本文選題：HLA-DQB + 宮頸癌�。� 參考：《中國(guó)免疫學(xué)雜志》2017年04期

【摘要】：目的:探討HLA-DQB1等位基因多態(tài)性對(duì)廣西壯族女性HPV16感染和宮頸癌發(fā)生的影響,為尋找廣西壯族女性宮頸癌的遺傳易感基因或保護(hù)基因提供線(xiàn)索。方法:選取廣西地區(qū)25~45歲宮頸癌確診的壯族患者、無(wú)癌健康壯族女性各171例作為研究對(duì)象(按年齡±3歲配對(duì)),采集研究對(duì)象樣本并提取HPV核酸和人基因組DNA,分別應(yīng)用PCR-SSP和分子導(dǎo)流雜交技術(shù)進(jìn)行HLA-DQB1基因型檢測(cè)和HPV基因分型檢測(cè),最后進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:(1)171例宮頸癌患者中HPV總感染率為91.22%,其中高危型病毒占90.76%,HPV16型為主要致病亞型(43.58%);(2)廣西壯族女性宮頸癌患者組的HLA-DQB1*04等位基因攜帶率高于無(wú)癌對(duì)照組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);等位基因HLA-DQB1*06/09在宮頸癌患者組中的攜帶率明顯低于無(wú)癌組,差異具有統(tǒng)計(jì)學(xué)意義(P0.05);而兩組間的HLA-DQB1*02/05/07/08等位基因攜帶率無(wú)顯著性差異(P0.05);(3)HLA-DQB1*04基因在HPV16陽(yáng)性宮頸癌患者中出現(xiàn)的頻率明顯高于HPV16陰性患者,差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:HLA-DQB1*04可能是廣西壯族女性宮頸癌發(fā)生的易感基因,HLA-DQB1*06/09可能是廣西壯族女性宮頸癌的保護(hù)基因。而HLA-DQB1*02/05/07/08等位基因可能與廣西壯族女性宮頸癌遺傳易感性無(wú)關(guān)。攜帶HLA-DQB1*04等位基因的廣西壯族婦女可能更容易感染HPV16型病毒,從而增加了其患宮頸癌的危險(xiǎn)性。
[Abstract]:Objective: to investigate the effect of HLA-DQB1 allele polymorphism on HPV16 infection and cervical cancer in Zhuang women in Guangxi. Methods: selected 25 ~ 45 years old patients with cervical cancer in Guangxi, A total of 171 healthy Zhuang women (age 鹵3 years old) were selected as the study subjects. HPV nucleic acid and human genomic DNA were collected, and HLA-DQB1 genotypes and HPV genotypes were detected by PCR-SSP and molecular diversion hybridization techniques, respectively. Finally, statistical analysis was carried out. Results the total infection rate of HPV in 171 patients with cervical cancer was 91.22. The rate of HLA-DQB1*04 alleles in Guangxi Zhuang women with cervical cancer was higher than that in non-cancer control group (90.76% HPV16 was the main pathogenic subtype of HPV16). The frequency of allelic HLA-DQB1*06/09 was significantly lower in patients with cervical cancer than that in patients without cervical cancer, the difference was statistically significant (P 0.05), and the frequency of allelic HLA-DQB1*06/09 was significantly lower in patients with cervical cancer. There was no significant difference in the frequency of HLA-DQB1*02/05/07/08 alleles between the two groups. The frequency of HLA-DQB1n04 gene in patients with HPV16 positive cervical cancer was significantly higher than that in patients with HPV16 negative, and the difference was statistically significant (P 0.05). Conclusion: HLA-DQB1O4 may be the susceptible gene of cervical cancer in Zhuang women in Guangxi. HLA-DQB1 / 06 / 09 may be a protective gene for cervical cancer in Zhuang women in Guangxi. The HLA-DQB1*02/05/07/08 allele may not be associated with the genetic susceptibility of cervical cancer in Zhuang women in Guangxi. Guangxi Zhuang women with HLA-DQB1*04 allele were more likely to be infected with HPV16, which increased their risk of cervical cancer.
【作者單位】： 廣西衛(wèi)生職業(yè)技術(shù)學(xué)院;
【基金】：廣西壯族自治區(qū)教育廳2016年度廣西高校中青年教師基礎(chǔ)能力提升項(xiàng)目(No.KY2016YB740) 廣西壯族自治區(qū)教育廳項(xiàng)目(No.GXGZJG2015B240)
【分類(lèi)號(hào)】：R737.33

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙富璽;孫瑞芳;王晉芬;陳向偉;;應(yīng)用組織芯片技術(shù)研究人宮頸鱗狀細(xì)胞癌及宮頸上皮內(nèi)瘤樣變組織中HPV16感染與端粒酶hTERT表達(dá)的關(guān)系[J];大同醫(yī)學(xué)專(zhuān)科學(xué)校學(xué)報(bào);2006年01期

2 周曉燕,朱偉萍,金愛(ài)萍,施達(dá)仁;乳腺浸潤(rùn)性導(dǎo)管癌中HPV18、HPV16感染的研究[J];臨床與實(shí)驗(yàn)病理學(xué)雜志;2002年04期

3 史永華;拉萊·蘇祖克;陳曉;;子宮頸鱗狀細(xì)胞癌中P53、MDM2的表達(dá)與HPV16感染的關(guān)系[J];醫(yī)學(xué)研究與教育;2009年02期

4 陳欽忠,亓天偉,趙溫利;HPV16感染與膀胱癌的相關(guān)性研究[J];山東醫(yī)科大學(xué)學(xué)報(bào);1997年03期

5 孫圣榮,張子蕾,姚峰;胃癌及癌旁組織中HPV16感染與p21基因失活的相關(guān)研究[J];中華實(shí)驗(yàn)外科雜志;2001年04期

6 蔡吉亮;孫金中;姚峰;孫圣榮;;HPV16感染與P21基因失活在胃癌發(fā)生及預(yù)后中的意義(英文)[J];The Chinese-German Journal of Clinical Oncology;2006年02期

7 馬振祥;顧曉梅;姜聲揚(yáng);張璇;顧桂芬;莊勛;;國(guó)內(nèi)HPV16感染與宮頸癌發(fā)病風(fēng)險(xiǎn)的Meta分析[J];現(xiàn)代預(yù)防醫(yī)學(xué);2008年24期

8 嚴(yán)志凌;周紅林;張紅平;劉熙;張芍;尹春梅;楊宏英;;宿主p53基因多態(tài)性與HPV16感染、宮頸癌發(fā)生發(fā)展的相關(guān)性[J];昆明醫(yī)科大學(xué)學(xué)報(bào);2014年05期

9 王燕;古力娜爾·庫(kù)爾班;阿仙姑·哈斯木;塔拉甫·托坎;;子宮頸癌中c-myc和hMLH1表達(dá)與HPV16感染的關(guān)系[J];臨床與實(shí)驗(yàn)病理學(xué)雜志;2012年02期

10 阿仙姑·哈斯木;李巧稚;馬俊旗;古麗扎熱葉·艾庫(kù)爾;阿布力孜·阿布杜拉;;維吾爾族患者宮頸病變中內(nèi)質(zhì)網(wǎng)分子伴侶和HLA-Ⅰ類(lèi)分子表達(dá)與HPV16感染的關(guān)系研究[J];實(shí)用婦產(chǎn)科雜志;2011年02期

相關(guān)會(huì)議論文 前2條

1 周曉燕;朱偉萍;金愛(ài)萍;施達(dá)仁;;乳腺浸潤(rùn)性導(dǎo)管癌中HPV18、HPV16感染的研究[A];2000全國(guó)腫瘤學(xué)術(shù)大會(huì)論文集[C];2000年

2 何謙;;乳腺癌中HPV16感染與c-erbB-2、bcl-2表達(dá)的關(guān)系分析[A];中華醫(yī)學(xué)會(huì)第七次全國(guó)檢驗(yàn)醫(yī)學(xué)學(xué)術(shù)會(huì)議資料匯編[C];2008年

相關(guān)碩士學(xué)位論文 前2條

1 尹春梅;宿主p53基因多態(tài)性與HPV16感染宮頸癌相關(guān)性的研究[D];昆明醫(yī)科大學(xué);2015年

2 張金莉;新疆喀什夏普陶勒鄉(xiāng)維吾爾族婦女宮頸病變及HPV16感染的調(diào)查研究[D];石河子大學(xué);2008年

，

本文編號(hào)：1895988