天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

孕鼠鐵缺乏對(duì)子代GH-IGF-1軸的影響

發(fā)布時(shí)間:2018-05-30 00:13

  本文選題:鐵缺乏 + 生長(zhǎng)激素 ; 參考:《中國(guó)現(xiàn)代醫(yī)學(xué)雜志》2017年08期


【摘要】:目的探討大鼠妊娠期鐵缺乏對(duì)新生仔鼠生長(zhǎng)激素-胰島素樣生長(zhǎng)因子-1(GH-IGF-1)軸的影響及機(jī)制。方法 4周齡雌性Wistar大鼠36只,隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組,分別飼低鐵飼料和含鐵飼料喂養(yǎng)4周,檢測(cè)雌鼠血紅蛋白(Hb)濃度,血細(xì)胞比容(HCT)、紅細(xì)胞平均容積(MCV)、紅細(xì)胞平均血紅蛋白量(MCH)、紅細(xì)胞平均血紅蛋白濃度(MCHC)及紅細(xì)胞分布寬度(RDW)等血液學(xué)相關(guān)參數(shù)。將雌鼠與正常雄鼠合籠,孕末期檢測(cè)血液學(xué)相關(guān)參數(shù)并行骨髓鐵染色。觀察仔鼠存活率及體重。實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)(qRTPCR)方法檢測(cè)新生仔鼠垂體生長(zhǎng)激素(GH)及肝臟胰島素樣生長(zhǎng)因子-1(IGF-1)表達(dá)量。結(jié)果相比對(duì)照組,實(shí)驗(yàn)組低鐵干預(yù)4周后孕鼠孕末期Hb、HCT、MCV、MCH及MCHC等水平降低(P0.05),RDW水平升高(P0.05);實(shí)驗(yàn)組大鼠骨髓細(xì)胞內(nèi)鐵及外鐵減少;實(shí)驗(yàn)組新生仔鼠體重及仔鼠存活率低于對(duì)照組(P0.05);實(shí)驗(yàn)組垂體GH信使核糖核酸(mRNA)及肝臟IGF-1 mRNA的表達(dá)低于對(duì)照組(P0.05)。結(jié)論妊娠期鐵缺乏下調(diào)子代GH和IGF-1 mRNA表達(dá)造成子代低出生體重。
[Abstract]:Objective to investigate the effect and mechanism of iron deficiency during pregnancy on growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis in newborn rats. Methods Thirty-six 4-week-old female Wistar rats were randomly divided into experimental group and control group. Hematological parameters such as HCT, mean volume of red blood cell (MCV), mean hemoglobin content (MCHN), mean hemoglobin concentration (MCHCC) and distribution width of red blood cell (RDW) were measured. Bone marrow iron staining was performed at the end of pregnancy to detect the hematologic parameters of female mice and normal male mice. The survival rate and body weight were observed. The expression of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in liver of newborn rats were detected by real-time fluorescence quantitative polymerase chain reaction (QRT PCR). Results compared with the control group, the levels of HCT, MCV, MCH and MCHC in the late pregnant rats of the experimental group were decreased after the intervention of low iron for 4 weeks, and the levels of RDW in the bone marrow cells of the experimental group were increased, and the levels of iron and external iron in the bone marrow cells of the experimental group were decreased. The body weight and survival rate of newborn rats in the experimental group were lower than those in the control group (P 0.05), and the expression of GH mRNA mRNAs and IGF-1 mRNA in the liver in the experimental group were lower than those in the control group (P 0.05). Conclusion Iron deficiency during pregnancy can decrease the expression of GH and IGF-1 mRNA in offspring and result in low birth weight.
【作者單位】: 石河子大學(xué)醫(yī)學(xué)院;江蘇省漣水縣人民醫(yī)院;
【分類(lèi)號(hào)】:R714.254

【相似文獻(xiàn)】

相關(guān)期刊論文 前9條

1 馬雅靜;程江;;孕婦鐵缺乏與低出生體重研究進(jìn)展[J];農(nóng)墾醫(yī)學(xué);2009年04期

2 劉梅蘭;張建平;;圍產(chǎn)期鐵缺乏與補(bǔ)鐵[J];中華臨床醫(yī)師雜志(電子版);2010年05期

3 杜娟;孕婦鐵缺乏對(duì)母嬰健康的影響[J];華夏醫(yī)學(xué);2001年02期

4 廖清奎,李豐益;重視孕期鐵營(yíng)養(yǎng)、防治母嬰鐵缺乏[J];實(shí)用兒科臨床雜志;1997年03期

5 孫寬周;元紅;王亞秋;蔣玉紅;;孕婦體內(nèi)鐵缺乏對(duì)T淋巴細(xì)胞免疫活性的影響[J];中國(guó)優(yōu)生與遺傳雜志;2006年07期

6 付錦艷;楊安強(qiáng);劉e,

本文編號(hào):1953048


資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/fuchankeerkelunwen/1953048.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶d1eb2***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
欧美亚洲国产日韩一区二区| 蜜桃av人妻精品一区二区三区| 在线观看国产成人av天堂野外| 国产成人精品国内自产拍| 欧美日韩在线第一页日韩| 亚洲国产另类久久精品| 东京干男人都知道的天堂| 久久国产成人精品国产成人亚洲| 亚洲精品一区三区三区| 国产福利一区二区三区四区| 老司机精品福利视频在线播放| 国产日韩综合一区在线观看| 免费亚洲黄色在线观看| 国产一区二区三区草莓av| 尹人大香蕉一级片免费看| 伊人国产精选免费观看在线视频| 国产精品欧美在线观看| 色哟哟精品一区二区三区| 亚洲综合日韩精品欧美综合区| 亚洲欧洲精品一区二区三区| 国产又粗又猛又黄又爽视频免费| 久久综合亚洲精品蜜桃| 中文字幕亚洲精品乱码加勒比| 国产成人精品一区在线观看| 成年人视频日本大香蕉久久| 久久99一本色道亚洲精品| 丝袜av一区二区三区四区五区| 神马午夜福利免费视频| 富婆又大又白又丰满又紧又硬| 少妇高潮呻吟浪语91| 国产免费观看一区二区| 91福利免费一区二区三区| 色一欲一性一乱—区二区三区| 午夜精品在线观看视频午夜| 国产亚洲午夜高清国产拍精品| 欧美人禽色视频免费看| 91福利视频日本免费看看| 日本三区不卡高清更新二区| 一区中文字幕人妻少妇| 黄片美女在线免费观看| 日韩特级黄片免费观看|