本文選題：SALL + 卵巢癌　； 參考：《中華腫瘤防治雜志》2017年07期

【摘要】：目的婆羅雙樹樣基因2(sal-like gene 2,SALL2)作為腫瘤抑制基因,在多種腫瘤細胞的生長過程中發(fā)揮調(diào)控作用。本研究旨在探討SALL2基因?qū)β殉舶?ovarian cancer,OC)A2780細胞生長和轉(zhuǎn)移的影響。方法采用小干擾RNA(small-interfering ribonucleic acid,siRNA)轉(zhuǎn)染OC A2780細胞以沉默SALL2,設載體組及空白對照組。采用RT-PCR和蛋白質(zhì)印跡法檢測SALL2的表達。采用CCK-8和流式細胞術(shù)(flow cytometry,FCM)檢測細胞增殖,采用FCM檢測細胞凋亡。應用劃痕實驗檢測細胞遷移,采用侵襲實驗檢測細胞侵襲。結(jié)果 A2780細胞高表達SALL2的mRNA及蛋白。RT-PCR結(jié)果顯示,轉(zhuǎn)染siRNA2和siRNA3 48h后,空白對照組、載體組、siRNA2和siRNA3組平均2-ΔΔCt值分別為1.000±0.030、0.942±0.053、0.207±0.041和0.465±0.007,差異有統(tǒng)計學意義,F=8.213,P=0.024。轉(zhuǎn)染siRNA2和siRNA3 48h后,空白對照組、載體組及siRNA2和siRNA3組蛋白表達相對灰度比值分別為0.629±0.035、0.603±0.072、0.225±0.004和0.453±0.061,差異有統(tǒng)計學意義,F=11.629,P=0.018。siRNA2組轉(zhuǎn)染72h后的細胞增殖力(4.285±0.026)顯著高于載體組(3.622±0.029)和空白對照組(3.614±0.016),差異有統(tǒng)計學意義,F=34.023,P=0.012。siRNA2組轉(zhuǎn)染48h后的細胞凋亡率為(49.17±2.03)%,顯著低于載體組的(56.82±2.74)%和空白對照組的(58.39±2.45)%,差異有統(tǒng)計學意義,F=5.781,P=0.037。siRNA2組轉(zhuǎn)染48h后處于G0/G1期的細胞比例為(47.87±1.28)%,均顯著低于載體組的(55.27±1.46)%與空白對照組的(56.60±1.57)%,差異有統(tǒng)計學意義,F=4.213,P=0.032。siRNA2組轉(zhuǎn)染48h后劃痕愈合率為(78.925±6.133)%,明顯高于載體組的(38.504±3.772)%和空白對照組的(42.169±4.103)%,差異有統(tǒng)計學意義,F=17.184,P=0.006。siRNA2組轉(zhuǎn)染48h后穿過基質(zhì)膠的細胞數(shù)(147.169±7.208)顯著高于載體組(92.169±11.052)和空白對照組(95.169±9.830),差異有統(tǒng)計學意義,F=12.698,P=0.014。結(jié)論 SALL2沉默促進A2780細胞增殖、遷移和侵襲,抑制細胞凋亡。
[Abstract]:Objective as a tumor suppressor gene, 2(sal-like gene _ 2 and SALL2) play a regulatory role in the growth of various tumor cells. The aim of this study was to investigate the effect of SALL2 gene on the growth and metastasis of ovarian cancer cell line OCCAN A2780. Methods OC-A2780 cells were transfected with small interfering ribonucleic siRNAs to silence SALL2. Vector group and blank control group were set up. The expression of SALL2 was detected by RT-PCR and Western blotting. CCK-8 and flow cytometric FCM were used to detect cell proliferation and FCM to detect cell apoptosis. Scratch test was used to detect cell migration and invasion test was used to detect cell invasion. Results after transfection of siRNA2 and siRNA3 for 348h, the average values of 2- 螖 Ct in control group, vector group and siRNA3 group were 1.000 鹵0.030, 0.942 鹵0.053, 0.207 鹵0.041 and 0.465 鹵0.007, respectively. The difference was statistically significant. After transfection of siRNA2 and siRNA3 for 48 h, the control group was treated with siRNA2 and siRNA3. The relative gray ratio of protein expression in vector group, siRNA2 and siRNA3 group was 0.629 鹵0.035 鹵0.072 鹵0.225 鹵0.004 and 0.453 鹵0.061, respectively. The difference was statistically significant (P < 0.01). The proliferative power of the vector group was significantly higher than that of the vector group (3.622 鹵0.026) and the blank control group (3.614 鹵0.016), and the difference was statistically significant. After 48 h transfection, the cell apoptosis rate was 49.17 鹵2.03g, which was significantly lower than that in the vector group (56.82 鹵2.74%) and the blank control group (58.39 鹵2.45%). The difference was statistically significant (P < 0.01). The percentage of cells in the G _ 0 / R _ 1 phase of the control group was 47.87 鹵1.28%, which was significantly lower than that of the vector group (55.27 鹵1.46g%) and the blank control group (P < 0.05). 56.60 鹵1.57, the difference was statistically significant (P < 0.05). The rate of scratch healing in group 2 was 78.925 鹵6.133 after transfection for 48 h, which was significantly higher than that in vector group (38.504 鹵3.772%) and blank control group (42.169 鹵4.103%). The difference was statistically significant. The number of cells passing through the matrix gel in group 2 was significantly higher than that in control group (147.169 鹵7.208). The difference between the control group (92.169 鹵11.052) and the blank control group (95.169 鹵9.830) was statistically significant (P < 0.05). Conclusion SALL2 silencing can promote the proliferation, migration and invasion of A2780 cells and inhibit the apoptosis of A2780 cells.
【作者單位】： 濱州醫(yī)學院免疫學教研室;
【基金】：國家自然科學基金(81001300)
【分類號】：R737.31
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本文編號：2012672