【摘要】：研究背景及目的：妊娠期高血壓疾病是妊娠期特發(fā)的疾病，是最常見孕期并發(fā)癥，也孕產(chǎn)婦和圍生兒死亡率升高的主要原因之一。但是其發(fā)病機(jī)制尚未完全闡明。細(xì)胞黏附分子(cell adhesionmolecule, CAM)在免疫應(yīng)答、對移植物的排斥反應(yīng)以及在血管內(nèi)皮活化的過程中起著重要作用。很多研究表明，CAM與妊娠期高血壓疾病的發(fā)病有著密切聯(lián)系。本研究旨在研究母胎界面中2種重要的CAM分子：細(xì)胞間粘附因子-1（cell adhesion molecule-1, ICAM-1）和血管細(xì)胞粘附因子-1（vascular cell adhesion molecule-1, VCAM-1）在妊娠期高血壓疾病發(fā)病中的作用，為妊娠期高血壓疾病的預(yù)防和治療提供科學(xué)依據(jù)。 材料和方法：收集10例正常的產(chǎn)婦、20例輕度子癇前期產(chǎn)婦、20例重度子癇前期產(chǎn)婦的胎盤及底蛻膜組織：采用免疫組織化學(xué)方法分別測定母胎界面（胎盤和底蛻膜）中ICAM-1和VCAM-1的表達(dá)，免疫組化完成之后，采用Olympus DP21顯微鏡進(jìn)行圖片采集，每張切片在400倍高倍鏡下隨機(jī)選擇切片的5個視野。并采用圖像分析系統(tǒng)(Image-pro plus5.0軟件)測定圖片累積光密度值（integraloptical density，IOD），取其平均值。然后采用SPSS18.0統(tǒng)計軟件，，對各組的數(shù)據(jù)進(jìn)行比較。采用方差分析的方法比較各組間兩種因子表達(dá)的強(qiáng)度的差異.各組臨床資料與ICAM-1和VCAM-1表達(dá)強(qiáng)度的相關(guān)性。 結(jié)論：1. ICAM-1和VCAM-1在正常妊娠、輕度子癇前期、重度子癇前期底蛻膜組織中均有表達(dá)；2. ICAM-1和VCAM-1在妊娠期高血壓疾病胎盤組織中表達(dá)高于正常胎盤組織；其中重度子癇前期組胎盤組織中的ICAM-1和VCAM-1較輕度子癇前期組升高更明顯。
[Abstract]:Background and objective: hypertensive disorder complicating pregnancy is one of the most common complications during pregnancy and one of the main reasons for the increase of maternal and perinatal mortality. However, its pathogenesis has not been fully elucidated. Cell adhesion molecule (cell adhesionmolecule, CAM) plays an important role in immune response, graft rejection and vascular endothelial activation. Many studies have shown that CAM is closely related to the pathogenesis of hypertensive disorders complicating pregnancy. The purpose of this study was to investigate the role of two important CAM molecules in maternal and fetal interface: intercellular adhesion factor-1 (cell adhesion molecule-1, ICAM-1 (ICAM-1) and vascular cell adhesion factor-1 (vascular cell adhesion molecule-1, VCAM-1 (VCAF-1) in the pathogenesis of hypertensive disorder complicating pregnancy. To provide scientific basis for the prevention and treatment of hypertensive disorder complicating pregnancy. Materials and methods: placenta and decidua of 20 women with severe preeclampsia were collected from 10 normal women with mild preeclampsia. The maternal and fetal interfaces (placenta and decidua) were measured by immunohistochemical method. For the expression of ICAM-1 and VCAM-1, After the completion of immunohistochemistry, the images were collected by Olympus DP21 microscope. Each slice was randomly selected for 5 visual fields under a 400-fold high-power microscope. The cumulative optical density (integraloptical density,IOD) of images was measured by image analysis system (Image-pro plus5.0), and the average value was calculated. Then SPSS18.0 statistical software was used to compare the data of each group. ANOVA was used to compare the intensity of expression of two kinds of factors in each group. The correlation between the clinical data and the expression of ICAM-1 and VCAM-1 in each group. Conclusion 1. ICAM-1 and VCAM-1 were expressed in decidua of normal pregnancy, mild preeclampsia and severe preeclampsia. The expression of ICAM-1 and VCAM-1 in placenta of hypertensive disorder complicating pregnancy was higher than that of normal placenta, and the ICAM-1 and VCAM-1 in placenta of severe preeclampsia were higher than those of mild preeclampsia.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.246

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相關(guān)期刊論文 前2條

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2 蔡煙岫,耿淑平,劉曼華,謝曉謙;內(nèi)皮細(xì)胞粘附分子在妊高征發(fā)病中的作用[J];南通醫(yī)學(xué)院學(xué)報;2004年03期

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