【摘要】：目的 :探討腸凝集素1(intelectin-1,ITLN-1)穩(wěn)定表達(dá)對人子宮內(nèi)膜腺癌Ishikawa細(xì)胞裸鼠移植瘤生長的影響。方法 :16只裸鼠隨機(jī)分為2組,分別皮下接種感染慢病毒GV358(攜帶有ITLN-1基因的慢病毒)的Ishikawa細(xì)胞(ITLN-1組)和感染空載體慢病毒(僅含有空載體的慢病毒)的Ishikawa細(xì)胞(對照組),建立裸鼠人子宮內(nèi)膜癌Ishikawa細(xì)胞移植瘤模型。測量小鼠皮下腫瘤體積,繪制腫瘤生長曲線,剝離腫瘤后稱取腫瘤的質(zhì)量,計(jì)算腫瘤抑制率;腫瘤組織切片行HE染色觀察病理學(xué)形態(tài);免疫組織化學(xué)法檢測腫瘤組織中p53、環(huán)氧合酶2(cyclooxygenase-2,Cox-2)和Bcl-2蛋白的表達(dá)情況。結(jié)果 :與對照組相比,ITLN-1組小鼠移植瘤的生長受到明顯抑制(P0.01);免疫組織化學(xué)結(jié)果顯示,ITLN-1組中野生型p53蛋白的表達(dá)水平明顯上調(diào)(P0.01),而Cox-2和Bcl-2蛋白的表達(dá)水平明顯下調(diào)(P值均0.01)。結(jié)論 :ITLN-1過表達(dá)能抑制裸鼠人子宮內(nèi)膜癌移植瘤的生長,發(fā)揮抗腫瘤作用。
[Abstract]:Aim: to investigate the effect of stable expression of lectin 1 (intelectin-1,ITLN-1) on the growth of human endometrial adenocarcinoma Ishikawa cells in nude mice. Methods: sixteen nude mice were randomly divided into two groups. Ishikawa cells infected with lentivirus GV358 (lentivirus carrying ITLN-1 gene) (ITLN-1 group) and Ishikawa cells infected with empty vector lentivirus (lentivirus only) were inoculated subcutaneously (control group). Human endometrial carcinoma (Ishikawa) transplanted tumor model was established in nude mice. The tumor volume was measured, the tumor growth curve was drawn, the tumor mass was removed after the tumor was stripped, the tumor inhibition rate was calculated, and the pathological morphology was observed by HE staining. The expression of p53, cyclooxygenase 2 (cyclooxygenase-2,Cox-2) and Bcl-2 protein were detected by immunohistochemistry. Results: compared with the control group, the growth of transplanted tumor in ITLN-1 group was significantly inhibited (P0.01). Immunohistochemical results showed that the expression of wild-type p53 protein was up-regulated in ITLN-1 group (P0.01), while the expression of Cox-2 and Bcl-2 protein was down-regulated (P0.01). Conclusion: overexpression of ITLN-1 can inhibit the growth of human endometrial carcinoma xenografts in nude mice and play an anti-tumor role.
【作者單位】： 河北醫(yī)科大學(xué)第四醫(yī)院婦科;
【基金】：河北省衛(wèi)生廳科研基金項(xiàng)目(編號:20130233)~~
【分類號】：R737.33

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