胎膜早破患者基質(zhì)金屬蛋白酶-9及其底物水平表達(dá)
發(fā)布時間：2018-07-20 11:00
【摘要】：目的檢測足月胎膜早破孕婦血漿鋅、血清及羊水MMP-9、血清及羊水IV型膠原含量變化及胎膜IV型膠原表達(dá)水平，分析鋅、MMP-9和IV型膠原之間的相關(guān)性，探討基質(zhì)金屬蛋白酶-9及其底物水平與足月胎膜早破的關(guān)系，為胎膜早破的發(fā)病病因及預(yù)防提供理論依據(jù)。 方法選擇自2012年11月-2013年3月我院確診為足月胎膜早破患者30例作為病例組，另選擇在同一時期足月分娩的非胎膜早破患者30例作為對照組，收集所有研究對象的孕母外周血、羊水及胎膜組織，分別應(yīng)用原子吸收法檢測血漿鋅含量，應(yīng)用ELISA法檢測血清和羊水MMP-9含量、應(yīng)用上轉(zhuǎn)發(fā)光法檢測血清和羊水IV型膠原含量，用免疫組化法檢測病例組與對照組胎膜IV型膠原表達(dá)，應(yīng)用奧林巴斯Bx63全自動顯微鏡掃描系統(tǒng)觀察和掃描，比較病例組與對照組上述指標(biāo)表達(dá)水平的差異，并對各指標(biāo)之間的相關(guān)性進(jìn)行分析。 結(jié)果1、胎膜早破組孕婦血漿鋅含量平均為109.10umol/l,顯著高于對照組，差異有統(tǒng)計學(xué)意義（P＜0.05）；胎膜早破組孕婦血清MMP-9含量平均為1463.25ng/ml，顯著高于對照組，差異有統(tǒng)計學(xué)意義（P＜0.05），羊水MMP-9含量平均為125.48ng/ml，顯著高于對照組，差異有統(tǒng)計學(xué)意義（P＜0.05）；胎膜早破組孕婦血清IV型膠原含量平均為56.86ng/ml，顯著低于對照組，差異有統(tǒng)計學(xué)意義（P＜0.05）；胎膜早破組孕婦羊水IV型膠原含量明顯高于正常對照組，P＜0.05，差異有統(tǒng)計學(xué)意義（P＜0.05）；2、孕婦血清MMP-9、羊水MMP-9之間有相關(guān)性，呈正相關(guān)（P＜0.05）；孕婦血漿鋅、血清MMP-9之間有相關(guān)性，呈正相關(guān)（P＜0.05）；孕婦血漿鋅與血清IV型膠原之間有相關(guān)性，呈負(fù)相關(guān)（P＜0.05）；孕婦血漿鋅與羊水MMP-9、血清IV型膠原與血清MMP-9、血清IV型膠原與羊水MMP-9之間均無相關(guān)性（P＞0.05），孕婦血漿鋅、血清IV型膠原與胎膜IV型膠原之間均無相關(guān)性（P＞0.05）；孕婦血清MMP-9、羊水MMP-9與胎膜IV型膠原之間有相關(guān)性，呈負(fù)相關(guān)（P＜0.05）；3、絨毛膜羊膜炎時，胎膜增厚，水腫，細(xì)胞增生嚴(yán)重、間質(zhì)增寬。胎膜早破組絨毛膜羊膜炎感染率較對照組明顯升高，，差異有統(tǒng)計學(xué)意義（P＜0.05）；胎膜早破組隨破膜時間的逐漸延長，孕婦WBC計數(shù)明顯升高，差異有統(tǒng)計學(xué)意義（P＜0.05）；4、IV型膠原主要分布于絨毛膜的基底層和羊膜的基底層，IV型膠原免疫組化染色位于細(xì)胞外，呈棕黃色束狀和條索狀；胎膜早破組孕婦胎膜IV型膠原含量平均為160.70，明顯低于對照組，差異有統(tǒng)計學(xué)意義（P＜0.05）。 結(jié)論足月胎膜早破患者母體血漿鋅、血清及羊水MMP-9含量升高、血清IV型膠原含量降低，胎膜IV型膠原表達(dá)降低。鋅、MMP-9、IV型膠原和孕足月胎膜早破有關(guān)。
[Abstract]:Objective to detect the changes of plasma zinc, serum and amniotic fluid MMP-9, serum and amniotic fluid type IV collagen and the expression level of fetal membrane type IV collagen in pregnant women with term premature rupture of membranes, and to analyze the correlation between zinc and amniotic fluid type IV collagen. To explore the relationship between matrix metalloproteinase-9 and its substrate level and term premature rupture of membranes, to provide a theoretical basis for the etiology and prevention of premature rupture of membranes. Methods from November 2012 to March 2013, 30 cases of term premature rupture of membranes were selected as the case group, and 30 cases of non-fetal membrane premature rupture in the same period of term delivery as the control group. The peripheral blood of all the subjects were collected. In amniotic fluid and fetal membrane, plasma zinc content was detected by atomic absorption spectrometry, MMP-9 in serum and amniotic fluid was detected by Elisa, type IV collagen in serum and amniotic fluid was detected by up-conversion luminescence method. The expression of type IV collagen in fetal membrane was detected by immunohistochemical method, and the expression of type IV collagen was observed and scanned by Olympus Bx63 automatic microscope system. The difference of the expression level of the above indexes between the case group and the control group was compared. And the correlation between the indicators were analyzed. Results 1. The plasma zinc content in premature rupture group was significantly higher than that in control group (P < 0.05), and the serum MMP-9 level in premature rupture group was 1463.25 ng / ml, which was significantly higher than that in control group. The average content of MMP-9 in amniotic fluid was 125.48 ng / ml, which was significantly higher than that in the control group (P < 0.05), and the average level of type IV collagen in the pregnant women with premature rupture of membranes was 56.86 ng / ml, which was significantly lower than that in the control group. The content of type IV collagen in amniotic fluid of pregnant women with premature rupture of membranes was significantly higher than that of normal control group (P < 0.05), the difference was statistically significant (P < 0.05). There was a positive correlation between serum MMP-9 and MMP-9 in amniotic fluid (P < 0.05). There was a positive correlation between plasma zinc and serum MMP-9 in pregnant women (P < 0.05), and a negative correlation between plasma zinc and type IV collagen in pregnant women (P < 0.05). There was no correlation between plasma zinc and amniotic fluid MMP-9, serum type IV collagen and serum MMP-9, serum type IV collagen and amniotic fluid MMP-9 (P > 0.05). There was no correlation between maternal plasma zinc, serum type IV collagen and fetal membrane type IV collagen (P > 0.05). There was a negative correlation between MMP-9, MMP-9 in amniotic fluid and type IV collagen of fetal membrane (P < 0.05). In chorioamnionitis, membrane thickening, edema, cell proliferation and interstitial widening were observed. The infection rate of chorioamnionitis in the premature rupture group was significantly higher than that in the control group (P < 0.05), and the WBC count in the premature rupture group was significantly higher than that in the control group (P < 0.05). 4the immunohistochemical staining of type IV collagen mainly distributed in the basal layer of chorion and amniotic membrane was in the form of brown bundles and stripes, and the average content of type IV collagen was 160.70 in the group of premature rupture of membranes, which was significantly lower than that in the control group. The difference was statistically significant (P < 0.05). Conclusion the levels of plasma zinc, MMP-9 in serum and amniotic fluid in the patients with term premature rupture of membranes increased, the level of type IV collagen in serum decreased, and the expression of type IV collagen in membranes decreased. Type IV collagen was associated with premature rupture of membranes in term pregnancy.
【學(xué)位授予單位】：河北聯(lián)合大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.433
本文編號：2133309
[Abstract]:Objective to detect the changes of plasma zinc, serum and amniotic fluid MMP-9, serum and amniotic fluid type IV collagen and the expression level of fetal membrane type IV collagen in pregnant women with term premature rupture of membranes, and to analyze the correlation between zinc and amniotic fluid type IV collagen. To explore the relationship between matrix metalloproteinase-9 and its substrate level and term premature rupture of membranes, to provide a theoretical basis for the etiology and prevention of premature rupture of membranes. Methods from November 2012 to March 2013, 30 cases of term premature rupture of membranes were selected as the case group, and 30 cases of non-fetal membrane premature rupture in the same period of term delivery as the control group. The peripheral blood of all the subjects were collected. In amniotic fluid and fetal membrane, plasma zinc content was detected by atomic absorption spectrometry, MMP-9 in serum and amniotic fluid was detected by Elisa, type IV collagen in serum and amniotic fluid was detected by up-conversion luminescence method. The expression of type IV collagen in fetal membrane was detected by immunohistochemical method, and the expression of type IV collagen was observed and scanned by Olympus Bx63 automatic microscope system. The difference of the expression level of the above indexes between the case group and the control group was compared. And the correlation between the indicators were analyzed. Results 1. The plasma zinc content in premature rupture group was significantly higher than that in control group (P < 0.05), and the serum MMP-9 level in premature rupture group was 1463.25 ng / ml, which was significantly higher than that in control group. The average content of MMP-9 in amniotic fluid was 125.48 ng / ml, which was significantly higher than that in the control group (P < 0.05), and the average level of type IV collagen in the pregnant women with premature rupture of membranes was 56.86 ng / ml, which was significantly lower than that in the control group. The content of type IV collagen in amniotic fluid of pregnant women with premature rupture of membranes was significantly higher than that of normal control group (P < 0.05), the difference was statistically significant (P < 0.05). There was a positive correlation between serum MMP-9 and MMP-9 in amniotic fluid (P < 0.05). There was a positive correlation between plasma zinc and serum MMP-9 in pregnant women (P < 0.05), and a negative correlation between plasma zinc and type IV collagen in pregnant women (P < 0.05). There was no correlation between plasma zinc and amniotic fluid MMP-9, serum type IV collagen and serum MMP-9, serum type IV collagen and amniotic fluid MMP-9 (P > 0.05). There was no correlation between maternal plasma zinc, serum type IV collagen and fetal membrane type IV collagen (P > 0.05). There was a negative correlation between MMP-9, MMP-9 in amniotic fluid and type IV collagen of fetal membrane (P < 0.05). In chorioamnionitis, membrane thickening, edema, cell proliferation and interstitial widening were observed. The infection rate of chorioamnionitis in the premature rupture group was significantly higher than that in the control group (P < 0.05), and the WBC count in the premature rupture group was significantly higher than that in the control group (P < 0.05). 4the immunohistochemical staining of type IV collagen mainly distributed in the basal layer of chorion and amniotic membrane was in the form of brown bundles and stripes, and the average content of type IV collagen was 160.70 in the group of premature rupture of membranes, which was significantly lower than that in the control group. The difference was statistically significant (P < 0.05). Conclusion the levels of plasma zinc, MMP-9 in serum and amniotic fluid in the patients with term premature rupture of membranes increased, the level of type IV collagen in serum decreased, and the expression of type IV collagen in membranes decreased. Type IV collagen was associated with premature rupture of membranes in term pregnancy.
【學(xué)位授予單位】：河北聯(lián)合大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.433
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