本文選題：質(zhì)膜修復(fù)蛋白 + 半胱氨酸天冬氨酸蛋白酶��； 參考：《南昌大學(xué)》2014年碩士論文

【摘要】：目的：利用組織學(xué)觀察妊娠期高血壓疾病患者胎盤的形態(tài)學(xué)特征，利用免疫組織化學(xué)SP法研究質(zhì)膜修復(fù)蛋白（dysferlin）及半胱氨酸天冬氨酸蛋白酶-3（caspase-3）在胎盤的表達(dá)，以探討其與妊娠期高血壓疾病發(fā)病的關(guān)系。 方法：隨機(jī)選擇正常妊娠患者45例，妊娠期高血壓患者15例，輕度子癇前期患者15例，重度子癇前期患者15例，制作光鏡切片，進(jìn)行常規(guī)HE染色，觀察各組胎盤的形態(tài)學(xué)改變。借助免疫組織化學(xué)SP法，應(yīng)用dysferlin及caspase-3抗體，定位dysferlin和casapse-3在胎盤的表達(dá)及其在各組的表達(dá)差異。 結(jié)果：1、光鏡下觀察常規(guī)HE切片：正常胎盤的合體滋養(yǎng)細(xì)胞位于絨毛表層，細(xì)胞核排列較整齊，細(xì)胞結(jié)構(gòu)一致。妊娠期高血壓患者胎盤的滋養(yǎng)細(xì)胞增多，可見閉塞性血管內(nèi)膜炎、絨毛血管灌注減少，纖維蛋白樣壞死絨毛增多、合體結(jié)增多。 2、Dysferlin在各組的胎盤中均有表達(dá)，主要分布于胎盤合體滋養(yǎng)細(xì)胞膜。實(shí)驗(yàn)組的Dysferlin在合體滋養(yǎng)細(xì)胞膜的表達(dá)強(qiáng)度低于對(duì)照組，，重度子癇前期組（2.07±1.83）、輕度子癇前期組（4.67±1.95）與對(duì)照組（6.64±2.14）相比，存在顯著差異（P0.05），妊娠期高血壓組（5.60±1.45）與對(duì)照組相比無顯著差異（P0.05）。 3、Caspase-3在各組的胎盤中均有表達(dá)，主要分布于胎盤合體滋養(yǎng)細(xì)胞漿及部分細(xì)胞核。實(shí)驗(yàn)組的Caspase-3在合體滋養(yǎng)細(xì)胞膜的表達(dá)強(qiáng)度高于對(duì)照組，重度子癇前期組（7.20±1.74）、輕度子癇前期組（5.93±1.28）和妊娠期高血壓組（5.33±1.45）與對(duì)照組（2.64±2.25）相比，存在顯著差異（P0.05）。 4、Dysferlin和Caspase-3在胎盤中表達(dá)呈負(fù)相關(guān)，相關(guān)系數(shù)r=-0.4167（P0.001）。 結(jié)論：1、Dysferlin在胎盤的合體滋養(yǎng)細(xì)胞膜表達(dá)，并隨著妊娠期高血壓疾病病情的加重表達(dá)強(qiáng)度減弱。 2、Caspase-3在胎盤的合體滋養(yǎng)細(xì)胞的細(xì)胞漿及細(xì)胞核表達(dá)，并隨著妊娠期高血壓疾病病情的加重表達(dá)強(qiáng)度增強(qiáng)。 3、Dysferlin和Caspase-3在胎盤的合體滋養(yǎng)細(xì)胞的表達(dá)呈負(fù)相關(guān)性，兩者的相互影響可能與妊娠期高血壓疾病的發(fā)生發(fā)展有關(guān)。
[Abstract]:Objective: To observe the morphological characteristics of placenta in patients with pregnancy induced hypertension, and to study the expression of plasmalemma repair protein (dysferlin) and cysteine aspartic proteinase -3 (caspase-3) in placenta by immunohistochemical SP method in order to explore the relationship between the placenta and the pathogenesis of hypertensive disorder complicating pregnancy.
Methods: 45 cases of normal pregnancy, 15 cases of pregnancy induced hypertension, 15 cases of mild preeclampsia, 15 cases of severe preeclampsia, 15 cases of severe preeclampsia were made by light microscopy, routine HE staining was made to observe the morphological changes of the placenta in each group. With the help of immunohistochemical SP method, dysferlin and caspase-3 antibody were used to locate dysferlin and CASAP. The expression of se-3 in placenta and its difference in each group.
Results: 1, the normal HE sections were observed under light microscope: the normal placental syncytiotrophoblast was located in the surface of the villi, the nuclei arranged neatly and the cell structure was the same. The placental trophoblast cells of the patients with pregnancy induced hypertension were increased, the obliterans endocarditis, the decrease of villi blood vessel perfusion, the increase of fibrin like necrosis, and the increase of chimerism.
2, Dysferlin was expressed in all groups of placenta, mainly distributed in the placental syncytial cell membrane. The expression of Dysferlin in the experimental group was lower than that of the control group, the severe preeclampsia group (2.07 + 1.83), and the mild preeclampsia group (4.67 + 1.95) compared with the group (6.64 + 2.14), there were significant differences (P0.05), pregnancy period. The hypertension group (5.60 + 1.45) had no significant difference compared with the control group (P0.05).
3, Caspase-3 was expressed in all groups of placenta, mainly distributed in the placental syncytial trophoblast pulp and part of the nucleus. The expression of Caspase-3 in the experimental group was higher than that of the control group, the severe preeclampsia group (7.20 + 1.74), the mild preeclampsia group (5.93 + 1.28) and the pregnancy induced hypertension group (5.33 + 1.45) and the control group (2 There were significant differences in.64 + 2.25 (P0.05).
4, there was a negative correlation between Dysferlin and Caspase-3 expression in placenta, and the correlation coefficient r=-0.4167 (P0.001).
Conclusion: 1, Dysferlin is expressed in the syncytial trophoblast membrane of placenta and weakened with the aggravation of the condition of hypertensive disorder complicating pregnancy.
2, the expression of Caspase-3 in the cytoplasm and nucleus of placental syncytial trophoblastic cells increased with the aggravation of the condition of hypertensive disorder complicating pregnancy.
3, the expression of Dysferlin and Caspase-3 in the placental chimeric trophoblast is negatively correlated, and the interaction between them may be related to the occurrence and development of hypertensive disorder in pregnancy.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714.246

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉艷慧;黃亞絹;;合體滋養(yǎng)層細(xì)胞微粒與子癇前期的關(guān)系[J];中國(guó)優(yōu)生與遺傳雜志;2012年01期

2 劉艷慧;黃亞絹;;合體滋養(yǎng)層細(xì)胞微粒在子癇前期重度發(fā)病中的作用[J];山東醫(yī)藥;2011年40期

3 馬惠靜;楊海瀾;馮敏;王海燕;索明莉;;Survivin、Caspase-3在子癇前期患者胎盤組織中的表達(dá)及意義[J];中國(guó)婦幼保健;2011年20期

4 岳原亦;張揚(yáng);張一奇;;Caspase家族與細(xì)胞凋亡[J];中國(guó)醫(yī)療前沿;2011年06期

5 趙瑞杰;李引乾;王會(huì);王廣彬;娜日蘇;金大鵬;關(guān)偉軍;馬月輝;;Caspase家族與細(xì)胞凋亡的關(guān)系[J];中國(guó)畜牧雜志;2010年17期

6 戴昕;李占全;冀林華;;凋亡相關(guān)蛋白Caspase研究進(jìn)展[J];中國(guó)現(xiàn)代醫(yī)藥雜志;2010年04期

7 黃小玲;王自能;;Dysferlin在子癇前期胎盤中的表達(dá)[J];實(shí)用醫(yī)學(xué)雜志;2009年11期

8 王悅;;妊娠期高血壓疾病20例胎盤病理學(xué)改變分析[J];中國(guó)誤診學(xué)雜志;2007年08期

9 李樺;馬玉燕;宋佳倫;馬麗娟;傅慶詔;;正常妊娠和子癇前期患者外周血樹突細(xì)胞亞群的變化[J];現(xiàn)代婦產(chǎn)科進(jìn)展;2007年03期

10 王玉;孫黎光;夏春輝;;Caspase介導(dǎo)的Fas凋亡途徑[J];世界華人消化雜志;2006年36期

本文編號(hào)：2025064