【摘要】：目的觀察來曲唑灌胃后子宮內(nèi)膜異位癥(EM)大鼠病灶體積及病灶組織中環(huán)氧合酶-2(COX-2)mRNA、生存素(survivin)蛋白表達(dá)變化。方法性成熟未交配過的健康雌性Wistar大鼠48只,采用子宮內(nèi)膜自體移植法建立EM模型。選取40只造模成功的大鼠,隨機(jī)分為A、B、C、D組各10只。A、B、C組分別以來曲唑(0.26mg/kg)灌胃1、2、3周,D組以生理鹽水灌胃3周。以游標(biāo)卡尺測量給藥前后各組異位病灶體積。采用RT-PCR法檢測各組大鼠病灶組織中的COX-2 mRNA;免疫組化SP法檢測survivin蛋白。結(jié)果 A、B、C組給藥后異位病灶體積縮小,與給藥前相比,P均0.05;D組給藥前后差異無統(tǒng)計(jì)學(xué)意義。A、B、C組病灶組織中COX-2 mRNA、survivin蛋白表達(dá)與D組相比,P均0.05;A組與B、C組相比,P均0.05;B、C組差異無統(tǒng)計(jì)學(xué)意義。結(jié)論來曲唑可縮小EM大鼠異位病灶體積,并下調(diào)病灶組織中COX-2 mRNA、survivin蛋白的表達(dá),這可能是來曲唑治療EM的機(jī)制之一。
[Abstract]:Objective to observe the changes of focus volume and (survivin) protein expression of cyclooxygenase-2 (COX-2) mRNA, survivin in (EM) rats with endometriosis after oral administration of letrozole. Methods 48 healthy female Wistar rats with unmated sexual maturation were used to establish EM model by autologous transplantation of endometrium. Forty successful rats were randomly divided into two groups: group A (n = 10): 0.26mg/kg (n = 10) and group D (n = 3) were treated with normal saline for 3 weeks, respectively (n = 10), group A (n = 10) were given tritrazole (0.26mg/kg) for 3 weeks, and group D (n = 3) were given normal saline for 3 weeks. The volume of ectopic lesions in each group was measured by Vernier caliper before and after administration. The COX-2 mRNA; immunohistochemical SP method was used to detect the survivin protein in the focus tissue of rats by RT-PCR method. Results the volume of ectopic foci in group A was reduced after administration, compared with that before administration (P < 0.05). There was no significant difference in the expression of COX-2 mRNA,survivin protein between group D and group D before and after administration, and there was no significant difference in the expression of COX-2 mRNA,survivin protein between group A and group D, between group A and group B, and between group A and group B, respectively. Conclusion Letrozole can reduce the volume of ectopic focus and down-regulate the expression of COX-2 mRNA,survivin protein in EM rats, which may be one of the mechanisms of letrozole in the treatment of EM.
【作者單位】： 濰坊醫(yī)學(xué)院附屬醫(yī)院;
【分類號(hào)】：R711.71

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