【摘要】：目的:探討細胞命運決定因子DACH1(dachshund family transcription factor 1)對人子宮內膜癌HEC-1A細胞體外增殖能力和細胞周期的影響。方法 :將DACH1過表達的重組慢病毒LV-DACH1-GFP感染人子宮內膜癌HEC-1A細胞,在激光共聚焦顯微鏡下觀察DACH1在HEC-1A細胞中的定位;分別采用實時熒光定量PCR法和蛋白質印跡法檢測HEC-1A細胞中DACH1 m RNA和蛋白的表達,MTT法檢測HEC-1A細胞的體外的增殖能力,FCM法檢測細胞周期的分布。結果 :重組慢病毒LV-DACH1-GFP感染人子宮內膜癌HEC-1A細胞后,LV-DACH1-GFP主要分布在細胞核中。LV-DACH1-GFP感染組HEC-1A細胞中,DACH1 m RNA和蛋白的表達水平均明顯高于對照組(HEC-1A細胞感染慢病毒LV-GFP)和空白組(HEC-1A細胞未進行任何感染)(P值均0.01),LV-DACH1-GFP感染組HEC-1A細胞的增殖受到明顯抑制(P0.05),細胞周期阻滯于G0/G1期(P0.01)。結論 :D ACH1基因可抑制人子宮內膜癌HEC-1A細胞的增殖,這一作用可能與DACH1基因阻滯細胞周期有關。
[Abstract]:Aim: to investigate the effect of DACH1 (dachshund family transcription factor 1 on the proliferation and cell cycle of human endometrial carcinoma HEC-1A cells in vitro. Methods: human endometrial carcinoma HEC-1A cells were infected with recombinant lentivirus LV-DACH1-GFP which was overexpressed by DACH1. The localization of DACH1 in HEC-1A cells was observed under confocal laser microscope. The expression of DACH1 m RNA and protein in HEC-1A cells were detected by real-time fluorescence quantitative PCR assay and Western blotting respectively. The proliferation of HEC-1A cells in vitro was detected by FCM method and the cell cycle distribution was detected by FCM method. Results: after recombinant lentivirus LV-DACH1-GFP was infected with human endometrial carcinoma HEC-1A cells, the expression levels of RNA and protein in HEC-1A cells of LV-DACH1-GFP infection group were significantly higher than those of control group (HEC-1A cells infected with lentivirus LV-GFP) and empty cells (LV-DACH1-GFP). The proliferation of LV-DACH1-GFP infected HEC-1A cells was significantly inhibited (P0.05), and the cell cycle was blocked in G0/G1 phase (P0.01). ConclusionThe cell proliferation of human endometrial carcinoma HEC-1A cells can be inhibited by DACH1 D ACH1 gene, which may be related to the arrest of cell cycle by DACH1 gene.
【作者單位】： 山東中醫(yī)藥大學藥學院天然藥物重點實驗室;
【基金】：國家自然科學基金資助項目(編號:81173614)~~
【分類號】：R737.33

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