本文選題：多囊卵巢綜合征 + 高雄激素血癥�。� 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的：比較高雄激素多囊卵巢綜合征患者(polycystic ovary syndrome,PCOS)組、非高雄激素PCOS患者組和正常對照組患者顆粒細(xì)胞中miR-21、miR-25、miR-132和miR-221的表達(dá)情況,探討雄激素相關(guān)microRNA在高雄激素PCOS發(fā)病機(jī)制中的可能作用。 方法：選擇2013年9月-2014年2月在中南大學(xué)湘雅醫(yī)院生殖中心實施體外受精／卵胞漿內(nèi)單精子顯微注射-胚胎移植(IVF／ICSI-ET)治療的女性不孕患者81例,其中高雄激素PCOS組(A組)23例,非高雄激素PCOS組(B組)27例,正常對照組(C組)31例。在取卵當(dāng)日,回收廢棄的卵泡液分離其中的顆粒細(xì)胞,進(jìn)行原代培養(yǎng)及鑒定,采用熒光定量PCR法檢測各組細(xì)胞中miR-21、miR-25、 miR-132和miR-221的表達(dá)差異情況,并與游離睪酮指數(shù)(FAI)進(jìn)行相關(guān)性分析。 結(jié)果：1.人黃素化顆粒細(xì)胞體外培養(yǎng)24h后貼壁,48h后呈典型顆粒細(xì)胞樣特征。2.A組miR-21和miR-25表達(dá)水平顯著低于其他兩組,差異有統(tǒng)計學(xué)意義(P0.05); miR-132和miR-221在三組患者中表達(dá)水平均無統(tǒng)計學(xué)差異(P0.05)。3.miR-21和miR-25的表達(dá)水平與FAI呈顯著負(fù)相關(guān)(r=-0.331,P=0.003；r=-0.285,P=0.01),差異有統(tǒng)計學(xué)意義。 結(jié)論：高雄激素PCOS患者顆粒細(xì)胞中的miR-21和miR-25表達(dá)降低可能與高雄激素PCOS患者發(fā)病機(jī)制有關(guān)。
[Abstract]:Objective: to compare the expression of miR-21 miR-25 miR-132 and miR-221 in granulosa cells of patients with hyperandrogen polycystic ovary syndrome (PCOS), non-hyperandrogen PCOS patients and normal controls. To explore the possible role of androgen related microRNA in the pathogenesis of hyperandrogen PCOS. Methods: from September 2013 to February 2014, 81 female infertile women who were treated with IVF / ICSI-ETT at the Reproductive Center of Xiangya Hospital, Central South University, were selected, including 23 cases of hyperandrogen PCOS group (group A). No hyperandrogen PCOS group (n = 27) and control group (n = 31). The granulosa cells were isolated from the waste follicular fluid on the day of egg retrieval. The expression of miR-21 miR-25, miR-132 and miR-221 were detected by fluorescence quantitative PCR assay. Correlation analysis was made with free testosterone index (FAI). The result is 1: 1. Human luteinized granulosa cells were cultured in vitro for 24 hours. The expression of miR-21 and miR-25 in group A was significantly lower than that in the other two groups. There was no significant difference in the expression levels of miR-132 and miR-221 between the three groups. There was a significant negative correlation between the expression of miR-132 and miR-25 and FAI. Conclusion: the decreased expression of miR-21 and miR-25 in granulosa cells of patients with hyperandrogen PCOS may be related to the pathogenesis of hyperandrogen PCOS.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R711.75

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