本文選題：氧化苦參堿 + SiHa細胞株�。� 參考：《世界科技研究與發(fā)展》2014年03期

【摘要】：目的研究氧化苦參堿對體外人宮頸癌SiHa細胞株增殖活性及凋亡的影響。方法對人宮頸癌SiHa細胞株進行體外培養(yǎng),經(jīng)氧化苦參堿處理的細胞組為實驗組,未經(jīng)處理組為對照組。采用MTT細胞存活實驗檢測氧化苦參堿對入宮頸鱗癌SiHa細胞的增殖影響,并計算IC50;倒置相差顯微鏡下觀察不同濃度的氧化苦參堿作用48h后SiHa細胞的形態(tài)改變;Hoechst33258染色法和Western blot法檢測氧化苦參堿對SiHa細胞核及凋亡相關(guān)蛋白(P53、Bax及Bcl-2)表達水平的影響。結(jié)果 MTT結(jié)果顯示氧化苦參堿劑量-時間依耐性抑制人宮頸癌SiHa細胞的體外增殖(P0.05),計算24 h、48 h和72 h的IC50分別為(1 028.41±3.57)μg/ml、(701.72±6.01)μg/ml和(406.88±2.15)μg/ml;氧化苦參堿處理48 h后,SiHa細胞的形態(tài)特征及數(shù)目發(fā)生顯著變化,且隨氧化苦參堿濃度的增加而愈加明顯;Hoechst33258染色證實氧化苦參堿處理后SiHa細胞發(fā)生凋亡,可見典型的凋亡特征;Western blot結(jié)果證實氧化苦參堿(700μg/ml、1 600μg/ml)處理48 h后,和對照組比較,藥物處理組細胞凋亡周期蛋白P53、Bax表達上調(diào)(P0.05),而Bcl-2表達下調(diào)(P0.05),Bax/Bcl-2的比率明顯增加(P0.05)。結(jié)論氧化苦參堿可抑制體外人宮頸癌SiHa細胞的體外增殖活性發(fā)揮其抗腫瘤作用,其作用機制可能誘導(dǎo)SiHa細胞的凋亡有關(guān)。
[Abstract]:Objective to study the effect of oxymatrine on proliferation and apoptosis of human cervical cancer cell line SIHA in vitro. Methods Human cervical cancer SIHA cells were cultured in vitro. The cells treated with oxymatrine were treated as experimental group and untreated group as control group. MTT cell survival assay was used to detect the effect of oxymatrine on the proliferation of SIHA cells in cervical squamous cell carcinoma. The morphologic changes of SIHA cells treated with different concentrations of oxymatrine for 48 h were observed. Hoechst33258 staining and Western blot were used to detect the effect of oxymatrine on the expression of nuclear and apoptosis-related proteins (P53, Bax and Bcl-2) in SIHA cells. Results MTT results showed that oxymatrine inhibited the proliferation of human cervical cancer SIHA cells in vitro according to tolerance (P0.05). The IC50 of SIHA cells at 24 h for 48 h and 72 h were (1 028.41 鹵3.57) 渭 g / ml, (701.72 鹵6.01) 渭 g/ml and (406.88 鹵2.15) 渭 g / ml, respectively. Signs and numbers have changed significantly, With the increase of oxymatrine concentration, Hoechst33258 staining proved that apoptosis occurred in SIHA cells after treated with oxymatrine. The typical apoptotic characteristics of SIHA cells were confirmed by Western blot. The results showed that Oxymatrine (700 渭 g / ml, 1 600 渭 g/ml) was treated for 48 h, compared with the control group. In drug treated group, the expression of apoptosis cyclin P53 + Bax was up-regulated (P0.05), while the expression of Bcl-2 was down-regulated (P0.05) and the ratio of Bax / Bax was significantly increased (P0.05). Conclusion Oxymatrine can inhibit the proliferation activity of human cervical carcinoma SIHA cells in vitro and play an antitumor role, and its mechanism may be related to the apoptosis of SIHA cells.
【作者單位】： 蘭州大學(xué)第二臨床醫(yī)學(xué)院;蘭州大學(xué)第二醫(yī)院婦科;上海市浦東新區(qū)周浦醫(yī)院;
【分類號】：R273

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