發(fā)布時(shí)間：2019-02-27 21:10

【摘要】：為了觀察產(chǎn)前束縛應(yīng)激對子代大鼠空間學(xué)習(xí)記憶能力、海馬神經(jīng)干細(xì)胞增殖及巢蛋白表達(dá)的影響,將體重240~260 g的Sprague-Dawley雌性母鼠12只隨機(jī)分成2組,對照組于孕期不做任何處理,束縛應(yīng)激組于孕14~20 d時(shí)給予束縛應(yīng)激,3次/天,45 min/次。取1月齡子代大鼠進(jìn)行實(shí)驗(yàn)研究。Morris水迷宮定位航行實(shí)驗(yàn)結(jié)果顯示,應(yīng)激組子代與對照組相比,到達(dá)平臺(tái)的潛伏期延長(P0.05),而在空間探索實(shí)驗(yàn)中,應(yīng)激組子代在原平臺(tái)象限停留時(shí)間與對照組相比無顯著差異。免疫組織化學(xué)結(jié)果顯示,應(yīng)激組雌性子代海馬巢蛋白(nestin)和BrdU陽性細(xì)胞表達(dá)均較對照組顯著增加(P0.05),而雄性子代海馬nestin和BrdU陽性細(xì)胞表達(dá)與對照組相比無顯著性差異(P0.05)。以上結(jié)果提示,產(chǎn)前束縛應(yīng)激可引起雌性子代大鼠海馬神經(jīng)干細(xì)胞數(shù)量增加以及增殖能力增強(qiáng),可能與機(jī)體對產(chǎn)前應(yīng)激所致腦損傷的代償性反應(yīng)相關(guān)。
[Abstract]:In order to observe the effects of prenatal restraint stress on spatial learning and memory ability, proliferation of hippocampal neural stem cells (NSCs) and expression of nestin, 12 female Sprague-Dawley female rats weighing 240? 260 g were randomly divided into two groups. In the control group, no treatment was given during pregnancy, while in the restraint stress group, restraint stress was given on the 14th day of gestation, 3 times a day, and 45 min/ times at the 20th day of gestation. Morris water maze navigation experiment showed that the latency of the stress group was longer than that of the control group (P0.05), while in the space exploration experiment, the latency of the stress group was longer than that of the control group (P0.05). There was no significant difference in the retention time of the offspring of the stress group between the original platform quadrant and the control group. Immunohistochemical results showed that the expression of nestin (nestin) and BrdU in hippocampus of female offspring in stress group was significantly higher than that in control group (P0.05). There was no significant difference in the expression of nestin and BrdU positive cells between male offspring and control group (P0.05). These results suggest that prenatal restraint stress can increase the number of neural stem cells and enhance the proliferation of neural stem cells in hippocampus of female offspring, which may be related to the compensatory response of the organism to brain injury induced by prenatal stress.
【作者單位】： 西安醫(yī)學(xué)院第一附屬醫(yī)院婦科;西安醫(yī)學(xué)院第一附屬醫(yī)院藥劑科;西安交通大學(xué)第二附屬醫(yī)院婦產(chǎn)科;西安交通大學(xué)第一附屬醫(yī)院兒科;
【基金】：supported by the Science and Technology Plan Project of the Department of Education of Shaanxi Province,China(No.16JK1643)
【分類號(hào)】：R714

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