【摘要】：目的:通過將白細(xì)胞介素37(IL-37)基因轉(zhuǎn)染到宮頸癌HeLa細(xì)胞中,探討IL-37對(duì)宮頸癌細(xì)胞的殺傷作用及其對(duì)宮頸癌細(xì)胞化療敏感性的增強(qiáng)作用。方法:將重組pIRES2-EGFP/IL-37質(zhì)粒(IL-37組)和pIRES2-EGFP質(zhì)粒(NC組)分別轉(zhuǎn)染到HeLa細(xì)胞。Q-PCR和Western blotting法檢測IL-37基因和蛋白表達(dá)水平;CCK8法檢測NC組、IL-37組、順鉑(DDP)組及IL-37+DDP組細(xì)胞活性,計(jì)算細(xì)胞抑制率;RT-PCR法檢測信號(hào)轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子3(STAT3)及細(xì)胞周期蛋白D1(Cyclin D1)mRNA表達(dá)水平。結(jié)果:與NC組比較,IL-37組IL-37mRNA和蛋白表達(dá)水平明顯升高(P0.01)。給藥后24~72h,5~15mg·L-1 DDP組HeLa細(xì)胞活性受到明顯抑制(P0.01);與DDP組比較,IL-37+DDP組HeLa細(xì)胞抑制率在處理后48h內(nèi)明顯升高(P0.05);與IL-37組比較,IL-37+DDP組HeLa細(xì)胞抑制率在處理后96h內(nèi)均有升高(P0.05)。與NC組比較,IL-37組STAT3和Cyclin D1mRNA表達(dá)水平明顯下降(P0.01)。結(jié)論:IL-37高表達(dá)可抑制宮頸癌細(xì)胞的增殖,并能增強(qiáng)DDP對(duì)宮頸癌細(xì)胞的放療效果,這種效應(yīng)的發(fā)揮可能與IL-37使STAT3和Cyclin D1表達(dá)下調(diào)有關(guān)。
[Abstract]:Aim: to investigate the cytotoxicity and chemosensitivity of IL-37 to cervical cancer HeLa cells by transfection of interleukin 37 (IL-37) gene into cervical cancer HeLa cells. Methods: the recombinant pIRES2-EGFP/IL-37 plasmid (IL-37 group) and pIRES2-EGFP plasmid (NC group) were transfected into HeLa cells respectively. The expression of IL-37 gene and protein were detected by Q-PCR and Western blotting. The cell activity of NC group, IL-37 group, cisplatin (DDP) group and IL-37 DDP group were detected by CCK8, and the expression of signal transduction and transcription activator 3 (STAT3) and cyclin D1 (Cyclin D1) mRNA were detected by RT-PCR method. Results: the expression of IL-37mRNA and protein in IL-37 group was significantly higher than that in NC group (P0.01). The activity of HeLa cells in DDP group was significantly inhibited (P0.01), and the inhibition rate of HeLa cells in IL-37 DDP group was significantly higher than that in DDP group (P0.05). Compared with IL-37 group, the inhibition rate of HeLa cells in IL-37 DDP group was increased within 96 h after treatment (P0.05). Compared with NC group, the expression of STAT3 and Cyclin D1mRNA in IL-37 group decreased significantly (P0.01). Conclusion: the high expression of IL-37 can inhibit the proliferation of cervical cancer cells and enhance the radiotherapeutic effect of DDP on cervical cancer cells. This effect may be related to the down-regulation of STAT3 and Cyclin D1 expression induced by IL-37.
【作者單位】： 廣東醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院組織學(xué)與胚胎學(xué)教研室;
【基金】：國家自然科學(xué)基金資助課題(81302244) 廣東省科技廳科技發(fā)展計(jì)劃項(xiàng)目資助課題(2016A020215147,2013B021800062) 廣東省衛(wèi)計(jì)委醫(yī)學(xué)科研基金項(xiàng)目資助課題(A2016208,B2013293) 廣東省東莞市科技局社會(huì)發(fā)展重點(diǎn)基金項(xiàng)目資助課題(2013108101051) 廣東醫(yī)科大學(xué)科研基金資助課題(M2016028) 廣東省湛江市科技局科技計(jì)劃項(xiàng)目資助課題(2013B01092)
【分類號(hào)】：R737.33

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 孟迪;劉愛民;馬曉艷;盧瑋;;沉默缺氧誘導(dǎo)因子-1α對(duì)卵巢癌化療敏感性的影響[J];中國實(shí)驗(yàn)診斷學(xué);2013年06期

2 傅士龍,張國玲,李子庭,孫孟紅,李小妹;卵巢癌拓?fù)洚悩?gòu)酶Ⅱα表達(dá)與化療敏感性的關(guān)系[J];現(xiàn)代婦產(chǎn)科進(jìn)展;2002年04期

3 楊樂;張健;張t，

本文編號(hào)：2337285