【摘要】：目的： 探討高壓氧暴露對(duì)卵巢早衰大鼠骨代謝的影響。 方法： 將40只SPF級(jí)Wistar大鼠隨機(jī)分為4組：空白組、造�？瞻捉M、雌孕激素替代(hormone replace therapy, HRT)組、高壓氧(hyperbaric oxygen, HBO)組。除空白組以外,均給予雷公藤多甙片溶液灌胃,以建立卵巢早衰模型。然后,HRT組給予雌孕激素灌胃治療,HBO組給予高壓氧治療及等量生理鹽水灌胃,空白組及造�？瞻捉M給予等量生理鹽水灌胃。觀察血清骨堿性磷酸酶(BALP)、抗酒石酸酸性磷酸酶(TRACP)、抗苗勒管激素(AMH)在不同組群變化。 結(jié)果： 1.與空白組相比較,造模空白組BLAP、AMH均顯著降低；TRACP則顯著升高,差異均有顯著統(tǒng)計(jì)學(xué)意義。2.干預(yù)治療后,HRT組的TRACP顯著降低,差異有顯著意義；BALP、AMH與造模空白組相比均升高,BALP升高有統(tǒng)計(jì)學(xué)意義,而AMH無(wú)統(tǒng)計(jì)學(xué)意義。3.干預(yù)治療后,HBO組的BALP、AMH與造�？瞻捉M相比均升高；TRACP顯著降低,差異均有統(tǒng)計(jì)學(xué)意義。4.與HRT組相比,HBO組AMH較高,差異有統(tǒng)計(jì)學(xué)意義；BALP較低,TRACP較高,但差異均無(wú)統(tǒng)計(jì)學(xué)意義。 結(jié)論： 高壓氧暴露能夠改善卵巢功能,影響卵巢早衰大鼠的骨代謝,對(duì)促進(jìn)骨形成的BALP有增強(qiáng)作用,對(duì)促進(jìn)骨吸收的TRACP有抑制作用。
[Abstract]:Objective: to investigate the effect of hyperbaric oxygen exposure on bone metabolism in premature ovarian failure rats. Methods: forty SPF grade Wistar rats were randomly divided into 4 groups: blank group, model blank group, estrogen and progesterone replacement (hormone replace therapy, HRT) group, hyperbaric oxygen (hyperbaric oxygen, HBO) group. The model of premature ovarian failure was established by oral administration of Tripterygium wilfordii polyglycoside solution except blank group. Then, the HRT group was treated with estrogen and progesterone, the HBO group was treated with hyperbaric oxygen and the same amount of normal saline, and the blank group and the model blank group were given the same amount of normal saline. The changes of serum bone alkaline phosphatase (BALP),) and tartrate acid phosphatase (TRACP),) against Muller tube hormone (AMH) in different groups were observed. Results: 1. Compared with the blank group, the BLAP,AMH of the model blank group decreased significantly, while the TRACP increased significantly, the difference was significant. 2. 2. After intervention, the TRACP of HRT group was significantly lower than that of model blank group, BALP,AMH was higher than that of model blank group, BALP increased significantly, but AMH had no statistical significance. After intervention, BALP,AMH in HBO group was significantly higher than that in model blank group, and TRACP was significantly lower than that in model blank group. 4. 4. Compared with HRT group, the AMH of HBO group was higher than that of HRT group, the difference was statistically significant, BALP was lower, TRACP was higher, but the difference was not statistically significant. Conclusion: hyperbaric oxygen exposure can improve ovarian function, affect bone metabolism in premature ovarian failure rats, enhance bone formation of BALP and inhibit bone resorption of TRACP.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R711.75

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