本文選題：宮頸病變 + SCCA��； 參考：《華北理工大學(xué)》2017年碩士論文

【摘要】：目的探討不同宮頸病變組織中鱗狀細(xì)胞癌抗原(SCCA)、腫瘤轉(zhuǎn)移相關(guān)因子-1(MTA1)、多腫瘤抑制基因(P16)及細(xì)胞核相關(guān)抗原(Ki-67)的表達(dá)情況,并分析四種因子與高危型HPV感染的相關(guān)性。方法收集自2015年3月～2016年11月于華北理工大學(xué)附屬醫(yī)院診治患者123例,其中病例組91例,其中包括宮頸低級別上皮內(nèi)病變(low-grade squamous intraepithelial lesions,LSIL)31例、宮頸高級別上皮內(nèi)病變(high-grade squamous intraepithelial lesions,HSIL)31例以及宮頸鱗癌組29例;同時選取慢性宮頸炎組32例作為對照組。所有病理結(jié)果均由2位病理科醫(yī)師證實(shí),且均未采取過放療、化療以及激素治療,收集其臨床資料及其經(jīng)手術(shù)或陰道鏡活檢術(shù)取下的宮頸組織,收集上述病人的宮頸分泌物,采用cobas 4800檢測技術(shù),檢測宮頸分泌物中HPV病毒的DNA,應(yīng)用免疫組化S-P法,檢測不同宮頸組織中SCCA、MTA1、P16及Ki-67的免疫組化染色情況,兩種不同宮頸病變組織中的免疫組化結(jié)果的比較采用秩和檢驗(yàn);采用Western blot方法檢測不同宮頸病變組織中SCCA、MTA1的表達(dá)情況,兩種不同組織間的Western blot結(jié)果的比較用獨(dú)立樣本t檢驗(yàn);并通過斯皮爾曼(Spearman)秩相關(guān)檢驗(yàn)分別分析四種因子與高危型HPV感染的相關(guān)性。結(jié)果1免疫組化結(jié)果顯示,SCCA蛋白在宮頸鱗癌中呈強(qiáng)陽性表達(dá),在HSIL、LSIL中陽性表達(dá),在慢性宮頸炎組表達(dá)較弱,兩兩比較,宮頸鱗癌組織中SCCA的表達(dá)水平和染色范圍均高于HSIL、LSIL組(P0.05);HSIL、LSIL組的表達(dá)高于慢性宮頸炎組(P0.05);HSIL組的表達(dá)量和染色強(qiáng)度高于LSIL,但兩者差異無統(tǒng)計(jì)學(xué)意義(P=0.197);Spearman相關(guān)分析,γ=0.640(雙側(cè)檢驗(yàn)P0.05)。Western blot檢測結(jié)果與免疫組化結(jié)果一致,顯示隨著宮頸病變程度的增加,SCCA蛋白的表達(dá)水平和染色范圍逐漸增加。2免疫組化結(jié)果顯示,MTA1蛋白在宮頸鱗癌及HSIL中呈現(xiàn)出陽性或者是強(qiáng)陽性表達(dá),在LSIL、慢性宮頸炎組表達(dá)較弱,兩兩比較,宮頸鱗癌組織中MTA1的表達(dá)水平和染色范圍高于HSIL、LSIL組,(P0.05);HSIL組均高于LSIL、慢性宮頸炎組(P0.05);LSIL組MTA1的表達(dá)高于慢性宮頸炎組,但兩者比較差異無統(tǒng)計(jì)學(xué)意義(P0.05)。Spearman相關(guān)分析,γ=0.635(雙側(cè)檢驗(yàn)P0.05)。Western blot檢測結(jié)果與免疫組化結(jié)果一致,顯隨著宮頸病變程度的,MTA1蛋白的表達(dá)水平和染色范圍逐漸增加。3 P16蛋白在宮頸鱗癌中呈強(qiáng)陽性表達(dá),在HSIL、LSIL中陽性表達(dá),在慢性宮頸炎組表達(dá)較弱;兩兩比較,宮頸鱗癌組織中P16的表達(dá)水平和染色范圍高于HSIL、LSIL組(P0.05);HSIL、LSIL組高于慢性宮頸炎組(P0.05);Spearman相關(guān)分析,γ=0.784(雙側(cè)檢驗(yàn)P0.05)。顯示隨著宮頸病變程度的,P16蛋白的表達(dá)水平和染色范圍逐漸增加。4 Ki-67蛋白在宮頸鱗癌中強(qiáng)陽性表達(dá),在HSIL、LSIL中陽性表達(dá),在慢性宮頸炎組表達(dá)較弱,兩兩比較,得出宮頸鱗癌組織中Ki-67的表達(dá)高于HSIL、LSIL組(P0.05);HSIL、LSIL組高于慢性宮頸炎組(P0.05)。Spearman相關(guān)分析,γ=0.840(雙側(cè)檢驗(yàn)P0.05);顯示隨著宮頸病變程度的,Ki-67蛋白的表達(dá)水平和染色范圍逐漸增加。5 SCCA的表達(dá)與HR-HPV的感染尚未發(fā)現(xiàn)相關(guān)性(P0.05)。6 MTA1、P16、Ki-67的表達(dá)與HR-HPV的感染呈正相關(guān)(P0.05)。結(jié)論1 SCCA蛋白在宮頸鱗癌中的表達(dá)程度高于HSIL、LSIL組,HSIL、LSIL組高于慢性宮頸炎。2 MTA1蛋白在宮頸鱗癌中的表達(dá)程度高于HSIL組,HSIL組高于LSIL和慢性宮頸炎。3 P16、Ki-67蛋白在宮頸鱗癌中的表達(dá)程度明顯高于HSIL、LSIL組,HSIL、LSIL組高于慢性宮頸炎組。4宮頸組織中SCCA蛋白的表達(dá)程度與HR-HPV的感染尚未發(fā)現(xiàn)相關(guān)性。5宮頸組織中MTA1、P16、Ki-67蛋白的表達(dá)程度與HR-HPV的感染呈正相關(guān)。
[Abstract]:Objective to investigate the expression of squamous cell carcinoma antigen (SCCA), tumor metastasis related factor -1 (MTA1), multiple tumor suppressor gene (P16) and nuclear related antigen (Ki-67) in different cervical lesions, and to analyze the correlation between the four factors and high risk HPV infection. 123 cases were treated in the hospital, of which 91 cases were case group, including 31 cases of low-grade squamous intraepithelial lesions, LSIL, 31 cases of high grade cervical intraepithelial lesion (high-grade squamous intraepithelial lesions, HSIL) and 29 cases of cervical squamous cell carcinoma group, and 32 cases of chronic cervicitis group were selected as control. All the pathological results were confirmed by 2 pathologists, and none had been treated with radiotherapy, chemotherapy and hormone therapy. The clinical data and cervical tissue from the operation or colposcopy biopsy were collected, the cervical secretions of the above patients were collected, the Cobas 4800 technique was used to detect the DNA of the HPV virus in the cervical secretions, and the application of the exemptions was not used. Immuno histochemical staining of SCCA, MTA1, P16 and Ki-67 in different cervical tissues was detected by immunohistochemical S-P method. The immunohistochemical results of two different cervical lesions were compared by the rank sum test, and the Western blot method was used to detect the expression of SCCA, MTA1 in different cervical lesions, and the Western blot results between two different tissues. The independent sample t test was used, and the correlation between the four factors and high risk HPV infection was analyzed by the Spielman (Spearman) rank correlation test. Results 1 immunohistochemical results showed that SCCA protein was strongly positive in cervical squamous cell carcinoma, expressed in HSIL, LSIL, weak in chronic cervicitis group, and 22 in cervical squamous cell scale. The expression level and color range of SCCA in cancer tissues were higher than that in HSIL, group LSIL (P0.05), and the expression of HSIL, LSIL group was higher than that of chronic cervicitis group (P0.05); the expression and intensity of HSIL group were higher than LSIL, but there was no significant difference between them (P=0.197); Spearman correlation analysis, gamma =0.640 (bilateral test) detection results and immunity The results of the histochemistry were consistent. With the increase of the degree of cervical lesions, the expression level of SCCA protein and the staining range increased gradually, and the results of.2 immunohistochemical staining showed that MTA1 protein was positive or strongly positive in cervical squamous cell carcinoma and HSIL. In LSIL, the expression of the chronic cervicitis group was weaker. 22, the expression of MTA1 in cervical squamous cell carcinoma tissue was compared. The level and staining range were higher than that of HSIL, group LSIL, (P0.05), group HSIL was higher than LSIL and chronic cervicitis group (P0.05), and the expression of MTA1 in group LSIL was higher than that of chronic cervicitis group, but there was no significant difference between the two groups (P0.05).Spearman related analysis, and the results of gamma =0.635 (bilateral test P0.05) were in accordance with the immunohistochemical results. The degree of cervical lesions, the expression level of MTA1 protein and the range of staining gradually increased the positive expression of.3 P16 protein in cervical squamous cell carcinoma, positive expression in HSIL, LSIL, and weak expression in chronic cervicitis group. 22, the expression level and color range of P16 in cervical squamous cell carcinoma were higher than that of HSIL, LSIL group (P0.05); HSIL, LSIL group was higher than slow. Sexual cervicitis group (P0.05); Spearman correlation analysis, gamma =0.784 (bilateral test P0.05). With the degree of cervical lesions, the expression level of P16 protein and the range of staining gradually increased the strong positive expression of.4 Ki-67 protein in cervical squamous cell carcinoma, positive expression in HSIL, LSIL, in chronic cervicitis group, 22 comparison, to obtain cervical squamous cell carcinoma. The expression of Ki-67 in tissue was higher than that in HSIL, group LSIL (P0.05), and group HSIL and LSIL was higher than that of chronic cervicitis group (P0.05).Spearman related analysis, and gamma =0.840 (bilateral test P0.05). The expression of P16, Ki-67 was positively correlated with HR-HPV infection (P0.05). Conclusion 1 SCCA protein expression in cervical squamous cell carcinoma is higher than HSIL, LSIL group, HSIL, LSIL group is higher than chronic cervicitis,.2 MTA1 protein expression in cervical squamous cell carcinoma is higher than that of HSIL group. The degree of the SCCA protein in the.4 cervical tissues of the chronic cervicitis group was higher than that of the HSIL, group LSIL, HSIL and LSIL. The degree of SCCA protein expression in the cervical tissues of the chronic cervicitis group was not found to be associated with the HR-HPV infection. The expression of P16, Ki-67 protein was positively correlated with the infection of the HR-HPV.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R737.33

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