本文選題：miRNA-21 + miRNA-218��； 參考：《廣西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：探討miR-21、miR-218在宮頸癌及癌前組織中的表達(dá)及與高危型HPV感染和宮頸癌臨床病理特征的關(guān)系。方法：（1）利用miRNA芯片技術(shù)篩查宮頸癌、宮頸上皮內(nèi)瘤變中差異表達(dá)的miRNA，并用實時定量RT-PCR在108例宮頸組織標(biāo)本中對miR-21、miR-218進(jìn)行驗證。（2）采用巢式多重PCR（NMPCR）技術(shù)對宮頸癌及癌前病變組織進(jìn)行HPV分型檢測。（3）利用統(tǒng)計學(xué)方法分析miR-21、miR-218在宮頸癌組織中的表達(dá)與高危型HPV感染及臨床病理的關(guān)系。結(jié)果：（1）芯片結(jié)果顯示宮頸癌及高級別鱗狀上皮內(nèi)病變（HSIL）組織中存在差異表達(dá)的miRNAs，，其中miR-218顯示為最明顯的下調(diào)，miR-21顯示為最明顯的上調(diào)，經(jīng)實時定量RT-PCR驗證結(jié)果一致。（2）宮頸癌及高級別鱗狀上皮內(nèi)病變中高危型HPV感染陽性率較高，均大于72%，以HPV-16感染為主，其次為HPV-18,HPV-45；高危型HPV感染與宮頸癌病理類型無明顯相關(guān)性。（3）miR-21的表達(dá)與宮頸癌組織分化程度及高危型HPV感染有關(guān)，但與宮頸癌的FIGO分期、病理類型、淋巴結(jié)轉(zhuǎn)移、脈管浸潤等臨床病理特征之間無明顯相關(guān)性。（4）miR-218的表達(dá)與高危型HPV感染密切相關(guān)，但與宮頸癌的FIGO分期、病理類型、組織分化、淋巴結(jié)轉(zhuǎn)移、脈管浸潤等臨床病理特征之間無明顯相關(guān)性。結(jié)論：miR-21在宮頸癌組織中存在異常高表達(dá)，且與宮頸癌組織分化程度、高危型HPV感染相關(guān)；而miR-218在宮頸癌及癌前組織中則存在異常低表達(dá)，且與高危型HPV感染密切相關(guān)；miR-21、miR-218可能在宮頸癌及癌前病變中其起重要作用。
[Abstract]:Objective: to investigate the expression of miR-21 miR-218 in cervical carcinoma and precancerous tissues and its relationship with high-risk HPV infection and clinicopathological features of cervical cancer. Methods MiRNA microarray was used to screen cervical cancer. The differential expression of miRNAs in cervical intraepithelial neoplasia and the verification of miR-21 miR-218 in 108 cervical tissues by real-time quantitative RT-PCR were used to detect the HPV typing of cervical carcinoma and precancerous lesions by nested multiplex PCRNMPCRR). Methods the relationship between the expression of miR-21 miR-218 and high risk HPV infection and clinicopathology was analyzed. Results the microarray showed that there were differentially expressed miRNAss in cervical carcinoma and high grade squamous intraepithelial lesions (HSILs), in which miR-218 showed the most obvious down-regulation of miR-21 and the most obvious up-regulation of miR-21. The positive rate of high risk HPV infection in cervical carcinoma and high grade squamous intraepithelial lesions was higher than that in 722 cases. HPV-16 infection was the main infection. There was no significant correlation between high risk HPV infection and the pathological type of cervical cancer. The expression of miR-21 was related to the degree of cervical cancer differentiation and high risk HPV infection, but it was associated with Figo stage, pathological type and lymph node metastasis of cervical cancer. There was no significant correlation between vascular invasion and other clinicopathological features. The expression of miR-218 was closely related to high-risk HPV infection, but it was associated with Figo stage, pathological type, tissue differentiation, lymph node metastasis of cervical cancer. There was no significant correlation between the clinicopathological features such as vascular infiltration. Conclusion there is abnormal high expression of miR-21 in cervical carcinoma, which is related to the degree of differentiation and high risk HPV infection, but miR-218 is very low in cervical cancer and precancerous tissue. MiR-21 miR-218 may play an important role in cervical cancer and precancerous lesions.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.33

【參考文獻(xiàn)】

相關(guān)期刊論文 前9條

1 ;New insight into the role of miRNAs in leukemia[J];Science in China(Series C:Life Sciences);2009年03期

2 陳文t

本文編號：1997262