本文選題：轉(zhuǎn)化生長(zhǎng)因子-β + 人卵巢癌細(xì)胞系��； 參考：《山東醫(yī)藥》2017年31期

【摘要】：目的觀察轉(zhuǎn)化生長(zhǎng)因子-β(TGF-β)對(duì)人卵巢癌細(xì)胞系SK-OV-3活性氧簇(ROS)表達(dá)的影響,并探討其可能機(jī)制。方法取對(duì)數(shù)生長(zhǎng)期SK-OV-3細(xì)胞,隨機(jī)分為兩組,觀察組用2 mL培養(yǎng)液+2μL無(wú)血清DMEM配制的5μg/mL TGF-β培養(yǎng)(最終濃度為5 ng/mL),對(duì)照組直接加2 mL培養(yǎng)液。流式細(xì)胞儀檢測(cè)細(xì)胞內(nèi)總的活性氧簇(ROS)產(chǎn)生及線粒體來(lái)源的ROS,實(shí)時(shí)熒光定量PCR檢測(cè)細(xì)胞上皮細(xì)胞-間充質(zhì)轉(zhuǎn)化(EMT)變化各指標(biāo)、線粒體呼吸鏈復(fù)合體Ⅲ組分mRNA。結(jié)果觀察組及對(duì)照組總ROS產(chǎn)生量分別為3 159±42.92、1 062±24.41,兩組比較,P0.05。觀察組及對(duì)照組細(xì)胞內(nèi)線粒體來(lái)源ROS產(chǎn)生量分別為1 254±29.04、1 039±17.32,兩組比較,P0.05。觀察組E-cadherin、N-cadherin、Vimentin、ZEB1分別是對(duì)照組的0.200 0、6.739 8、4.487 9、1.639 5倍,P均0.05。觀察組CYC1、TTC19、UQCRC1、UQCRC2、UQCR10、UQCRB、UQCRFS1、UQCRH、UQCRQ mRNA表達(dá)水平較對(duì)照組改變倍數(shù)分別為0.89、1.04、1.09、0.87、1.17、1.18、0.92、1.09、1.07倍,P均0.05。觀察組CYC1、TTC19、UQCRC1、UQCRC2、UQCRFS1蛋白水平較對(duì)照組改變倍數(shù)分別為1.01、0.97、1.07、0.99、1.03倍,P均0.05。結(jié)論TGF-β誘導(dǎo)細(xì)胞內(nèi)總的ROS表達(dá)增加,但過(guò)量ROS并非來(lái)源于線粒體,機(jī)制可能是通過(guò)調(diào)控細(xì)胞內(nèi)氧化-抗氧化平衡系統(tǒng)的關(guān)鍵酶從而改變細(xì)胞內(nèi)的ROS水平。
[Abstract]:Objective to investigate the effect of transforming growth factor- 尾 (TGF- 尾) on the expression of reactive oxygen species (Ros) in human ovarian cancer cell line SK-OV-3, and to explore its possible mechanism. Methods SK-OV-3 cells in logarithmic growth phase were randomly divided into two groups. The experimental group was cultured with 5 渭 g/mL TGF- 尾 (final concentration was 5 ng / mL) with 2 渭 L serum-free DMEM (the final concentration was 5 ng / mL), and the control group was directly supplemented with 2 mL culture medium. The total reactive oxygen species (Ros) production and mitochondrial origin were detected by flow cytometry, the changes of EMTT in epithelial cells and mesenchymal transformation were detected by real-time fluorescence quantitative PCR, and the mitochondrial respiratory chain complex 鈪，

本文編號(hào)：1973755