CIP2A和DAPK在宮頸癌中的表達(dá)及意義
發(fā)布時(shí)間：2018-10-05 17:34
【摘要】：目的：通過(guò)檢測(cè)CIP2A與DAPK在宮頸癌組織中的表達(dá)情況，分析二者在宮頸癌發(fā)生發(fā)展過(guò)程中的意義及與臨床病理因素的相關(guān)性。方法：1.收集2012年10月至2013年10月在瀘州醫(yī)學(xué)院附屬醫(yī)院婦產(chǎn)科手術(shù)切除的宮頸癌（cervical cancer，CC）病理組織標(biāo)本38例，并取同期的宮頸上皮內(nèi)瘤變（cervical intraepithelial neoplasia，CIN）組織11例及正常宮頸組織（normal cervical epithelium，NCE）10例作為對(duì)照組。所有病人在取宮頸組織標(biāo)本前均未接受過(guò)化療或放療，臨床及病理資料完整。標(biāo)本均經(jīng)10%福爾馬林固定，常規(guī)石蠟包埋，連續(xù)切片，片厚4μm，做HE染色和免疫組化染色。病理診斷均由有經(jīng)驗(yàn)的病理科專(zhuān)家復(fù)核。三組病例的平均年齡分別為：正常宮頸組44.80±12.57歲，CIN組48.82±11.05歲，宮頸癌組45.47±7.31歲。2.用免疫組化Envision方法檢測(cè)CIP2A、DAPK在正常宮頸、宮頸上皮內(nèi)瘤變及宮頸癌組織中的表達(dá)情況。3.實(shí)驗(yàn)結(jié)果采用ImageproPlus5.0軟件分析圖片平均光密度（mean optical density，MOD），得出數(shù)據(jù)采用獨(dú)立樣本t檢驗(yàn)，相關(guān)性分析采用Spearman等級(jí)相關(guān)分析，取用雙側(cè)檢驗(yàn)，P＜0.05為有統(tǒng)計(jì)學(xué)意義。結(jié)果：1.CIP2A蛋白在不同宮頸組織中的陽(yáng)性反應(yīng)主要分布在細(xì)胞膜和（或）細(xì)胞質(zhì)，CIP2A在正常宮頸、CIN、宮頸癌組織中測(cè)定的MOD值分別為10.27±18.19、46.71±39.23、61.96±39.06，總體隨病變嚴(yán)重程度加重呈升高趨勢(shì)，將之MOD值分別兩兩進(jìn)行t檢驗(yàn)，，結(jié)果：在正常宮頸與CIN中CIP2A的表達(dá)差異有統(tǒng)計(jì)學(xué)意義（P=0.015＜0.05），在正常宮頸與宮頸癌中CIP2A的表達(dá)差異有統(tǒng)計(jì)學(xué)意義（P＜0.001）;在CIN與宮頸癌中CIP2A的表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）；在宮頸癌患者中，CIP2A的表達(dá)在不同浸潤(rùn)深度組中差異有統(tǒng)計(jì)學(xué)意義（P＜0.05），在不同腫瘤大小、不同組織類(lèi)型、不同分化程度、不同臨床分期、淋巴結(jié)轉(zhuǎn)移陽(yáng)性和陰性組、脈管內(nèi)是否見(jiàn)癌栓組以及不同年齡組中表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）。2.DAPK蛋白在正常宮頸組織中的陽(yáng)性反應(yīng)主要分布在細(xì)胞核和（或）細(xì)胞質(zhì)，在CIN及宮頸癌組織中陽(yáng)性反應(yīng)主要在細(xì)胞膜和（或）細(xì)胞質(zhì)；陽(yáng)性反應(yīng)均表現(xiàn)為不同程度的黃色或棕黃色著色。DAPK在正常宮頸、CIN、宮頸癌組中測(cè)定的MOD值進(jìn)行K-S正態(tài)檢驗(yàn)發(fā)現(xiàn)其不符合正態(tài)分布，將其進(jìn)行開(kāi)方轉(zhuǎn)換后再次檢驗(yàn)符合正態(tài)分布，轉(zhuǎn)換后DAPK在正常宮頸、CIN、宮頸癌組中的MOD值依次為9.04±3.47、9.04±3.47、3.74±3.30，總體隨病變嚴(yán)重程度加重呈降低趨勢(shì)，將之分別兩兩進(jìn)行t檢驗(yàn)，結(jié)果：在正常宮頸與CIN中DAPK的表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）；在正常宮頸與宮頸癌中DAPK的表達(dá)差異有統(tǒng)計(jì)學(xué)意義（P＜0.001）;在CIN與宮頸癌中DAPK的表達(dá)差異有統(tǒng)計(jì)學(xué)意義（P＜0.001）。在宮頸癌患者中，DAPK的表達(dá)在腫瘤最大徑線(xiàn)≥4cm和＜4cm組表達(dá)差異有統(tǒng)計(jì)學(xué)意義（P=0.015＜0.05）。以35歲為界進(jìn)行t檢驗(yàn)，發(fā)現(xiàn)DAPK在≥35歲組與＜35歲組間表達(dá)差異有統(tǒng)計(jì)學(xué)意義（P=0.026＜0.05），在不同組織類(lèi)型、不同分化程度、不同浸潤(rùn)深度、不同臨床分期、淋巴結(jié)轉(zhuǎn)移陽(yáng)性和陰性組、脈管內(nèi)是否見(jiàn)癌栓組中表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）。3.二者的相關(guān)性：CIP2A與DAPK在宮頸組織中的表達(dá)強(qiáng)度無(wú)相關(guān)性（P＞0.05）。結(jié)論：1.CIP2A在宮頸癌中過(guò)表達(dá)，DAPK在宮頸癌中表達(dá)缺失（或減少）。2.CIP2A的表達(dá)增高和DAPK的表達(dá)降低可能與宮頸癌的發(fā)生有關(guān)。3.CIP2A和DAPK分別通過(guò)不同途徑影響宮頸癌的發(fā)生發(fā)展，相互表達(dá)無(wú)相關(guān)性。
[Abstract]:Objective: To analyze the expression of CIP2A and DAPK in cervical cancer tissues, and to analyze the significance of both in the development of cervical cancer and the correlation with clinical pathological factors. Method: 1. 38 cases of cervical carcinoma (CC) were collected from October 2012 to October 2013 in the department of obstetrics and gynecology in Luzhou Medical College, and 11 cases of cervical intraepithelial neoplasia (CIN) and normal cervical tissue were taken. NCE) 10 cases were treated as control group. All patients had not received chemotherapy or radiotherapy before taking cervical tissue specimens, and the clinical and pathological data were complete. The specimens were fixed with 10% formalin, conventional paraffin embedding, continuous slicing, thickness of 4. m u.m, HE staining and immunohistochemical staining. Pathological diagnosis was reviewed by experienced pathologist. The mean age of the three cases were: normal cervical group (44. 80) 12. 57 years old, CIN group (48. 82) 11. 05 years old, cervical cancer group (45. 47) 7. 31 years old. The expression of CIP2A and DAPK in normal cervical, cervical intraepithelial neoplasia and cervical carcinoma were detected by immunohistochemistry. The average optical density (MOD) of image was analyzed by ImageptroPlus5.0 software. The data was tested by independent sample t. The correlation analysis was analyzed by Spearman grade, and the two-side test was taken, and P <0.05 was statistical significance. Results: 1. The positive reaction of CIP2A protein in different cervical tissues was mainly distributed in cell membrane and (or) cytoplasm. The MOD values measured in the tissues of normal cervix, CIN and cervical carcinoma were 10.27, 18. 19, 46. 71, 39. 23, 61. 96 and 39. 06, respectively. The results showed that there was a significant difference in the expression of CIP2A between normal cervix and CIN (P = 0.0015 <0.05), and the expression of CIP2A in normal cervical and cervical carcinoma was statistically significant (P <0.001). There was no significant difference in the expression of CIP2A between CIN and cervical carcinoma (P> 0.05). In patients with cervical cancer, the expression of CIP2A was statistically significant (P <0.05). There was no significant difference in the expression of DAPK in normal cervical tissues (P> 0.05). The positive reaction of DAPK protein in normal cervical tissues was mainly distributed in nucleus and/ or cytoplasm. In CIN and cervical carcinoma tissues, the positive reaction was mainly in the cell membrane and (or) cytoplasm; the positive reaction showed a different degree of yellow or brown yellow coloration. The MOD value of DIAPK in normal cervix, CIN and cervical cancer group was determined by K-S positive state test. It was found that it was not in accordance with normal distribution, and the normal distribution was again tested after it was switched on. The MOD value of DIAPK in normal cervix, CIN and cervical cancer group was 9.04, 3.47, 9.04, 3.47, 3.74, 3.30, respectively. Results: There was no significant difference in the expression of DAPK in normal cervix and CIN (P> 0.05), and the difference of DAPK expression in normal cervical and cervical carcinoma was statistically significant (P <0.001). There was significant difference in the expression of DAPK in CIN and cervical carcinoma (P <0.001). In patients with cervical cancer, the expression of DAPK was statistically significant (P = 0. 015 <0.05). At the age of 35, the expression of DAPK in the 35-year-old group and <35-year-old group was statistically significant (P = 0. 0. 05). In different tissue types, different differentiation degrees, different infiltration depths, different clinical stages, lymph node metastasis and negative group, There was no significant difference in the expression of tumor thrombus in the vessel (P> 0.05). There was no correlation between the expression intensity of CIP2A and DIAPK in cervical tissues (P> 0.05). Conclusion: 1. CIP2A has been expressed in cervical carcinoma. The expression of DIAPK in cervical carcinoma is absent (or decreased). The expression of CIP2A and the decrease of DAPK may be related to the occurrence of cervical cancer.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R737.33
本文編號(hào)：2254251
[Abstract]:Objective: To analyze the expression of CIP2A and DAPK in cervical cancer tissues, and to analyze the significance of both in the development of cervical cancer and the correlation with clinical pathological factors. Method: 1. 38 cases of cervical carcinoma (CC) were collected from October 2012 to October 2013 in the department of obstetrics and gynecology in Luzhou Medical College, and 11 cases of cervical intraepithelial neoplasia (CIN) and normal cervical tissue were taken. NCE) 10 cases were treated as control group. All patients had not received chemotherapy or radiotherapy before taking cervical tissue specimens, and the clinical and pathological data were complete. The specimens were fixed with 10% formalin, conventional paraffin embedding, continuous slicing, thickness of 4. m u.m, HE staining and immunohistochemical staining. Pathological diagnosis was reviewed by experienced pathologist. The mean age of the three cases were: normal cervical group (44. 80) 12. 57 years old, CIN group (48. 82) 11. 05 years old, cervical cancer group (45. 47) 7. 31 years old. The expression of CIP2A and DAPK in normal cervical, cervical intraepithelial neoplasia and cervical carcinoma were detected by immunohistochemistry. The average optical density (MOD) of image was analyzed by ImageptroPlus5.0 software. The data was tested by independent sample t. The correlation analysis was analyzed by Spearman grade, and the two-side test was taken, and P <0.05 was statistical significance. Results: 1. The positive reaction of CIP2A protein in different cervical tissues was mainly distributed in cell membrane and (or) cytoplasm. The MOD values measured in the tissues of normal cervix, CIN and cervical carcinoma were 10.27, 18. 19, 46. 71, 39. 23, 61. 96 and 39. 06, respectively. The results showed that there was a significant difference in the expression of CIP2A between normal cervix and CIN (P = 0.0015 <0.05), and the expression of CIP2A in normal cervical and cervical carcinoma was statistically significant (P <0.001). There was no significant difference in the expression of CIP2A between CIN and cervical carcinoma (P> 0.05). In patients with cervical cancer, the expression of CIP2A was statistically significant (P <0.05). There was no significant difference in the expression of DAPK in normal cervical tissues (P> 0.05). The positive reaction of DAPK protein in normal cervical tissues was mainly distributed in nucleus and/ or cytoplasm. In CIN and cervical carcinoma tissues, the positive reaction was mainly in the cell membrane and (or) cytoplasm; the positive reaction showed a different degree of yellow or brown yellow coloration. The MOD value of DIAPK in normal cervix, CIN and cervical cancer group was determined by K-S positive state test. It was found that it was not in accordance with normal distribution, and the normal distribution was again tested after it was switched on. The MOD value of DIAPK in normal cervix, CIN and cervical cancer group was 9.04, 3.47, 9.04, 3.47, 3.74, 3.30, respectively. Results: There was no significant difference in the expression of DAPK in normal cervix and CIN (P> 0.05), and the difference of DAPK expression in normal cervical and cervical carcinoma was statistically significant (P <0.001). There was significant difference in the expression of DAPK in CIN and cervical carcinoma (P <0.001). In patients with cervical cancer, the expression of DAPK was statistically significant (P = 0. 015 <0.05). At the age of 35, the expression of DAPK in the 35-year-old group and <35-year-old group was statistically significant (P = 0. 0. 05). In different tissue types, different differentiation degrees, different infiltration depths, different clinical stages, lymph node metastasis and negative group, There was no significant difference in the expression of tumor thrombus in the vessel (P> 0.05). There was no correlation between the expression intensity of CIP2A and DIAPK in cervical tissues (P> 0.05). Conclusion: 1. CIP2A has been expressed in cervical carcinoma. The expression of DIAPK in cervical carcinoma is absent (or decreased). The expression of CIP2A and the decrease of DAPK may be related to the occurrence of cervical cancer.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R737.33
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 韓麗華;蘇秋香;;非小細(xì)胞肺癌中CIP2A的表達(dá)及與臨床病理的相關(guān)性[J];臨床與實(shí)驗(yàn)病理學(xué)雜志;2013年07期
本文編號(hào)：2254251
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