發(fā)布時間：2018-10-24 08:06

【摘要】：目的探討宮頸上皮脫落細(xì)胞中mybl2 mRNA表達(dá)水平與宮頸病變程度、人乳頭瘤病毒(HPV)感染三者間的關(guān)系。方法收集宮頸上皮脫落細(xì)胞共232例,根據(jù)液基薄層細(xì)胞檢測結(jié)果對標(biāo)本進(jìn)行分組,其中不能明確意義的非典型鱗狀細(xì)胞(ASC-US)組68例,低度鱗狀上皮內(nèi)病變(LSIL)組98例,高度鱗狀上皮內(nèi)病變(HSIL)組42例,宮頸癌(SCC)組24例。Trizol法提取細(xì)胞總RNA,RT-PCR法檢測mybl2 mRNA的表達(dá)水平,分析細(xì)胞mybl2 mRNA表達(dá)水平與宮頸病變程度的關(guān)系;同時收集被檢者HPV檢測結(jié)果資料,分析細(xì)胞mybl2mRNA表達(dá)水平與HPV感染的關(guān)系。結(jié)果 (1)232例宮頸上皮脫落細(xì)胞均檢測到mybl2 mRNA表達(dá),不同病變程度的宮頸上皮脫落細(xì)胞中mybl2 mRNA表達(dá)水平差異有統(tǒng)計(jì)學(xué)意義(F=68.491,P0.01),其表達(dá)水平與宮頸病變程度呈正相關(guān),以SCC組脫落細(xì)胞的mybl2 mRNA表達(dá)水平最高。(2)宮頸脫落細(xì)胞中mybl2 mRNA表達(dá)水平與HPV感染有關(guān),差異有統(tǒng)計(jì)學(xué)意義(F=12.15,P0.01),以HPV16+和(或)HPV18+的脫落細(xì)胞表達(dá)量最高。結(jié)論宮頸上皮脫落細(xì)胞中mybl2 mRNA表達(dá)水平與宮頸病變程度呈正相關(guān),且與HPV感染有關(guān),可能與宮頸癌的疾病進(jìn)展有關(guān),有望作為宮頸病變進(jìn)展的新的生物標(biāo)志物。
[Abstract]:Objective to investigate the relationship between the expression of mybl2 mRNA and the degree of cervical lesions and the infection of human papillomavirus (HPV) in exfoliated cells of cervical epithelium. Methods 232 cases of cervical epithelial exfoliated cells were collected and divided into two groups according to the results of liquid-based thin layer cell detection. 68 cases of atypical squamous cell (ASC-US) group and 98 cases of low grade squamous intraepithelial lesion group (LSIL) group) were divided into two groups: ASC-US group (n = 68) and (LSIL) group (n = 98). There were 42 cases of high squamous intraepithelial lesions (HSIL) and 24 cases of cervical cancer (SCC). The expression level of mybl2 mRNA was detected by Trizol total RNA,RT-PCR method, and the relationship between the expression of mybl2 mRNA and the degree of cervical lesions was analyzed, and the results of HPV detection were collected. The relationship between mybl2mRNA expression and HPV infection was analyzed. Results (1) the expression of mybl2 mRNA was detected in the exfoliated cells of cervical epithelium in 232 cases. There was significant difference in the expression of mybl2 mRNA in the exfoliated cells of cervical epithelium with different degree of lesion (FF68.491p0.01), and the expression level was positively correlated with the degree of cervical lesions. The expression level of mybl2 mRNA in exfoliated cells of SCC group was the highest. (2) the expression of mybl2 mRNA in exfoliated cells of cervix uteri was related to HPV infection, and the difference was statistically significant (FF12.15, P0.01), and the expression of HPV16 and / or HPV18 in exfoliated cells was the highest. Conclusion the expression of mybl2 mRNA in the exfoliated cells of cervical epithelium is positively correlated with the degree of cervical lesions and HPV infection. It may be related to the progression of cervical cancer and may be a new biomarker for the progression of cervical lesions.
【作者單位】： 清遠(yuǎn)市人民醫(yī)院檢驗(yàn)科;清遠(yuǎn)市人民醫(yī)院婦產(chǎn)科;
【基金】：清遠(yuǎn)市科技計(jì)劃項(xiàng)目(編號:2013B042)
【分類號】：R737.33

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