本文選題：卵巢上皮性腫瘤 + IL-17A�。� 參考：《天津醫(yī)科大學》2014年碩士論文

【摘要】：目的： 1.檢測IL-17A和IL-17F在卵巢上皮性腫瘤及正常卵巢組織中的表達。 2.分析IL-17A和IL-17F與卵巢上皮性腫瘤各種臨床病理參數(shù)和腫瘤微血管生成的關系,并初步探討其可能機制。 3.評估IL-17A和IL-17F對患者預后的影響。 方法： 1.收集76例惡性卵巢上皮性腫瘤、10例交界性卵巢上皮性腫瘤、10例良性卵巢上皮性腫瘤和10例宮頸癌手術中切除的正常卵巢組織,采用免疫組織化學的方法,觀察IL-17A和IL-17F的表達和分布特征。 2.采用免疫組織化學的方法對血管內(nèi)皮細胞標志物CD105進行染色,計數(shù)惡性卵巢上皮性腫瘤中微血管的生成,探討其與IL-17A、IL-17F表達的相關性。 3.統(tǒng)計分析IL-17A、IL-17F表達與臨床資料的關系,并對隨訪資料進行生存分析。 結果： 1.惡性卵巢上皮性腫瘤組織中IL-17A、IL-17F呈高表達,且IL-17A與CD105(CD105標記血管內(nèi)皮細胞計數(shù)MVD)的表達呈正相關(r=0.326, P0.05), IL-17F與CD105的表達呈負相關(r=-0.232,P0.05)。 2.IL-17A. IL-17F在卵巢癌組織中的表達與組織學分級、有無遠處轉移和有無淋巴結轉移呈正相關(P0.05),而與患者年齡、腫瘤大小、病理分型、有無腹水無相關性(P0.05)。 3.卵巢癌組織中,IL-17A表達陽性組MVD值(4.95±1.91)明顯高于陰性組(3.71±1.87)(P0.05), IL-17F表達陽性組MVD值(4.06-4-1.91)明顯低于陰性組(5.22+2.23)(P0.05)。 4.單因素生存分析顯示,組織學分級Ⅲ-Ⅳ級組平均生存時間較Ⅰ～Ⅱ級組平均生成時間短(P0.05);有遠處轉移組平均生存時間較無遠處轉移組平均生存時間短(P0.05);有淋巴結轉移組平均生存時間較無淋巴結轉移組平均生存時間短(P0.05)；IL-17A高表達組平均生存時間較低表達組平均生存時間短(P0.01)；IL-17F高表達組平均生存時間較低表達組平均生存時間短(P0.01)。多因素生存分析顯示,IL-17A蛋白表達、IL-17F蛋白表達、組織學分級是影響惡性卵巢上皮腫瘤患者預后的獨立因素(P0.05)。 結論： 1. IL-17A、IL-17F在卵巢上皮性腫瘤組織中的表達與腫瘤的惡性程度有關,腫瘤的惡性程度越高,IL-17A和IL-17F表達的陽性率越高。在卵巢上皮性腫瘤的發(fā)生、發(fā)展中起重要作用,可以作為臨床判斷卵巢上皮性腫瘤生物學行為有用的分子生物學指標。 2.IL-17A可發(fā)揮促卵巢癌微血管生成的作用,而IL-17F可發(fā)揮抑制微血管生成的作用,二者通過調(diào)節(jié)促血管生成和抗血管生成的平衡來促進腫瘤微血管的生成,且可作為預測卵巢上皮性腫瘤患者預后的獨立危險因素。
[Abstract]:Objective: 1. The expression of IL-17A and IL-17F in epithelial ovarian tumors and normal ovarian tissues was detected. 2. To analyze the relationship between IL-17A and IL-17F and various clinicopathological parameters and microangiogenesis of ovarian epithelial tumors, and to explore its possible mechanism. 3. To evaluate the influence of IL-17A and IL-17F on the prognosis of patients. Methods: 1. Ten cases of borderline epithelial ovarian tumors and 10 cases of benign ovarian epithelial tumors and 10 cases of normal ovarian tissues were collected from 76 cases of malignant ovarian epithelial tumors and 10 cases of cervical cancer. The expression and distribution of IL-17A and IL-17F were observed. 2. Immunohistochemical staining of vascular endothelial cell marker (CD105) was used to count the formation of microvessels in malignant ovarian epithelial tumors and to explore its correlation with the expression of IL-17An IL-17F. 3. To analyze the relationship between the expression of IL-17 and clinical data, and to analyze the survival of follow-up data. Results: 1. In malignant ovarian epithelial tumors, IL-17An IL-17F expression was highly expressed, and the expression of IL-17A was positively correlated with the expression of CD105(CD105 labeled vascular endothelial cells (MVD) 0.326, P0.05, and IL-17F was negatively correlated with the expression of CD105. 2.IL-17A. The expression of IL-17F in ovarian carcinoma was positively correlated with histological grade, distant metastasis and lymph node metastasis (P 0.05), but had no correlation with age, tumor size, pathological type and ascites. 3. In ovarian cancer, the MVD value of the positive group was 4.95 鹵1.91) and the MVD value of the positive group of IL-17F expression was 4.06-4-1.91), which was significantly higher than that of the negative group (3.71 鹵1.87), and the MVD value of the positive group of IL-17F expression was significantly lower than that of the negative group (5.22 2.23 P 0.05). 4. Univariate survival analysis showed that, Histological grade 鈪，

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