【摘要】：目的探討精子-透明質(zhì)酸結合率對受精能力的預測以及妊娠的影響。方法回顧性分析2016年1月—6月期間本中心正常受精的短時體外受精(IVF)患者1 630例作為對照組(短時IVF組),因短時受精失敗行補救卵胞質(zhì)內(nèi)單精子注射(ICSI)的130例作為研究組(R-ICSI組),檢測并分析兩組精液的精子-透明質(zhì)酸結合率、濃度、前向運動率、正常形態(tài)率、胚胎質(zhì)量以及妊娠結局。結果 (1)女方年齡、不孕年限、總Gn用量、Gn使用時間以及獲卵數(shù)組間均無統(tǒng)計學差異(P0.05);(2)R-ICSI組的精子-透明質(zhì)酸結合率[(63.8±17.4)%]顯著低于短時IVF組[(71.8±13.6)%](P0.01),并同時伴有精子濃度和正常形態(tài)率的明顯下降(P0.05),但其均值卻仍然高于精液常規(guī)參數(shù)的正常參考值;(3)R-ICSI組的正常受精率(70.8%)、總受精率(76.2%)及優(yōu)質(zhì)胚胎率(50.0%)均顯著低于短時IVF組(76.01%,85.0%,59.0%)(P0.01);(4)R-ICSI組平均移植胚胎數(shù)(2.0±0.2)顯著高于短時IVF組(1.9±0.3)(P0.01),但種植率(36.6%)卻仍然低于短時IVF組(49.0%)(P0.05);R-ICSI組臨床妊娠率低于短時IVF組,胚胎停育率高于短時IVF組,但兩者之間的差異并無統(tǒng)計學意義(P0.05)。結論精子-透明質(zhì)酸結合率對IVF有一定的預測作用,結合常規(guī)參數(shù),精子-透明質(zhì)酸結合率低于63.8%時,受精失敗風險增加,但其對于妊娠的影響還有待于進一步研究。
[Abstract]:Objective to study the effect of sperm-hyaluronic acid binding rate on fertilization ability and pregnancy. Methods from January to June 2016, 1 630 (IVF) patients with normal fertilization in our center were used as control group (short term IVF group). 130 cases of (ICSI) were injected into the oocyte due to the failure of short term fertilization. As a study group (R-ICSI group), sperm-hyaluronic acid binding rates were measured and analyzed in both groups. Concentration, forward motion rate, normal form rate, embryo quality, and pregnancy outcome. Results (1) the age of the woman, the length of her infertility, The sperm hyaluronic acid binding rate of R-ICSI group [(63.8 鹵17.4)%] was significantly lower than that of short-term IVF group [(71.8 鹵13.6)%] (P0.01), accompanied by a significant decrease in sperm concentration and normal morphology (P0.05). (3) the normal fertilization rate (70.8%), total fertilization rate (76.2%) and high quality embryo rate (50.0%) in R-ICSI group were significantly lower than those in short-term IVF group (76.01%) (85.0%) (P0.01); (4) and the average number of embryos transferred in R-ICSI group (2.0 鹵0.2) was significantly higher than that in short-term IVF group (1.9 鹵0.3) (P0.01). The clinical pregnancy rate of R-ICSI group (36.6%) was still lower than that of short-term IVF group (49.0%) (P0.05), and the clinical pregnancy rate of R-ICSI group was lower than that of short-term IVF group. The embryo suspension rate was higher than that in the short term IVF group, but there was no significant difference between the two groups (P0.05). Conclusion the sperm hyaluronic acid binding rate can predict IVF to some extent. When the sperm hyaluronic acid binding rate is lower than 63.8, the risk of fertilization failure increases, but the effect on pregnancy needs further study.
【作者單位】： 深圳市武警醫(yī)院生殖中心;
【分類號】：R714.8

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