型,檢測叉頭框F2(Fox F2)表達(dá)。方法:原代培養(yǎng)人子宮內(nèi)膜基質(zhì)細(xì)胞(HESCs),免疫熒光法進(jìn)行細(xì)胞鑒定。用0、2.5、5和10ng/ml轉(zhuǎn)化生長因子β1(TGF-β1)作用于HESCs 48h,10ng/ml TGF-β1作用于HESCs 12、24、48h和72h,PCR及Western blot法檢測α-平滑肌肌動蛋白(α-SMA)、膠原I(COLI)及Fox F2 m RNA和蛋白表達(dá)。結(jié)果:與0ng/ml組比較,2.5、5ng/ml和10ng/ml組α-SMA、COLI及Fox F2的m RNA和蛋白表達(dá)均逐漸增加。隨著10ng/ml TGF-β1作用時間的延長,與12h組相比,24、48和72h組α-SMA、COLI和Fox F2的m RNA和蛋白表達(dá)逐漸增加。結(jié)論:TGF-β1可誘導(dǎo)HESCs發(fā)生纖維化改變,建立宮腔粘連細(xì)胞模型。隨著TGF-β1作用時間的延長,作用濃度的增加,Fox F2在宮腔粘連中表達(dá)增加。
[Abstract]:Aim: to establish a stable model of uterine cavity adhesion cells and detect the expression of Fox F2. Methods: primary cultured human endometrial stromal cells were identified by (HESCs), immunofluorescence assay. The HESCs was treated with 0, 2.5, 5 and 10ng/ml transforming growth factor 尾 1 (TGF- 尾 1) for 48 h, and 10 ng / ml TGF- 尾 1 was treated with HESCs 12, 24, 48 h and 72 h. The 偽-SMA), was detected by Western blot. The expression of collagen I (COLI) and Fox F 2m RNA and protein were observed. Results: compared with 0ng/ml group, the mRNA and protein expression of 偽-SMA,COLI and Fox F2 in 2.5 ng / ml, 5 ng / ml and 10ng/ml groups increased gradually. Compared with 12h group, the expression of 偽-SMA,COLI and Fox F2 mRNA and protein in 24, 48 and 72 h groups increased gradually with the prolongation of 10ng/ml TGF- 尾 1 treatment time. Conclusion: TGF- 尾 1 can induce fibrosis changes of HESCs and establish uterine adhesion cell model. With the prolongation of TGF- 尾 1 treatment time and the increase of concentration, the expression of Fox F2 in uterine cavity adhesion increased.
【作者單位】： 南方醫(yī)科大學(xué)珠江醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學(xué)基金資助(No:81270658)
【分類號】：R711.74

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本文編號：2459016