【摘要】：目的探討富含酪氨酸重復(fù)序列G蛋白偶聯(lián)受體(leucine-rich repeat-containing G protein-coupled receptor 5,Lgr5)在子宮頸鱗狀細(xì)胞癌(cervical squamous cell carcinoma,CSCC)中的表達(dá)及意義。方法采用免疫組化EnVision兩步法檢測83例CSCC組織、56例癌旁正常組織及32例子宮頸上皮內(nèi)病變(cervical intraepithelial neoplasia,CIN)組織中Lgr5蛋白表達(dá),并應(yīng)用RT-PCR檢測13例CSCC及癌旁組織中Lgr5 mRNA的表達(dá),分析Lgr5表達(dá)與CSCC臨床病理特征的關(guān)系。結(jié)果 Lgr5在CSCC中的表達(dá)明顯高于癌旁正常組織(P0.01)及CIN(P0.05)。Lgr5表達(dá)與腫瘤分化程度(P0.01)和浸潤深度(P0.05)有關(guān),與患者年齡、腫瘤大小、臨床分期及淋巴結(jié)轉(zhuǎn)移無關(guān)(P0.05)。Kaplan-Meier生存分析顯示,Lgr5高表達(dá)者5年生存率顯著低于Lgr5低表達(dá)者(P0.01)。多因素Cox比例風(fēng)險回歸模型分析顯示,Lgr5的表達(dá)(P=0.027)可以作為判斷CSCC患者預(yù)后的獨(dú)立危險因素。結(jié)論 Lgr5的表達(dá)與腫瘤分化程度和浸潤深度密切相關(guān),可能參與CSCC的發(fā)生、發(fā)展,可作為CSCC患者預(yù)后判斷的輔助指標(biāo)。
[Abstract]:Objective to investigate the expression and significance of tyrosine rich repeat G protein-coupled receptor (leucine-rich repeat-containing G protein-coupled receptor 5 Lgr5) in cervical squamous cell carcinoma (cervical squamous cell carcinomonas carcinomas). Methods Immunohistochemical envision method was used to detect the expression of Lgr5 protein in 56 cases of adjacent normal tissues and 32 cases of cervical intraepithelial lesions (cervical intraepithelial neoplasia cin). RT-PCR was used to detect the expression of Lgr5 mRNA in 13 cases of CSCC and adjacent tissues. To analyze the relationship between the expression of Lgr5 and the clinicopathological features of CSCC. Results the expression of Lgr5 in CSCC was significantly higher than that in adjacent normal tissues (P0.01) and cin (P0.05) .Lgr5 expression was correlated with tumor differentiation (P0.01) and depth of invasion (P0.05). There was no correlation between clinical stage and lymph node metastasis (P0.05). Kaplan-Meier survival analysis showed that the 5-year survival rate of patients with high expression of Lgr5 was significantly lower than that of patients with low expression of Lgr5 (P0.01). Multivariate Cox proportional risk regression analysis showed that the expression of Lgr5 (P0. 027) could be used as an independent risk factor for the prognosis of CSCC patients. Conclusion the expression of Lgr5 is closely related to the degree of differentiation and depth of invasion of CSCC and may be involved in the occurrence and development of CSCC. It can be used as an auxiliary index to judge the prognosis of patients with CSCC.
【作者單位】： 浙江省嘉興市中醫(yī)醫(yī)院病理科;
【分類號】：R737.33
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本文編號：2123894