本文選題：多囊卵巢綜合征 + 胰島素抵抗　； 參考：《山東醫(yī)藥》2015年40期

【摘要】：目的探討艾塞那肽對脫氫表雄酮誘導的多囊卵巢綜合征(PCOS)大鼠胰島素抵抗及炎癥因子水平的影響。方法選擇SD大鼠30只,皮下注射脫氫表雄酮建立PCOS大鼠模型,隨機分為模型組、二甲雙胍組和艾塞那肽組,每組10只。另取SD大鼠10只作為正常組。成模后,二甲雙胍組、艾塞那肽組分別給予二甲雙胍0.265 g/(kg·d)灌胃、艾塞那肽10μg/(kg·d)皮下注射,正常組、模型組給予等量生理鹽水灌胃,均連續(xù)處理28天。觀察各組每周體質(zhì)量變化。實驗結(jié)束時行腹腔糖耐量試驗,檢測空腹血糖(FPG)、餐后2 h血糖(2 h PG)及胰島素(INS)水平;隔日下腔靜脈取血,ELISA法檢測血清睪酮(T)、卵泡刺激素(FSH)、黃體生成素(LH)、性激素結(jié)合球蛋白(SHBG)、IL-6、內(nèi)脂素(visfatin)和色素上皮細胞衍生因子(PEDF)水平。結(jié)果與正常組比較,模型組血清T、LH、LH/FSH、FPG、2 h PG、INS、HOMA-IR、IL-6、PEDF水平均顯著升高(P均0.05)。與模型組比較,二甲雙胍組、艾塞那肽組血清T、LH、LH/FSH、FPG、2 h PG、INS、HOMA-IR、IL-6、PEDF、visfatin水平均顯著降低(P均0.05),FSH、SHBG水平均顯著升高(P均0.05);二甲雙胍組、艾塞那肽組各指標比較差異無統(tǒng)計學意義(P均0.05)。與模型組比較,艾塞那肽組、二甲雙胍組體質(zhì)量均降低(P均0.05),且艾塞那肽組體質(zhì)量降低的時間更早。結(jié)論艾塞那肽在改善PCOS大鼠胰島素抵抗及降低炎癥因子水平方面與二甲雙胍相當,但其降低體質(zhì)量的起效時間較二甲雙胍更早。
[Abstract]:Objective to investigate the effects of isenapeptide on insulin resistance and inflammatory factors in rats with dehydroepiandrosterone induced polycystic ovary syndrome (PCOS). Methods Thirty SD rats were randomly divided into three groups: model group, metformin group and isenapeptide group, with 10 rats in each group. Another 10 SD rats were taken as normal group. After the model, metformin group and Isenapeptide group were given metformin 0.265 g/(kg d, Isenapeptide 10 渭 g/(kg d) subcutaneously. The normal group and model group were treated with the same amount of normal saline for 28 days. The changes of weekly body mass in each group were observed. At the end of the experiment, intraperitoneal glucose tolerance test was performed to detect the levels of fasting blood glucose (FPG), 2 h postprandial glucose (PGN) and insulin (ins). The levels of serum testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), sex hormone binding globulin (SHBG) IL-6 (visfatin) and pigment epithelium-derived factor (PEDFs) were detected by Elisa. Results compared with the normal group, the serum levels of serum TLH / FSHH / FSHFPGN in the model group were significantly higher than those in the control group (P < 0.05). Compared with the model group, the serum levels of TLH / LH / FSHFPGN in metformin group and Eisenapeptide group were significantly lower than those in the metformin group and the isenapeptide group (P < 0.05), but there was no significant difference between the two groups (P = 0.05). Compared with the model group, the body mass of both the Aisenapeptide group and metformin group were all decreased by 0.05%, and the time of the decrease of the body mass in the Isenapeptide group was earlier than that in the control group. Conclusion Isenapeptide is similar to metformin in improving insulin resistance and reducing the level of inflammatory cytokines in PCOS rats, but its effect time of reducing body mass is earlier than that of metformin.
【作者單位】： 南京大學醫(yī)學院附屬南京鼓樓醫(yī)院集團宿遷市人民醫(yī)院;南京大學醫(yī)學院附屬鼓樓醫(yī)院;
【基金】：南京市衛(wèi)生青年人才培養(yǎng)工程項目(ZKX11017) 南京市醫(yī)學科技發(fā)展項目(YKK13075)
【分類號】：R711.75

【參考文獻】

相關(guān)期刊論文 前5條

1 張曉薇,鄺健全,曾愛群,劉慧姝;脫氫表雄酮誘導多囊卵巢綜合征動物模型的研究[J];廣州醫(yī)學院學報;2000年03期

2 付玉玲;王燕;周春;;青春期多囊卵巢綜合征動物模型的實驗研究[J];武漢大學學報(醫(yī)學版);2007年06期

3 張惠英;于風華;霍琰;田麗;牛鳳霞;;二甲雙胍對PCOS患者血清慢性炎癥因子及血脂的影響[J];河北醫(yī)學;2009年02期

4 洪嶺;童曉文;;多囊卵巢綜合征與炎癥因子[J];中華婦產(chǎn)科雜志;2007年10期

5 楊學敏;古健;沈慧敏;李田;鄒先翔;蘇淑軍;張?zhí)硭?;血清及卵泡液內(nèi)脂素水平與多囊卵巢綜合征的關(guān)系[J];中山大學學報(醫(yī)學科學版);2013年02期

【共引文獻】

相關(guān)期刊論文 前10條

1 曾蕾;趙紅艷;羅頌平;;改良PCOS-IR大鼠模型的設(shè)計與實驗[J];中華中醫(yī)藥雜志;2010年12期

2 劉宇濤;夏薇;韋笑天;馬維軍;周聰;王偉;石放雄;;脫氫表雄酮誘導大鼠多囊卵巢綜合征的研究[J];動物醫(yī)學進展;2008年09期

3 李燕;張慶文;王紅彬;;脫氫表雄酮誘導SD大鼠多囊卵巢綜合征模型的實驗研究[J];中國醫(yī)藥科學;2013年07期

4 張?zhí)硭?鄒先翔;蘇淑軍;李田;萬t，

本文編號：1920311