BKCa、SK3、TREK-1鉀離子通道在晚孕大鼠子宮中的表達及功能研究
發(fā)布時間：2018-08-27 10:55
【摘要】：目的:探討大電導鈣激活鉀通道(BKCa)、小電導鈣激活鉀通道(SK3)以及雙孔鉀離子通道(TREK-1)在非孕和晚孕大鼠子宮中的表達,以及對子宮舒縮功能的影響。方法:將SD大鼠分為非孕組(NP)和晚孕組(孕19天,LP),采用Western blot法和q PCR法檢測非孕和晚孕大鼠子宮組織中3種收縮相關(guān)鉀離子通道(BKCa、SK3、TREK-1)蛋白和基因表達水平。利用等長收縮實驗,觀察比較兩組子宮肌條在縮宮素誘導下的收縮能力變化以及TREK-1通道抑制劑對晚孕組子宮肌條收縮能力的影響。結(jié)果:與非孕組比較,晚孕組子宮組織中3種收縮相關(guān)鉀離子通道(BKCa、SK3、TREK-1)蛋白表達水平和基因表達水平顯著提高(P0.05),晚孕組肌條在縮宮素誘導下的收縮力顯著降低(P0.05)。TREK-1通道抑制劑L-甲硫氨酸可顯著提高晚孕組子宮肌條收縮能力(P0.05)。結(jié)論:晚孕子宮靜息狀態(tài)的維持與BKCa、SK3以及TREK-1通道表達密切相關(guān),TREK-1通道可能通過調(diào)節(jié)子宮收縮能力參與分娩發(fā)動。
[Abstract]:Aim: to investigate the expression of large conductance calcium activated potassium channel (BKCa), small conductance calcium activated potassium channel (SK3) and double pore potassium ion channel (TREK-1) in the uterus of non-pregnant and late pregnant rats, and their effects on the uterine function. Methods: SD rats were divided into non-pregnant group (NP) and late pregnancy group (19-day LP). Western blot and Q PCR methods were used to detect three kinds of contractile potassium channel (BKCa,SK3,TREK-1) protein and gene expression in the uterus of non-pregnant and late pregnant rats. The contractile ability of uterine muscle strips induced by oxytocin and the effect of TREK-1 channel inhibitor on the contractility of uterine muscle strips in late pregnancy group were observed and compared by isometric contraction experiment. Results: compared with the non-pregnant group, The expression of three contractile associated potassium channels (BKCa,SK3,TREK-1) protein and gene expression were significantly increased in the uterine tissues of the late pregnancy group (P0.05), and the contractile force of the muscle strips in the late pregnancy group was significantly decreased under oxytocin induction (P0.05). L- methionine, a channel inhibitor of TREK-1, was significantly decreased in the late pregnancy group (P0.05). The contractility of uterine muscle strips in late pregnancy group was significantly increased (P0.05). Conclusion: the maintenance of uterine resting state in late pregnancy is closely related to the expression of BKCa,SK3 and TREK-1 channels, and the TREK-1 channel may be involved in labor initiation by regulating uterine contractility.
【作者單位】： 安徽醫(yī)科大學第一附屬醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學基金青年科學基金(No:81300514) 中國博士后科學基金(No:2016M592039) 安徽省博士后研究人員科研活動基金(No:2015B080)
【分類號】：R714
，
本文編號：2207060
[Abstract]:Aim: to investigate the expression of large conductance calcium activated potassium channel (BKCa), small conductance calcium activated potassium channel (SK3) and double pore potassium ion channel (TREK-1) in the uterus of non-pregnant and late pregnant rats, and their effects on the uterine function. Methods: SD rats were divided into non-pregnant group (NP) and late pregnancy group (19-day LP). Western blot and Q PCR methods were used to detect three kinds of contractile potassium channel (BKCa,SK3,TREK-1) protein and gene expression in the uterus of non-pregnant and late pregnant rats. The contractile ability of uterine muscle strips induced by oxytocin and the effect of TREK-1 channel inhibitor on the contractility of uterine muscle strips in late pregnancy group were observed and compared by isometric contraction experiment. Results: compared with the non-pregnant group, The expression of three contractile associated potassium channels (BKCa,SK3,TREK-1) protein and gene expression were significantly increased in the uterine tissues of the late pregnancy group (P0.05), and the contractile force of the muscle strips in the late pregnancy group was significantly decreased under oxytocin induction (P0.05). L- methionine, a channel inhibitor of TREK-1, was significantly decreased in the late pregnancy group (P0.05). The contractility of uterine muscle strips in late pregnancy group was significantly increased (P0.05). Conclusion: the maintenance of uterine resting state in late pregnancy is closely related to the expression of BKCa,SK3 and TREK-1 channels, and the TREK-1 channel may be involved in labor initiation by regulating uterine contractility.
【作者單位】： 安徽醫(yī)科大學第一附屬醫(yī)院婦產(chǎn)科;
【基金】：國家自然科學基金青年科學基金(No:81300514) 中國博士后科學基金(No:2016M592039) 安徽省博士后研究人員科研活動基金(No:2015B080)
【分類號】：R714
，
本文編號：2207060
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