【摘要】：目的 :探討腸凝集素1(intelectin-1,ITLN-1)穩(wěn)定表達(dá)對人子宮內(nèi)膜腺癌Ishikawa細(xì)胞裸鼠移植瘤生長的影響。方法 :16只裸鼠隨機分為2組,分別皮下接種感染慢病毒GV358(攜帶有ITLN-1基因的慢病毒)的Ishikawa細(xì)胞(ITLN-1組)和感染空載體慢病毒(僅含有空載體的慢病毒)的Ishikawa細(xì)胞(對照組),建立裸鼠人子宮內(nèi)膜癌Ishikawa細(xì)胞移植瘤模型。測量小鼠皮下腫瘤體積,繪制腫瘤生長曲線,剝離腫瘤后稱取腫瘤的質(zhì)量,計算腫瘤抑制率;腫瘤組織切片行HE染色觀察病理學(xué)形態(tài);免疫組織化學(xué)法檢測腫瘤組織中p53、環(huán)氧合酶2(cyclooxygenase-2,Cox-2)和Bcl-2蛋白的表達(dá)情況。結(jié)果 :與對照組相比,ITLN-1組小鼠移植瘤的生長受到明顯抑制(P0.01);免疫組織化學(xué)結(jié)果顯示,ITLN-1組中野生型p53蛋白的表達(dá)水平明顯上調(diào)(P0.01),而Cox-2和Bcl-2蛋白的表達(dá)水平明顯下調(diào)(P值均0.01)。結(jié)論 :ITLN-1過表達(dá)能抑制裸鼠人子宮內(nèi)膜癌移植瘤的生長,發(fā)揮抗腫瘤作用。
[Abstract]:Aim: to investigate the effect of stable expression of lectin 1 (intelectin-1,ITLN-1) on the growth of human endometrial adenocarcinoma Ishikawa cells in nude mice. Methods: sixteen nude mice were randomly divided into two groups. Ishikawa cells infected with lentivirus GV358 (lentivirus carrying ITLN-1 gene) (ITLN-1 group) and Ishikawa cells infected with empty vector lentivirus (lentivirus only) were inoculated subcutaneously (control group). Human endometrial carcinoma (Ishikawa) transplanted tumor model was established in nude mice. The tumor volume was measured, the tumor growth curve was drawn, the tumor mass was removed after the tumor was stripped, the tumor inhibition rate was calculated, and the pathological morphology was observed by HE staining. The expression of p53, cyclooxygenase 2 (cyclooxygenase-2,Cox-2) and Bcl-2 protein were detected by immunohistochemistry. Results: compared with the control group, the growth of transplanted tumor in ITLN-1 group was significantly inhibited (P0.01). Immunohistochemical results showed that the expression of wild-type p53 protein was up-regulated in ITLN-1 group (P0.01), while the expression of Cox-2 and Bcl-2 protein was down-regulated (P0.01). Conclusion: overexpression of ITLN-1 can inhibit the growth of human endometrial carcinoma xenografts in nude mice and play an anti-tumor role.
【作者單位】： 河北醫(yī)科大學(xué)第四醫(yī)院婦科;
【基金】：河北省衛(wèi)生廳科研基金項目(編號:20130233)~~
【分類號】：R737.33

【相似文獻(xiàn)】

相關(guān)期刊論文 前8條

1 余思云;李運延;胡國華;;紫草素誘導(dǎo)子宮內(nèi)膜癌Ishikawa細(xì)胞凋亡及其機制的研究[J];遼寧中醫(yī)雜志;2013年09期

2 金陽;張玉泉;;西羅莫司對人子宮內(nèi)膜癌Ishikawa細(xì)胞增殖的影響及其機制[J];實用臨床醫(yī)藥雜志;2011年21期

3 管一春;楊雪峰;王興玲;孫麗君;張彩霞;郝大勇;王雪梅;;不同濃度胰島素作用的Ishikawa細(xì)胞中白血病抑制因子(LIF)的表達(dá)研究[J];軍醫(yī)進修學(xué)院學(xué)報;2011年09期

4 李軍;薛曉鷗;劉小麗;方杰;謝偉;;小檗堿對與腫瘤相關(guān)巨噬細(xì)胞共培養(yǎng)的人子宮內(nèi)膜癌Ishikawa細(xì)胞株的作用[J];北京中醫(yī)藥大學(xué)學(xué)報;2011年07期

5 刁振宇;盧悟廣;曹鵬;胡云龍;周星;薛平平;沈莉;孫海翔;;EGFR納米抗體抑制雌激素依賴的人子宮內(nèi)膜癌Ishikawa細(xì)胞的增殖與遷移[J];藥學(xué)學(xué)報;2012年10期

6 鄭靜;孫笑;王晶;王麗華;王玉東;;三氟拉嗪對人子宮內(nèi)膜癌Ishikawa細(xì)胞的抑制作用[J];上海交通大學(xué)學(xué)報(醫(yī)學(xué)版);2014年05期

7 張勤;黃娟;李丹青;何躍東;;蛋白酶體抑制劑MG132對人子宮內(nèi)膜癌HEC-1B和Ishikawa細(xì)胞增殖、凋亡和周期的影響[J];四川大學(xué)學(xué)報(醫(yī)學(xué)版);2012年06期

8 ;[J];;年期

相關(guān)碩士學(xué)位論文 前2條

1 鄭靜;三氟拉嗪對人子宮內(nèi)膜癌Ishikawa細(xì)胞的抑制作用[D];上海交通大學(xué);2015年

2 賈利剛;大蒜素單獨及與順鉑聯(lián)用對人子宮內(nèi)膜癌Ishikawa細(xì)胞的影響[D];河北醫(yī)科大學(xué);2006年

，

本文編號：2402237