本文選題：PTEN + 子癇前期　； 參考：《現(xiàn)代婦產(chǎn)科進(jìn)展》2017年10期

【摘要】：目的:檢測(cè)子癇前期(PE)患者胎盤組織中PTEN表達(dá),探討PTEN對(duì)滋養(yǎng)細(xì)胞遷移功能的調(diào)控及其可能機(jī)制。方法:收集重度PE患者和正常孕婦的胎盤組織各20例,實(shí)時(shí)定量PCR和Western blot法檢測(cè)胎盤組織中PTEN表達(dá);將PTEN過表達(dá)質(zhì)粒(pcDNA3.1-HA-PTEN)或特異性siRNA(siPTEN)瞬時(shí)轉(zhuǎn)染至HTR-8/SVneo細(xì)胞,同時(shí)轉(zhuǎn)染pcDNA3.1或siCTL作為對(duì)照。采用劃痕實(shí)驗(yàn)評(píng)估細(xì)胞遷移能力,Western blot法檢測(cè)p-Akt(Thr308)、p-Akt(Ser473)、Akt、MMP-2和MMP-9表達(dá)。結(jié)果:重度PE患者胎盤組織中PTEN mRNA和蛋白表達(dá)明顯高于正常孕婦,差異均有統(tǒng)計(jì)學(xué)意義(P0.01)。與轉(zhuǎn)染pcDNA3.1組比較,pcDNA3.1-HA-PTEN瞬時(shí)轉(zhuǎn)染HTR-8/SVneo細(xì)胞48h后,細(xì)胞遷移率明顯降低[(26.42±6.68)%vs(52.92±6.71)%,P0.01],p-Akt(Thr308)和p-Akt(Ser473)表達(dá)顯著下調(diào),Akt表達(dá)無明顯改變,MMP-2和MMP-9蛋白表達(dá)下降;與轉(zhuǎn)染siCTL組比較,siPTEN瞬時(shí)轉(zhuǎn)染HTR-8/SVneo細(xì)胞48h后,細(xì)胞遷移率則明顯升高[(50.39±6.84)%vs(30.04±5.40)%,P0.01],p-Akt(Thr308)和p-Akt(Ser473)的表達(dá)明顯上調(diào),Akt表達(dá)無明顯改變,MMP-2和MMP-9蛋白表達(dá)升高。結(jié)論:重度PE患者胎盤組織中PTEN表達(dá)明顯升高,PTEN可通過下調(diào)Akt活性降低MMP-2和MMP-9表達(dá),抑制滋養(yǎng)細(xì)胞的遷移。提示PTEN在PE的發(fā)病中發(fā)揮了一定的作用。
[Abstract]:Aim: to investigate the expression of PTEN in placenta of patients with preeclampsia and to explore the regulation of trophoblast migration by PTEN and its possible mechanism. Methods: the placental tissues of 20 patients with severe PE and 20 normal pregnant women were collected to detect the expression of PTEN in placenta tissue by real-time quantitative PCR and Western blot, and the PTEN overexpression plasmid pcDNA3.1-HA-PTEN) or specific siRNAsiPTEN) was transfected into HTR-8/SVneo cells. At the same time, pcDNA3.1 or siCTL were transfected as control. The cell migration ability was evaluated by scratch assay. The expression of p-Akttttttsil, Thr308, p-AktSer 473 and AktTU MMP-2 and MMP-9 were detected by Western blot assay. Results: the expression of PTEN mRNA and protein in placenta of severe PE patients was significantly higher than that of normal pregnant women (P 0.01). Compared with the pcDNA3.1 transfection group, the cell migration rate of HTR-8/SVneo cells transfected with pcDNA3.1-HA-PTEN for 48h was significantly decreased [26.42 鹵6.68)%vs(52.92 鹵6.71p0.01] and p-Akttsi-Thr308), and the expression of MMP-2 and MMP-9 protein was not significantly decreased after transfection of siCTL, and the expression of MMP-2 and MMP-9 protein was decreased after transient transfection of HTR-8/SVneo cells 48 h after transfection of siCTL, and the expression of MMP 2 and MMP-9 protein decreased significantly after transfection of ppcDNA3.1-HA-PTEN, and the expression of MMP 2 and MMP-9 protein decreased significantly after transfection of ppcDNA3.1-HA-PTEN. The cell migration rate was significantly increased [50.39 鹵5.40 6.84)%vs(30.04 鹵5.40 + P0.01] and p-AktTU Thr308 and p-AktTU Ser473) were significantly up-regulated. No significant changes in the expression of MMP-2 and MMP-9 protein were observed. Conclusion: the expression of PTEN in placenta of severe PE patients was significantly increased. PTEN could decrease the expression of MMP-2 and MMP-9 and inhibit the migration of trophoblast by down-regulating the activity of Akt. The results suggest that PTEN may play a role in the pathogenesis of PE.
【作者單位】： 南京醫(yī)科大學(xué)附屬常州婦幼保健院婦產(chǎn)科;南京醫(yī)科大學(xué)附屬常州第二人民醫(yī)院乳腺外科;南京大學(xué)醫(yī)學(xué)院附屬鼓樓醫(yī)院婦產(chǎn)科;
【基金】：南京醫(yī)科大學(xué)科技發(fā)展基金重點(diǎn)項(xiàng)目(No:2016NJMUZD095)
【分類號(hào)】：R714.244

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