發(fā)布時(shí)間：2018-10-09 07:11

【摘要】：目的·探討孕婦尿酚類(lèi)環(huán)境內(nèi)分泌干擾物檢測(cè)的尿樣收集方法,采用并比較2種方法對(duì)尿雙酚A(BPA)、三氯生(TCS)與正壬基酚(4-n-NP)檢測(cè)濃度的影響。方法·采用2種方法收集孕晚期尿樣:導(dǎo)尿管尿袋收集(n=176),聚丙烯(PP)尿杯收集(n=642)。采用高效液相色譜 串聯(lián)質(zhì)譜(HPLC-MS/MS)法檢測(cè)尿BPA、TCS及4-n-NP的濃度。驗(yàn)證實(shí)驗(yàn)采用PP材質(zhì)尿杯、導(dǎo)尿管尿袋、低密度聚乙烯材質(zhì)離心管及玻璃杯(陰性對(duì)照)留取同一婦女共5人的尿樣。結(jié)果·導(dǎo)尿管尿袋收集的尿樣BPA濃度幾何均數(shù)為82.5 ng/mL(95%CI為71.4~95.4 ng/mL),比尿杯留取尿樣的BPA濃度高63倍(幾何均數(shù)1.3 ng/mL,95%CI為1.3~1.5 ng/mL);2種方法尿TCS、4-n-NP及肌酐濃度相似。驗(yàn)證實(shí)驗(yàn)的結(jié)果表明,相同個(gè)體的尿樣,尿袋收集的尿BPA濃度遠(yuǎn)遠(yuǎn)高于其他3種方法。結(jié)論·尿酚類(lèi)環(huán)境內(nèi)分泌干擾物濃度檢測(cè)的尿樣收集,用PP材質(zhì)尿杯優(yōu)于導(dǎo)尿管尿袋收集,PP材質(zhì)尿杯及低密度聚乙烯材質(zhì)試管適合于流行病學(xué)人群研究。
[Abstract]:Objective to investigate the methods of urine sample collection for detection of environmental endocrine disruptors in urine of pregnant women, and to compare the effects of two methods on the detection of urinary bisphenol A (BPA), triclosan (TCS) and nonylphenol (4-n-NP). Methods two methods were used to collect urine samples in late pregnancy: urinary catheter bag collection (nm176), polypropylene (PP) urine cup collection (nm642). The concentrations of BPA,TCS and 4-n-NP in urine were determined by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The urine samples of 5 women were collected from PP urine cup, urinary catheter bag, low density polyethylene centrifuge tube and glass cup (negative control). Results the geometric mean of BPA concentration in urine collected by urethral catheter was 82.5 ng/mL (95%CI was 71.4 鹵95.4 ng/mL), which was 63 times higher than that in urine sample collected by urine cup (geometric mean 1.3 ng/mL,95%CI was 1.31.5 ng/mL). The results showed that the urine BPA concentration collected from the same sample was much higher than that of the other three methods. Conclusion PP urine cup is better than urethral catheter in collecting urine samples of environmental endocrine disruptor concentration of urine phenols. It is suitable for epidemiological population study to collect urethral cup made of PP and low density polyethylene (LDPE).
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬新華醫(yī)院環(huán)境與兒童健康重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(81372954,81673178) 上海市教育委員會(huì)高峰高原學(xué)科建設(shè)計(jì)劃(20152518)~~
【分類(lèi)號(hào)】：R446.12;R714
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本文編號(hào)：2258432