本文選題：先兆子癇 + 瞬時(shí)受體電位通道�。� 參考：《青島大學(xué)》2014年碩士論文

【摘要】：目的探討瞬時(shí)感受器陽(yáng)離子通道6(transient receptor potential channel6, TRPC6)與子癇前期發(fā)病的關(guān)系。 方法選取2012年8月-2013年1月在青島大學(xué)附屬醫(yī)院產(chǎn)科住院的輕度子癇前期孕婦20例(輕度子癇前期組),重度子癇前期孕婦(重度子癇前期組)23例以及因社會(huì)因素和頭盆不稱因素剖宮產(chǎn)患者(對(duì)照組)25例。采用酶聯(lián)免疫吸附試驗(yàn)(ELISA)檢測(cè)三組孕婦血清中TRPC6的水平,并分析各組孕婦血清TRPC6與血壓、生化指標(biāo)和24h尿蛋白總量的相關(guān)性。采用熒光定量PCR檢測(cè)上述各組受試者各組織(胎盤(pán)及子宮平滑肌)中TRPC6的mRNA含量。采用WESTEN-BLOT方法檢測(cè)胎盤(pán)及子宮平滑肌組織中TRPC6的蛋白表達(dá)。并分析血清TRPC6與各組織TRPC6mRNA及蛋白的相關(guān)性。 結(jié)果(1)血清TRPC6水平：輕度子癇前期組及重度子癇前期組均高于對(duì)照組,差異有顯著性(F=5.98,q=3.43,q=4.68,P0.05)；輕度子癇前期組和重度子癇前期組水平差異無(wú)顯著性(q=1.05,P0.05)。在輕度子癇前期組與重度子癇前期組中,血清TRPC6水平與血壓(收縮壓及舒張壓均)呈正相關(guān)(r=0.712、0.774,P0.05)；與24h尿蛋白總量均呈正相關(guān)(r=0.841、0.897,P0.05)；與甘油三酯(TG)、總膽固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)均無(wú)相關(guān)性(P0.05)。(2)胎盤(pán)組織中TRPC6mRNA表達(dá)水平在輕度子癇前期組及重度子癇前期組孕婦中分別高于正常組的2.1倍和2.7倍,分別比較,差異均有統(tǒng)計(jì)學(xué)意義(F=9.99,q=4.88,q=5.90,P0.05)；子宮平滑肌中TRPC6mRNA表達(dá)水平在輕度組及重度組孕婦中分別高于正常組的6倍和10倍,分別比較,差異均有統(tǒng)計(jì)學(xué)意義(F=41.38,q=9.49,q=12.38,P0.05)；胎盤(pán)和子宮平滑肌的TRPC6mRNA表達(dá)水平,在輕度組和重度組中的差異無(wú)顯著性(q=0.79、2.34,P0.05)。(3)胎盤(pán)組織中TRPC6蛋白在輕度子癇前期組及重度子癇前期組孕婦的表達(dá)水平分別為0.86±0.33,1.09±0.302,明顯高于對(duì)照組的0.54±0.254,差異有顯著性(F=10.08,q輕,對(duì)=5.08,q重,對(duì)=9.17,q輕,重=3.59,P0.05)；子宮平滑肌中TRPC6蛋白的表達(dá)水平分別為1.00±0.175,1.18±0.134,明顯高于對(duì)照組的0.65±0.245,差異有顯著性(F=20.10,q輕,對(duì)=8.75,q重,對(duì)=13.59,q輕,重=4.39,P0.05)(4)血清TRPC6水平與胎盤(pán)的TRPC6mRNA及蛋白均呈正相關(guān)(r=0.31,r=1.37,P0.05),血清TRPC6水平與子宮平滑肌的TRPC6mRNA無(wú)相關(guān)性(r=0.11,P0.05),與子宮平滑肌的TRPC6蛋白呈正相關(guān)(r=1.36,P0.05)。 結(jié)論子癇前期患者胎盤(pán)組織及子宮平滑肌中TRPC6mRNA表達(dá)水平上調(diào)及TRPC6蛋白表達(dá)增加,可能引起血清中TRPC6水平升高。血管內(nèi)皮細(xì)胞受損,導(dǎo)致的血管平滑肌細(xì)胞上TRPC6通道激活可能與子癇前期發(fā)生發(fā)展有關(guān)。
[Abstract]:Objective to investigate the relationship between transient receptor cationic channel 6(transient receptor potential channel 6 (TRPC6) and preeclampsia. Methods from August 2012 to January 2013, 20 pregnant women with mild preeclampsia (mild preeclampsia), severe preeclampsia (23 severe preeclampsia) and social factors were selected. And cephalopelvic disproportion factor cesarean section (control group, 25 cases). The levels of TRPC6 in serum of three groups of pregnant women were detected by Elisa, and the correlation between serum TRPC6 and blood pressure, biochemical index and total amount of 24 hours urine protein was analyzed. The content of TRPC6 mRNA in placenta and uterine smooth muscle was measured by fluorescence quantitative PCR. The expression of TRPC6 protein in placenta and uterine smooth muscle tissue was detected by WESTEN-BLOT method. The correlation between serum TRPC6 and TRPC6mRNA and protein was analyzed. Results 1) the level of serum TRPC6 in mild preeclampsia group and severe preeclampsia group was higher than that in control group, the difference was significant (P 0.05), and there was no significant difference between mild preeclampsia group and severe preeclampsia group. In the mild preeclampsia group and the severe preeclampsia group, Serum TRPC6 levels were positively correlated with blood pressure (systolic blood pressure and diastolic blood pressure), 0.712 ~ 0.774U / P 0.05, r ~ (0.841) / 0.897p _ (0.05), respectively, and had no correlation with triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDLs), and placenta group (P _ (0.05) n ~ (-1). The expression of TRPC6mRNA in pregnant women with mild preeclampsia and severe preeclampsia was 2. 1 and 2. 7 times higher than that of normal women, respectively. The difference was statistically significant (P 0.05), and the expression of TRPC6mRNA in the uterine smooth muscle was 6 times and 10 times higher in the mild and severe pregnant women than in the normal group, respectively. The difference was statistically significant (P < 0.05). The expression of TRPC6mRNA in placenta and uterine smooth muscle was significantly higher than that in normal placenta and uterus. There was no significant difference between mild group and severe group. The expression of TRPC6 protein in placenta tissue was 0.86 鹵0.33 1.09 鹵0.302 in mild preeclampsia group and severe preeclampsia group respectively, which was significantly higher than that in control group (0.54 鹵0.254). There was significant difference in the expression of TRPC6 protein between mild preeclampsia group and severe preeclampsia group (P < 0.05), and the difference was significant (P < 0.05), and the expression of TRPC6 protein in pregnant women with mild preeclampsia and severe preeclampsia was 0.86 鹵0.31.09 鹵0.302, respectively, which was significantly higher than that of control group (0.54 鹵0.254). The expression levels of TRPC6 protein in uterine smooth muscle were 1.00 鹵0.175 ~ 1.18 鹵0.134, respectively, which were significantly higher than those in the control group (0.65 鹵0.245). The difference was significant (P < 0.05). The expression of TRPC6 protein in uterine smooth muscle was significantly higher than that in the control group (0.65 鹵0.245). There was a positive correlation between serum TRPC6 level and placental TRPC6mRNA and protein. There was no correlation between serum TRPC6 level and TRPC6mRNA of uterine smooth muscle, and there was a positive correlation between serum TRPC6 level and TRPC6 protein of uterine smooth muscle. Conclusion the expression of TRPC6mRNA and the expression of TRPC6 protein in placenta and uterine smooth muscle of preeclampsia patients are up-regulated, which may lead to the increase of TRPC6 level in serum. The activation of TRPC6 channels in vascular smooth muscle cells may be related to the development of preeclampsia.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R714.244

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