【摘要】：目的:基于氣相色譜-質(zhì)譜聯(lián)用(GC-MS)技術(shù)研究復(fù)發(fā)性流產(chǎn)(RSA)的發(fā)病機(jī)制及可能的代謝標(biāo)記物,為干預(yù)和治療提供理論依據(jù)。方法:選擇20例健康早孕孕婦(正常對(duì)照組)和20例RSA早孕孕婦(RSA組)為研究對(duì)象,用GC-MS方法對(duì)兩組的尿液進(jìn)行代謝輪廓分析,比較兩組尿液代謝組分的差異。結(jié)果:兩組的總離子流圖顯示:有一些譜峰信號(hào)在RSA組和正常對(duì)照組之間存在著明顯的差異。采用PCA和PLS-DA對(duì)兩組尿樣代謝輪廓比較發(fā)現(xiàn):兩組的聚類(lèi)基本完全分開(kāi),但大部分在三維空間的同一區(qū)域。采用PLS-DA方法建模:VIP值1的差異尿液代謝物共69個(gè),匹配度80%的差異尿液代謝物共25個(gè)。結(jié)論:RSA孕婦和健康早孕孕婦的尿液代謝輪廓有明顯的差異,這可能是RSA的發(fā)病機(jī)制所在;25個(gè)差異物質(zhì)可能是RSA的代謝標(biāo)記物。
[Abstract]:Objective: to study the pathogenesis and possible metabolic markers of recurrent abortion (RSA) by gas chromatography-mass spectrometry (GC-MS) in order to provide theoretical basis for intervention and treatment. Methods: 20 healthy early pregnant women (normal control group) and 20 RSA early pregnant women (RSA group) were selected as study objects. The metabolic profile of urine in the two groups was analyzed by GC-MS method, and the differences of urine metabolic components between the two groups were compared. Results: the total ion flow patterns of the two groups showed that there were significant differences between the RSA group and the normal control group. PCA and PLS-DA were used to compare the metabolic profiles of the two groups of urine samples. It was found that the clustering of the two groups was completely separate, but most of them were in the same area in three dimensional space. PLS-DA method was used to model: 69 different urine metabolites with VIP value 1 and 25 different urine metabolites with matching degree of 80%. Conclusion: there are significant differences in the profile of urine metabolism between RSA pregnant women and healthy early pregnant women, which may be the pathogenesis of RSA, and 25 differential substances may be the metabolic markers of RSA.
【作者單位】： 湖南省婦幼保健院;
【分類(lèi)號(hào)】：R714.21

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