本文選題：維生素A + 肝臟�。� 參考：《重慶醫(yī)學》2017年16期

【摘要】：目的通過孕期維生素A(VA)缺乏大鼠模型,探討孕期VA缺乏對子代肝臟組織中脂質合成的影響。方法建立大鼠孕期VA缺乏模型,分VA正常(VAN)、VA缺乏(VAD)、VA缺乏后補充(VAS)3組,檢測子代血脂水平;肝臟中乙酰輔酶A羧化酶1(ACC1)、脂肪酸合成酶(FAS)、固醇調節(jié)元件結合蛋白(SREBP1)等脂質合成分子的mRNA表達變化;觀察子代肝臟組織切片HE染色后脂滴沉積情況。結果 VAD組的HDL-C水平顯著低于VAN組和VAS組(P0.05),而VAN組和VAS組之間差異無統(tǒng)計學意義(P0.05)。TG水平在3組間差異有統(tǒng)計學意義(P0.05)。VAD組ACC1、FAS、SREBP1mRNA表達水平顯著增加。VAD組肝臟出現較多的脂滴沉積,細胞質中有部分脂滴空泡;而VAS組和VAN組肝細胞排列規(guī)則,未見明顯脂滴沉積。結論孕期VA缺乏可誘發(fā)大鼠子代肝臟脂質合成通路的異常活化,造成質脂代謝紊亂。
[Abstract]:Objective to investigate the effect of vitamin A (VA) deficiency during pregnancy on lipid synthesis in liver tissue of offspring. Methods A model of VA deficiency during pregnancy was established in rats. The rats were divided into three groups: the normal VA group was supplemented with VASA deficiency group, and the blood lipid level of the offspring was detected. The changes of mRNA expression of lipids such as acetyl coA carboxylase 1 (ACC1), fatty acid synthase (FASA) and steroid regulatory element binding protein (SREBP1) in liver were observed. Results the level of HDL-C in VAD group was significantly lower than that in VAN group and VAS group, but there was no significant difference between VAN group and VAS group. There were some lipid droplets in the cytoplasm, but there was no obvious lipid droplet deposition in the liver cells of VAS group and VAN group. Conclusion during pregnancy, VA deficiency can induce abnormal activation of lipid synthesis pathway in rat offspring and lead to disorder of lipid metabolism.
【作者單位】： 重慶醫(yī)科大學附屬兒童醫(yī)院營養(yǎng)研究中心;兒童發(fā)育疾病研究教育部重點實驗室;重慶醫(yī)科大學附屬兒童醫(yī)院門診部;重慶醫(yī)科大學附屬第一醫(yī)院脂糖代謝實驗室;
【基金】：國家自然科學基金資助項目(81602844) 重慶市自然科學基金資助項目(CSTC,2010BB5370)
【分類號】：R714
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本文編號：1883646