【摘要】：目的：分析宮腔子宮內(nèi)膜樣腺癌和卵巢子宮內(nèi)膜樣腺癌兩部位癌組織中端粒長(zhǎng)度是否相近,ER、PR及P53的表達(dá)是否相似,探討兩部位癌組織是否有同源性。 方法：收集子宮內(nèi)膜和卵巢原發(fā)雙癌患者的癌組織切除標(biāo)本16例,子宮內(nèi)膜癌組織切除標(biāo)本43例,卵巢癌組織切除標(biāo)本10例,采用熒光定量PCR法分別檢測(cè)16例原發(fā)雙癌患者兩部位癌組織中端粒DNA的相對(duì)表達(dá)量,并采用免疫組化PV-6000二步法檢測(cè)所有癌組織切除標(biāo)本中免疫表型雌激素受體(Estrogen receptor ER)、孕激素受體(Progesterone receptor PR)及p53的表達(dá)。結(jié)果：(1)16例原發(fā)雙癌患者,子宮內(nèi)膜部位癌組織端粒相對(duì)長(zhǎng)度(3.601±1.497)與卵巢部位癌組織端粒相對(duì)長(zhǎng)度(3.71±1.556)相近,差異無(wú)統(tǒng)計(jì)學(xué)意義(t=1.423,P=0.175)。(2)原發(fā)雙癌兩部位癌組織ER蛋白免疫表型相同10例(62.5%),免疫表型不同6例(37.5%),差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.06);PR蛋白免疫表型相同9例(56.25%),免疫表型不同7例(43.75%),差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.09)；P53蛋白免疫表型相同9例(56.25%),免疫表型不同7例(43.75%),差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.36)。(3)ER蛋白在單發(fā)性子宮內(nèi)膜癌與原發(fā)雙癌子宮內(nèi)膜部位癌組織中(P=0.65)及單發(fā)性卵巢癌與原發(fā)雙癌卵巢部位癌組織中(P=0.53)的表達(dá)差異均無(wú)統(tǒng)計(jì)學(xué)意義；PR蛋白在單發(fā)性子宮內(nèi)膜癌與原發(fā)雙癌子宮內(nèi)膜部位癌組織中(P=0.48)及單發(fā)性卵巢癌與原發(fā)雙癌卵巢部位癌組織中(P=0.42)的表達(dá)差異均無(wú)統(tǒng)計(jì)學(xué)意義；P53蛋白在單發(fā)性子宮內(nèi)膜癌與原發(fā)雙癌子宮內(nèi)膜部位癌組織中(P=0.03)及單發(fā)性卵巢癌與原發(fā)雙癌卵巢部位癌組織中(P=0.04)的表達(dá)差異均有統(tǒng)計(jì)學(xué)意義。 結(jié)論：(1)宮腔子宮內(nèi)膜和卵巢兩部位癌組織端粒長(zhǎng)度相近,ER, PR及P53在兩部位癌組織中的表達(dá)也相似。由此推斷兩部位癌組織可能有相同的組織來源,尚需進(jìn)一步研究證實(shí)。 (2)ER、PR在單發(fā)性子宮內(nèi)膜癌、卵巢癌中的表達(dá)與原發(fā)雙癌相應(yīng)部位癌組織中的表達(dá)相似,P53在單發(fā)性子宮內(nèi)膜癌、卵巢癌中的表達(dá)與原發(fā)雙癌相應(yīng)部位癌組織中的表達(dá)均不同。因此,原發(fā)雙癌與相應(yīng)部位單發(fā)癌組織發(fā)生癌變的分子機(jī)制可能存在一定差異。
[Abstract]:Objective: to investigate whether the expression of telomere length in endometrial adenocarcinoma and ovarian endometrioid adenocarcinoma is similar to that of ERP PR and p53, and to explore whether there is homology between the two tissues. Methods: 16 specimens of carcinoma, 43 specimens of endometrial carcinoma and 10 specimens of ovarian cancer were collected from patients with primary carcinoma of endometrium and ovary. The relative expression of telomere DNA was detected by fluorescence quantitative PCR in 16 patients with primary double carcinomas. The expression of estrogen receptor (Estrogen receptor ER), progesterone receptor (Progesterone receptor PR) and p53 were detected by immunohistochemical PV-6000 two-step method. Results: (1) the relative length of telomere in endometrial carcinoma (3.601 鹵1.497) and ovarian carcinoma (3.71 鹵1.556) was similar to that in 16 patients with primary double cancer. The immunophenotype of ER protein was the same in 10 cases (62.5%) and different in 6 cases (37.5%). There was no significant difference in the immunophenotype of PR protein in 9 cases (56.25%), and in 7 cases (43.75%). There was no statistical significance (P0. 09). The immunophenotype of p53 protein was the same in 9 cases (56.25%), and the immunophenotype was different in 7 cases (43.75%). There was no significant difference (P0. 36). (3) in the expression of p53 protein in endometrial tissues of single endometrial carcinoma and primary double carcinomas (P0. 65), and between single ovarian carcinoma and primary double carcinoma. There was no significant difference in the expression of P0. 53 in the tissues of nests. There was no significant difference in the expression of PR protein between primary and single endometrial carcinomas (P0. 48) and primary ovarian cancer (P0. 42). There were significant differences in the expression of p53 protein between single endometrial carcinoma and primary double carcinoma of endometrium (P0. 03) and between single ovarian cancer and primary bicarcinoma of ovary (P0. 04). Conclusion: (1) the telomere length of endometrial and ovarian carcinoma is similar, and the expression of PR and p53 is similar. It is concluded that there may be the same tissue origin in the two sites of carcinoma, and further research is needed. (2) ER,PR in single endometrial carcinoma, The expression of p53 was similar to that in the corresponding tissues of primary double carcinomas, while the expression of p53 in ovarian cancer was different from that in the corresponding tissues of primary double carcinomas. Therefore, there may be some differences in the molecular mechanism of carcinogenesis between primary double carcinomas and corresponding single carcinomas.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R737.33;R737.31

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