發(fā)布時間：2018-06-01 16:29

  本文選題：體外受精 + 11β-HSD1　； 參考：《浙江大學(xué)》2014年碩士論文

【摘要】：目的：研究IVF雌性小鼠骨骼肌基因11β-HSD1H6pd及Nr3cl的表達(dá),探索IVF-ET子代成年后代謝性疾病發(fā)生的可能機(jī)制。 材料和方法： 1.利用C57BL/6J小鼠建立IVF及體內(nèi)受精小鼠模型。 2.以IVF子代為研究對象,以體內(nèi)受精子代為對照組,于3周(斷乳期)、8周(性成熟期)、1.5年(老年期)取雌性小鼠骨骼肌。 3.通過qRT-PCR技術(shù),檢測3周、8周及1.5年齡時期雌性小鼠骨骼肌基因11β-HSD1H6pd及Nr3c1的表達(dá)。 4.通過western-blot技術(shù),檢測3周、8周及1.5年齡時期雌性小鼠骨骼肌上11β-HSD1蛋白的表達(dá)。 結(jié)果： 1.獲IVF小鼠15只,體內(nèi)受精小鼠15只。 2.IVF對出生后子代骨骼肌11β-HSD1H6pd及Nr3cl基因表達(dá)的影響 (1) qRT-PCR分析結(jié)果表明,IVF組小鼠子代與對照組之間骨骼肌組織上的11β-HSD1及H6pd基因表達(dá)從3周齡至老年期逐步出現(xiàn)差異,但兩組間Nr3c1的表達(dá)各時期均無顯著性差異。 (2)3周齡時11β-HSD1和H6pd的表達(dá)在兩組之間差異無統(tǒng)計學(xué)意義；8周齡時,IVF組小鼠骨骼肌組織H6pd的表達(dá)顯著性高于體內(nèi)受精組,11β-HSD1的表達(dá)在兩組之間無顯著性差異；1.5年齡時,IVF組小鼠骨骼肌組織11β-HSD1和H6pd表達(dá)水平均較體內(nèi)受精組顯著性升高。Nr3c1的表達(dá)從3周齡至1.5年齡,兩組間均無顯著性差異。 3. IVF對出生后子代骨骼肌11β-HSD1蛋白表達(dá)的影響骨骼肌11β-HSD1蛋白的表達(dá)在兩組間差異無統(tǒng)計學(xué)意義。 結(jié)論： 1. IVF技術(shù)可導(dǎo)致雌性小鼠子代骨骼肌的11β-HSD1和H6PD基因表達(dá)的改變。 2. IVF技術(shù)不影響雌性小鼠骨骼肌上的Nr3c1基因表達(dá)。
[Abstract]:Aim: to study the expression of 11 尾 -HSD1H6pd and Nr3cl in skeletal muscle of female IVF mice and to explore the possible mechanism of metabolic disease in adult IVF-ET offspring. Materials and methods: 1. The model of IVF and in vivo fertilization mice was established by using C57BL/6J mice. 2. The female skeletal muscles of female mice were collected from IVF offspring and spermatozoa in vivo as control group at 3 weeks (weaning stage 8 weeks (sexual maturity stage, 1.5 years old age). 3. The expression of skeletal muscle gene 11 尾 -HSD1H6pd and Nr3c1 in female mice at 3 weeks, 8 weeks and 1.5 years was detected by qRT-PCR technique. 4. The expression of 11 尾 -HSD1 protein on skeletal muscle of female mice at 3 weeks, 8 weeks and 1.5 years was detected by western-blot technique. Results: 1. Fifteen IVF mice and 15 fertilized mice in vivo were obtained. Effect of 2.IVF on expression of 11 尾 -HSD1H6PD and Nr3cl genes in skeletal muscle of postnatal generation The results of qRT-PCR analysis showed that the expression of 11 尾 -HSD1 and H6pd genes in skeletal muscle tissues of mice in the control group and in the control group was gradually different from 3 weeks to the old age, but there was no significant difference in the expression of Nr3c1 between the two groups at all stages. There was no significant difference between the two groups in the expression of 11 尾 -HSD1 and H6pd at the age of 3 weeks. The expression of H6pd in the skeletal muscle of the mice in the 8-week old group was significantly higher than that in the in vivo fertilization group. There was no significant difference between the two groups in the expression of 11 尾 -HSD1 and H6pd. The expression levels of 11 尾 -HSD1 and H6pd in skeletal muscle of mice in the control group were significantly higher than those in the in vivo fertilization group. The expression of Nr3c1 was from 3 weeks to 1.5 years old. There was no significant difference between the two groups. 3. Effect of IVF on the expression of 11 尾 -HSD1 protein in skeletal muscle after birth there was no significant difference between the two groups in the expression of 11 尾 -HSD1 protein in skeletal muscle. Conclusion: 1. The expression of 11 尾 -HSD1 and H6PD genes in skeletal muscle of female mice could be changed by IVF technique. 2. IVF technique did not affect the expression of Nr3c1 gene in skeletal muscle of female mice.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R714.8

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