發(fā)布時間：2018-05-16 15:48

  本文選題：NDRG + 增殖��； 參考：《實用醫(yī)學雜志》2016年22期

【摘要】：目的:研究N-MYC下游調控基因4在宮頸癌細胞中的表達及其對宮頸癌細胞增殖、凋亡及周期的影響。方法:通過熒光實時定量聚合酶鏈反應和免疫印跡檢測HeLa細胞中NDRG4的表達,構建NDRG4穩(wěn)轉細胞系HeLa-NDRG4。CCK-8法檢測NDRG4對Hela細胞增殖的影響,流式細胞術檢測NDRG4對Hela細胞凋亡及周期的影響。結果:宮頸癌細胞中NDRG4 m RNA和蛋白表達水平均較人正常宮頸內皮細胞顯著降低,HeLa-NDRG4細胞的增殖能力顯著低于HeLa-MOCK細胞,而凋亡能力顯著高于HeLa-MOCK細胞。與HeLa-MOCK細胞相比,HeLa-NDRG4細胞周期被阻滯于G1期。HeLa-NDRG4細胞中p-Akt的蛋白水平較HeLa-MOCK細胞顯著降低,而PTEN的蛋白水平較HeLa-MOCK細胞顯著升高。結論 :NDRG4在宮頸癌中作為抑癌基因發(fā)揮作用。
[Abstract]:Aim: to investigate the expression of N-MYC downstream regulatory gene 4 in cervical cancer cells and its effects on cell proliferation, apoptosis and cell cycle. Methods: the expression of NDRG4 in HeLa cells was detected by fluorescence real-time quantitative polymerase chain reaction and Western blotting. The effect of NDRG4 on the proliferation of Hela cells was detected by HeLa-NDRG4.CCK-8 method, and the effect of NDRG4 on apoptosis and cell cycle of Hela cells was detected by flow cytometry. Results: the expression levels of NDRG4 m RNA and protein in cervical cancer cells were significantly lower than those in normal cervical endothelial cells. The proliferative ability of HeLa-NDRG4 cells was significantly lower than that of HeLa-MOCK cells, and the apoptotic ability was significantly higher than that of HeLa-MOCK cells. Compared with HeLa-MOCK cells, the cell cycle of HeLa-NDRG4 cells was blocked in G1 phase. The protein level of p-Akt in He-La-NDRG4 cells was significantly lower than that of HeLa-MOCK cells, while the protein level of PTEN was significantly higher than that of HeLa-MOCK cells. Conclusion the expression of the tumor suppressor gene in cervix carcinoma may be affected by the expression of 1% NDRG 4.
【作者單位】： 西南醫(yī)科大學臨床醫(yī)學院;綿陽市中心醫(yī)院婦產科;
【分類號】：R737.33

【相似文獻】

相關期刊論文 前10條

1 盧美松;鄧鎖;王澤華;;體外模擬二氧化碳人工氣腹對宮頸癌細胞生長的影響[J];中國實用婦科與產科雜志;2007年01期

2 周晨慧;;干擾素-γ對宮頸癌細胞免疫分子表達的影響[J];現代醫(yī)藥衛(wèi)生;2007年01期

3 潘惠艷;趙群;詹陽;趙麗紅;張衛(wèi)華;吳玉梅;;電壓門控鈉離子通道表達對宮頸癌細胞增殖侵襲轉移作用的研究[J];中國腫瘤臨床;2012年04期

4 馮理達;錢菊卿;陳淑琴;張s，

本文編號：1897471