本文選題：宮頸癌前病變 + 人乳頭瘤病毒��； 參考：《大連醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：本研究旨在通過檢測HPV感染和不同程度的宮頸病變（CIN2-3）患者陰道沖洗液的細(xì)胞因子（IL-2）和一氧化氮（NO）的水平，探討IL-2、NO和CIN之間是否相關(guān)，IL-2和NO的趨勢，陰道局部免疫與CIN的關(guān)系，為預(yù)防宮頸癌的發(fā)生提供了可能。 方法：收集2012-2013年大連市婦幼保健院婦科門診收治的18-65歲并且陰道鏡下活檢確診為HPV感染的患者30例，陰道鏡下活檢確診為HPV感染同時為CIN2-3的患者30例，同期正常體檢的健康婦女30例作對照組。剔除標(biāo)準(zhǔn):妊娠及哺乳期，服用性激素婦女；陰道病變，陰道流血，l周內(nèi)有陰道用藥史，3天內(nèi)性生活史；有長期使用皮質(zhì)類固醇激素及免疫抑制劑用藥史者；2周內(nèi)全身使用抗生索的患者。標(biāo)本的采集均經(jīng)研究對象的知情同意。本實(shí)驗(yàn)通過酶聯(lián)免疫吸附試驗(yàn)法即ELISA法來檢測HPV、CIN2-3及正常健康婦女陰道局部細(xì)胞因子IL-2、NO的水平。全部數(shù)據(jù)應(yīng)用SPSS13.0統(tǒng)計軟件進(jìn)行數(shù)據(jù)處理分析。 結(jié)果： 1.兩組一般情況的比較HPV組、HPV+CIN2-3組及對照組，三組婦女在年齡、懷孕次數(shù)、性伴侶個數(shù)、第一次性生活年齡、吸煙、口服避孕藥以及每年體檢（TCT）、家族遺傳相比較，(p0.05)差異均無統(tǒng)計學(xué)意義。 2. HPV組與對照組比較：IL-2水平明顯降低（P0.01）;NO水平明顯上升（P0.01） 3.CIN2-3組與對照組比較：IL-2水平明顯降低（P0.01）；NO水平明顯上升（P0.01）。 4.CIN2-3組與HPV組比較：IL-2水平下降（P0.05），NO水平上升（P0.05）。 結(jié)論： CIN雖屬于連續(xù)發(fā)展病變，但也存在極大逆轉(zhuǎn)可能，且CIN發(fā)展為宮頸癌常需要8-10年。因此，深刻認(rèn)識免疫因素在CIN轉(zhuǎn)歸中作用及微量元素與機(jī)體免疫狀態(tài)關(guān)系，可為CIN和宮頸癌的輔助治療、預(yù)防開拓新視野，提供新的研究方向和治療思路。本實(shí)驗(yàn)證實(shí)了：1.IL-2和NO與CIN相關(guān)。2.隨著宮頸病變的發(fā)展IL-2呈下降趨勢，NO呈上升趨勢。3.陰道局部免疫功能下降是CIN發(fā)生，，發(fā)展及演變的重要因素之一。
[Abstract]:Objective: to investigate the relationship between IL 2 no and CIN by detecting the levels of cytokine 2 (IL 2) and nitric oxide (no) in vaginal lavage fluid of patients with HPV infection and cervix cervicitis (CIN2-3), and to explore whether there is a correlation between IL 2 no and CIN. The relationship between vaginal local immunity and CIN provides the possibility for the prevention of cervical cancer. Methods: thirty patients aged 18-65 years with HPV infection diagnosed by colposcopy biopsy and 30 patients with HPV infection and CIN2-3 diagnosed by colposcopy biopsy were collected from the gynecological outpatient department of Dalian Maternal and Child Health Hospital from 2012 to 2013. At the same time, 30 healthy women who had normal physical examination served as control group. Exclusion criteria: pregnancy and lactation, taking sex hormone for women, vaginopathy, vaginal bleeding within 1 week history of vaginal medication and sexual history within 3 days; Patients with a long history of corticosteroid use and immunosuppressant use throughout the body within 2 weeks. All specimens were collected with the informed consent of the subjects. In this study, the levels of CIN2-3 and IL-2no in vaginal area of healthy women were detected by enzyme linked immunosorbent assay (ELISA). All data are processed and analyzed by SPSS13.0 statistical software. Results: 1. Comparison of the general conditions between the two groups: HPV CIN2-3 group and control group, three groups of women in age, number of pregnancies, number of sexual partners, first life age, smoking, There was no significant difference in family heredity between oral contraceptive pills and annual physical check-up (P 0.05). 2. Compared with the control group, the level of IL-2 in the HPV group was significantly lower than that in the control group. Compared with the control group, the level of IL-2 in the 3.CIN2-3 group was significantly lower than that in the control group. Compared with HPV group, the level of IL-2 in 4.CIN2-3 group was lower than that in HPV group, and the level of no in 4.CIN2-3 group was higher than that in HPV group. Conclusion: Although CIN is a progressive lesion, it may be reversed greatly, and it usually takes 8 to 10 years for CIN to develop into cervical cancer. Therefore, a profound understanding of the role of immune factors in the outcome of CIN and the relationship between trace elements and immune status of the body can provide a new research direction and treatment ideas for the adjuvant treatment of CIN and cervical cancer, the development of new horizons for prevention and treatment. This experiment confirmed that: 1. IL-2 and no are related to CIN. With the development of cervical lesions, IL-2 showed a downward trend. The decrease of vaginal local immune function is one of the important factors in the occurrence, development and evolution of CIN.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.33

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 魏蘭蘭;劉爽;谷鴻喜;張鳳民;;一氧化氮、人乳頭瘤病毒和宮頸癌之間相互關(guān)系的研究進(jìn)展[J];微生物與感染;2011年01期

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