發(fā)布時間：2018-11-17 09:23

【摘要】：目的:通過將白細胞介素37(IL-37)基因轉(zhuǎn)染到宮頸癌HeLa細胞中,探討IL-37對宮頸癌細胞的殺傷作用及其對宮頸癌細胞化療敏感性的增強作用。方法:將重組pIRES2-EGFP/IL-37質(zhì)粒(IL-37組)和pIRES2-EGFP質(zhì)粒(NC組)分別轉(zhuǎn)染到HeLa細胞。Q-PCR和Western blotting法檢測IL-37基因和蛋白表達水平;CCK8法檢測NC組、IL-37組、順鉑(DDP)組及IL-37+DDP組細胞活性,計算細胞抑制率;RT-PCR法檢測信號轉(zhuǎn)導和轉(zhuǎn)錄激活因子3(STAT3)及細胞周期蛋白D1(Cyclin D1)mRNA表達水平。結(jié)果:與NC組比較,IL-37組IL-37mRNA和蛋白表達水平明顯升高(P0.01)。給藥后24~72h,5~15mg·L-1 DDP組HeLa細胞活性受到明顯抑制(P0.01);與DDP組比較,IL-37+DDP組HeLa細胞抑制率在處理后48h內(nèi)明顯升高(P0.05);與IL-37組比較,IL-37+DDP組HeLa細胞抑制率在處理后96h內(nèi)均有升高(P0.05)。與NC組比較,IL-37組STAT3和Cyclin D1mRNA表達水平明顯下降(P0.01)。結(jié)論:IL-37高表達可抑制宮頸癌細胞的增殖,并能增強DDP對宮頸癌細胞的放療效果,這種效應的發(fā)揮可能與IL-37使STAT3和Cyclin D1表達下調(diào)有關(guān)。
[Abstract]:Aim: to investigate the cytotoxicity and chemosensitivity of IL-37 to cervical cancer HeLa cells by transfection of interleukin 37 (IL-37) gene into cervical cancer HeLa cells. Methods: the recombinant pIRES2-EGFP/IL-37 plasmid (IL-37 group) and pIRES2-EGFP plasmid (NC group) were transfected into HeLa cells respectively. The expression of IL-37 gene and protein were detected by Q-PCR and Western blotting. The cell activity of NC group, IL-37 group, cisplatin (DDP) group and IL-37 DDP group were detected by CCK8, and the expression of signal transduction and transcription activator 3 (STAT3) and cyclin D1 (Cyclin D1) mRNA were detected by RT-PCR method. Results: the expression of IL-37mRNA and protein in IL-37 group was significantly higher than that in NC group (P0.01). The activity of HeLa cells in DDP group was significantly inhibited (P0.01), and the inhibition rate of HeLa cells in IL-37 DDP group was significantly higher than that in DDP group (P0.05). Compared with IL-37 group, the inhibition rate of HeLa cells in IL-37 DDP group was increased within 96 h after treatment (P0.05). Compared with NC group, the expression of STAT3 and Cyclin D1mRNA in IL-37 group decreased significantly (P0.01). Conclusion: the high expression of IL-37 can inhibit the proliferation of cervical cancer cells and enhance the radiotherapeutic effect of DDP on cervical cancer cells. This effect may be related to the down-regulation of STAT3 and Cyclin D1 expression induced by IL-37.
【作者單位】： 廣東醫(yī)科大學基礎(chǔ)醫(yī)學院組織學與胚胎學教研室;
【基金】：國家自然科學基金資助課題(81302244) 廣東省科技廳科技發(fā)展計劃項目資助課題(2016A020215147,2013B021800062) 廣東省衛(wèi)計委醫(yī)學科研基金項目資助課題(A2016208,B2013293) 廣東省東莞市科技局社會發(fā)展重點基金項目資助課題(2013108101051) 廣東醫(yī)科大學科研基金資助課題(M2016028) 廣東省湛江市科技局科技計劃項目資助課題(2013B01092)
【分類號】：R737.33

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