本文選題：大黃素衍生物A + 抗轉(zhuǎn)移��； 參考：《中國現(xiàn)代醫(yī)學(xué)雜志》2015年19期

【摘要】：目的研究大黃素衍生物A對(duì)具有不同淋巴結(jié)轉(zhuǎn)移潛能的卵巢癌細(xì)胞的遷移和侵襲能力的抑制作用,并且探討其可能的作用機(jī)制。方法四甲基偶氮唑藍(lán)(MTT)法檢測(cè)不同濃度大黃素衍生物A處理人卵巢漿液性乳頭狀腺癌細(xì)胞(SKOV3)和具有淋巴結(jié)定向高轉(zhuǎn)移能力的亞克隆細(xì)胞(SKOV3-pm4)24 h后細(xì)胞的增殖作用。免疫熒光法觀察細(xì)胞中Rac1蛋白的分布情況。不同濃度藥物作用兩種細(xì)胞后,Western blot檢測(cè)細(xì)胞Rac1蛋白的表達(dá)量,細(xì)胞劃痕實(shí)驗(yàn)檢測(cè)細(xì)胞的遷移能力,Transwell侵襲實(shí)驗(yàn)測(cè)定細(xì)胞的侵襲能力。結(jié)果在一定濃度范圍內(nèi)大黃素衍生物A對(duì)SKOV3和SKOV3-pm4細(xì)胞的增殖均有抑制作用,24 h的半數(shù)抑制濃度(IC50)分別為23.21和34.46 mg/L。免疫熒光觀察到兩種細(xì)胞中Rac1蛋白均廣泛分布于細(xì)胞質(zhì)中,Western blot結(jié)果顯示SKOV3-pm4細(xì)胞Rac1蛋白表達(dá)量高于SKOV3(P0.05)。4和8 mg/L處理組中Rac1蛋白表達(dá)較空白組明顯降低。細(xì)胞劃痕實(shí)驗(yàn)和Transwell小室侵襲實(shí)驗(yàn)結(jié)果顯示,大黃素衍生物A處理后兩種細(xì)胞的遷移和侵襲能力均受到抑制。結(jié)論大黃素衍生物A能抑制具有高淋巴結(jié)轉(zhuǎn)移潛能的卵巢癌細(xì)胞的遷移和侵襲能力,其作用機(jī)制可能與下調(diào)Rac1蛋白的表達(dá)有關(guān)。
[Abstract]:Objective to study the inhibitory effect of emodin derivative A on the migration and invasion ability of ovarian cancer cells with different lymph node metastasis potential, and to explore its possible mechanism. Methods four methyl azazolium (MTT) method was used to detect different concentrations of emodin derivative A in human ovarian serous papillary adenocarcinoma cells (SKOV3) and to have the effect of drenching. The proliferation of subclone cells (SKOV3-pm4) 24 h with high metastasis ability. The distribution of Rac1 protein in the cells was observed by immunofluorescence. After two cells with different concentrations of drugs, Western blot was used to detect the expression of Rac1 protein. Cell scratch test was used to detect cell migration ability and Transwell invasion test. Results in a certain concentration, emodin derivative A could inhibit the proliferation of SKOV3 and SKOV3-pm4 cells. The median inhibitory concentration of 24 h (IC50) was 23.21 and 34.46 mg/L., respectively, and the two cells were found to be widely distributed in the cytoplasm, and Western blot results showed SKOV3-pm4. The expression of Rac1 protein was higher than that in SKOV3 (P0.05).4 and 8 mg/L treatment group, the expression of Rac1 protein was significantly lower than that in the blank group. The cell scratch test and the Transwell chamber invasion experiment showed that the migration and invasion ability of the two cells after the emodin derivative A treatment were inhibited. Conclusion the emodin derivative A can inhibit the high lymph nodes. The migration and invasion of ovarian cancer cells with metastatic potential may be related to the down-regulation of Rac1 protein expression.
【作者單位】： 廣西醫(yī)科大學(xué)醫(yī)學(xué)科學(xué)實(shí)驗(yàn)中心;廣西醫(yī)科大學(xué)附屬腫瘤醫(yī)院實(shí)驗(yàn)研究部;廣西醫(yī)科大學(xué)藥學(xué)院;
【基金】：國家自然基金(No:81060218;81360502) 廣西自然科學(xué)基金(No:2014GXNSFAA118161) 廣西區(qū)域性高發(fā)腫瘤早期防治研究重點(diǎn)實(shí)驗(yàn)室自主研究項(xiàng)目(No:GK2014-ZZ15)
【分類號(hào)】：R737.31

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