發(fā)布時間：2018-07-07 19:20

  本文選題：KISS基因 + CA　； 參考：《中國腫瘤生物治療雜志》2015年03期

【摘要】：目的:通過與CA125比較,探討外周血KISS1 mRNA在上皮性卵巢癌(epithelial ovarian cancer,EOC)中的診斷價值。方法:采集2010年1月至2014年1月在南通大學第二附屬醫(yī)院住院擬手術的EOC患者40例手術前一天的外周靜脈血和正常健康者靜脈血,用RT-PCR法和電化學發(fā)光法分別檢測外周血KISS1 mRNA和CA125的表達。結(jié)果:EOC早期組(Ⅰ~Ⅱ期)、晚期組(Ⅲ~Ⅳ期)KISS1 mRNA的表達量均顯著高于正常健康者(均P0.01),而早期與晚期EOC組間比較無顯著差異(P0.05);EOC晚期組CA125的表達量明顯高于早期組及正常健康者(均P0.01),而早期組與正常健康者比較無顯著差異(P0.05)。KISS1 mRNA ROC(receptive operator character curve,ROC)曲線的切值(cutoff)設為0.51、0.72時,陽性預測值(positive predictive value,PPV)分別為0.58和1,陰性預測值(negative predictive value,NPV)分別為0.92和0.7;CA125的cutoff值設為20、100 U/ml時,PPV分別為0.72和1,NPV分別為0.87和0.81。KISS1 mRNA、CA125對EOC均具有中度診斷效能(P=0.34);兩者比較,KISS1 mRNA對早期EOC具有較高診斷價值(P=0.018),而CA125對晚期EOC的診斷價值較高(P0.01)。結(jié)論:患者外周血KISS1 mRNA與CA125對EOC具有同等的診斷價值,KISS1 mRNA可作為EOC的一個新的腫瘤標志物;兩者的聯(lián)合檢測可提高EOC的診斷效能,特別是早期EOC的診斷率。
[Abstract]:Objective: to investigate the diagnostic value of KISS1 mRNA in epithelial ovarian cancer (epithelial ovarian cancerEOC) by comparing with CA125. Methods: from January 2010 to January 2014, the peripheral venous blood of 40 EOC patients who were hospitalized in the second affiliated Hospital of Nantong University and the venous blood of healthy people were collected one day before the operation. The expression of KISS1 mRNA and CA125 in peripheral blood were detected by RT-PCR and electrochemiluminescence respectively. Results the expression of KISS1 mRNA in early stage (鈪，

本文編號：2105992