本文選題：卵巢生殖干細(xì)胞 + 化療�。� 參考：《中國婦幼保健》2016年17期

【摘要】：目的探索小鼠卵巢生殖干細(xì)胞(OSCs)卵巢內(nèi)移植是否可以改善化療誘導(dǎo)的卵巢損傷。方法采用腹腔注射環(huán)磷酰胺和白消安建立化療誘導(dǎo)的小鼠卵巢損傷模型,并隨機(jī)分為模型組及移植組,同齡小鼠作為正常對(duì)照組。移植組在建模1周后,雙側(cè)卵巢分別移植OSCs。移植后觀察小鼠動(dòng)情周期;檢測血清雌二醇(E2)和卵泡刺激素(FSH)濃度;測量卵巢重量及直徑;卵巢組織切片觀察卵巢結(jié)構(gòu)和卵泡數(shù)目。結(jié)果 90%的模型組小鼠動(dòng)情周期紊亂,70%移植組小鼠恢復(fù)正常的動(dòng)情周期。移植組E2平均濃度(55.8±6.342)pg/ml高于模型組(34.1±4.643)pg/ml,差異有統(tǒng)計(jì)學(xué)意義(t=6.758,P0.05),而FSH濃度(28.3±1.433)ng/ml低于模型組(64.9±5.553)ng/ml,差異有統(tǒng)計(jì)學(xué)意義(t=-15.631,P0.05)。模型組卵巢呈萎縮狀態(tài),而移植組卵巢得到明顯改善,形態(tài)、大小和正常對(duì)照組相似。移植組卵巢內(nèi)各級(jí)卵泡數(shù)目均高于模型組,差異有統(tǒng)計(jì)學(xué)意義(t=11.851,P0.05),但仍低于正常對(duì)照組,差異亦有統(tǒng)計(jì)學(xué)意義(t=6.505,P0.05)。結(jié)論卵巢生殖干細(xì)胞卵巢內(nèi)移植可以部分地修復(fù)化療導(dǎo)致的卵巢損傷,從而改善卵巢功能。
[Abstract]:Objective to investigate whether intraovarian transplantation of mouse ovarian reproductive stem cell (OSCs) can improve ovarian injury induced by chemotherapy. Methods the ovarian injury model of mice induced by chemotherapy was established by intraperitoneal injection of cyclophosphamide and oxacol. The mice were randomly divided into model group and transplantation group, and the same age mice were used as normal control group. OSCs were transplanted into bilateral ovaries 1 week later in the transplantation group. After transplantation, the estrous cycle of mice was observed, serum estradiol E2) and follicle stimulating hormone (FSH) concentration were detected, ovarian weight and diameter were measured, ovarian structure and follicle number were observed in ovarian tissue sections. Results 90% of the mice in the model group had abnormal estrous cycle and 70% of the transplanted mice returned to normal estrous cycle. The average E _ 2 concentration in the transplantation group was 55.8 鹵6.342)pg/ml higher than that in the model group (34.1 鹵4.643) PG / ml, the difference was statistically significant (P < 0.05), while the concentration of FSH was 28.3 鹵1.433)ng/ml lower than that in the model group (64.9 鹵5.553ng / ml). The difference was statistically significant (t -15.631P 0.05). The model group showed atrophy, while the transplanted group got obvious improvement, shape, size and normal control group were similar. The number of ovarian follicles in the transplanted group was higher than that in the model group, and the difference was statistically significant (P 0.05), but still lower than that in the normal control group. The difference was also statistically significant. Conclusion Intraovarian transplantation of ovarian reproductive stem cells can partially repair ovarian damage induced by chemotherapy and improve ovarian function.
【作者單位】： 華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬同濟(jì)醫(yī)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(81370469) 科技部國家國際科技合作專項(xiàng)項(xiàng)目(No.2013DFA31400)
【分類號(hào)】：R711.75;R730.53

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