【摘要】：目的:探討mi R-195及IKBKB蛋白在子癇前期患者胎盤組織中的表達(dá)及意義。方法:分別選擇2015年08月份到2016年06月份期間在山西醫(yī)科大第二附屬醫(yī)院的產(chǎn)科的住院的并生產(chǎn)的PE患者各30例,分別采集相應(yīng)的臨床資料以及收集胎盤組織;應(yīng)用RT-qPCR法檢測(cè)胎盤中的miR-195的相對(duì)表達(dá)水平;應(yīng)用免疫組織化學(xué)法以及Western-blot法檢測(cè)胎盤組織IKBKB蛋白的表達(dá);使用SPSS 17.0軟件進(jìn)行統(tǒng)計(jì)學(xué)處理。結(jié)果:(1)研究組與正常對(duì)照組在年齡(t=1.86)和體重指數(shù)(t=0.92)之間無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。兩組分娩時(shí)的孕周[(32.8±0.77)vs(39.02±0.69),t=6.24,P(27)0.01]、收縮壓[(180.9±9.2)vs(118.6±3.27),t=7.38,P(27)0.01]、舒張壓[(118.7±6.02)vs(69.2±2.18),t=8.82,P(27)0.01]、新生兒出生體重[(1737±201.3)vs(2870±193.2),t=3.84,P(27)0.01]之間差異有統(tǒng)計(jì)學(xué)意義(P0.01);(2)子癇前期患者miR-195在子癇前期研究組胎盤中的相對(duì)表達(dá)量為正常孕婦組的(0.36±0.20)倍;(3)生物信息學(xué)分析顯示IKBKB可能為miR-195的靶基因;(4)子癇前期研究組胎盤滋養(yǎng)細(xì)胞中IKBKB蛋白的表達(dá)(OD:0.08±0.01)顯著低于正常妊娠對(duì)照組(OD:0.13±0.01),差異有統(tǒng)計(jì)學(xué)意義(t=11.63,P0.001);(5)Western-blot檢測(cè)IKBKB蛋白顯示子癇前期研究組胎盤滋養(yǎng)細(xì)胞中IKBKB蛋白的相對(duì)表達(dá)量(0.08±0.01)顯著低于正常妊娠對(duì)照組(0.13±0.01),差異有統(tǒng)計(jì)學(xué)意義(t=13.94,P0.001);(6)相關(guān)性分析表明本實(shí)驗(yàn)中IKBKB與miR-195無(wú)相關(guān)性(r=0.1227,P=0.5503)。結(jié)論:子癇前期患者胎盤組織中miR-195與IKBKB蛋白表達(dá)水平下調(diào),提示miR-195及IKBKB蛋白可能通過(guò)調(diào)節(jié)滋養(yǎng)細(xì)胞凋亡參與子癇前期的發(fā)生和發(fā)展。
[Abstract]:Objective: to investigate the expression and significance of mi R 195 and IKBKB protein in placenta of preeclampsia. Methods: from August 2015 to June 2016, 30 cases of PE patients who were hospitalized and delivered in the second affiliated Hospital of Shanxi Medical University were selected, and the corresponding clinical data and placenta tissue were collected. The relative expression of miR-195 in placenta was detected by RT-qPCR method, the expression of IKBKB protein in placenta was detected by immunohistochemical method and Western-blot method, and the expression of IKBKB protein was analyzed by SPSS 17.0 software. Results: (1) there was no significant difference in age (t = 1.86) and body mass index (t = 0.92) between the study group and the normal control group (P0.05). The gestational weeks at delivery in both groups [(32.8 鹵0.77) vs (, 39.02 鹵0.69), 6.24) vs (P (27) 0.01], SBP [(180.9 鹵9.2) vs (118.6 鹵3.27), t 7.38) vs (P (27) 0.01]. Diastolic blood pressure (DBP) [(118.7 鹵6.02) vs (, 69.2 鹵2.18), t = 8.82) vs (, P (27) 0.01], neonatal birth weight [(1737 鹵201.3) vs (, 2870 鹵193.2), t = 3.84], P (27) 0.01] the difference was statistically significant (P0.01). (2) the relative expression of miR-195 in placenta of preeclampsia study group was 0.36 鹵0.20 times higher than that of normal pregnant women, (3) bioinformatics analysis showed that IKBKB might be the target gene of miR-195. (4) the expression of IKBKB protein in placental trophoblast of preeclampsia study group (OD:0.08 鹵0. 01) was significantly lower than that of normal pregnancy group (OD:0.13 鹵0. 01). (5) the relative expression of IKBKB protein in placental trophoblastic cells of preeclampsia study group (0.08 鹵0.01) was significantly lower than that of normal pregnancy group (0.13 鹵0.01), and the difference was statistically significant (t _ (13) 94). (P0.001); (6) correlation analysis showed that there was no correlation between IKBKB and miR-195 in this experiment (r = 0.1227). Conclusion: the expression of miR-195 and IKBKB proteins in placenta of preeclampsia patients is down-regulated, suggesting that miR-195 and IKBKB proteins may be involved in the occurrence and development of preeclampsia by regulating the apoptosis of trophoblast.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R714.244

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