本文選題：子癇前期 + 動物模型　； 參考：《廣西醫(yī)科大學》2014年碩士論文

【摘要】：目的：觀察研究硫酸鎂不同用藥時限治療對子癇前期大鼠模型孕鼠及胎鼠的影響，及對胎腦海馬中微管相關(guān)蛋白2（MAP-2）mRNA、半胱氨酸天冬氨酸蛋白酶3（caspase-3）mRNA表達的影響，及其作用機制。 方法：將確定妊娠的SD大鼠隨機分為正常妊娠對照組、子癇前期模型組、硫酸鎂治療1天組、2天組、4天組，每組6只。于妊娠第14天，除正常妊娠對照組外其余4組孕鼠予低劑量內(nèi)毒素（1.0g/kg）誘導建立子癇前期大鼠模型。治療組分別予硫酸鎂1天、2天、4天不同用藥時限治療，觀察其對孕鼠血壓、尿蛋白的影響。于妊娠第20天剖宮取胎，測量新生鼠身長、體重。再取胎盤組織、胎鼠腦組織固定，觀察各組胎盤HE染色下組織形態(tài)學改變，TUNEL法檢測各組胎腦海馬CA1區(qū)神經(jīng)細胞凋亡情況，通過熒光定量PCR（RT-pPCR）測定各組胎腦海馬中MPA-2mRNA及caspase-3mRNA的表達情況。 結(jié)果：（1）妊娠第15天，除正常妊娠對照組外其余4組孕鼠收縮壓均較自身基礎(chǔ)壓升高30mmHg，差異有統(tǒng)計學意義（P0.05），子癇前期模型組血壓升高并持續(xù)至分娩。予硫酸鎂用藥后，孕期硫酸鎂各治療組大鼠血壓較妊娠第15天明顯降低。妊娠第19天，硫酸鎂用藥2天組、4天組大鼠血壓較模型組明顯降低，差異有統(tǒng)計學意義（P0.05），兩組間比較差異無統(tǒng)計學意義（P0.05）。正常妊娠對照組孕期血壓無明顯變化。（2）妊娠第15天，除正常妊娠對照組外其余4組孕鼠24h尿蛋白水平均高于基礎(chǔ)值2倍以上，與正常妊娠對照組比較，差異有統(tǒng)計學意義（P0.05）。（3）各組胎鼠身長、體重等生長發(fā)育指標比較，差異均無統(tǒng)計學意義（P0.05）。（4）胎盤組織病理學可見子癇前期模型組呈現(xiàn)基帶炎性細胞浸潤、迷路帶組織核碎裂、纖維蛋白沉積等胎盤炎性改變，硫酸鎂各治療組上述病變明顯較輕，各治療組間差異不明顯。（5）TUNLE檢測顯示硫酸鎂治療1天組、2天組、4天組胎腦海馬CA1區(qū)神經(jīng)細胞凋亡數(shù)明顯低于模型組，差異有統(tǒng)計學意義（P0.05）。硫酸鎂治療2天組、4天組較治療1天組明顯改善，，差異有統(tǒng)計學意義（P0.05）。但治療2天組、4天組之間比較無統(tǒng)計學意義（P0.05）。（6）與正常妊娠對照組比較，子癇前期模型組、硫酸鎂治療1天組、2天組、4天組的新生大鼠海馬組織中MAP-2mRNA表達量均降低，差異有統(tǒng)計學意義(P0.05)。硫酸鎂各治療組MAP-2mRNA的表達量較模型組高，差異有統(tǒng)計學意義（P0.01）。硫酸鎂治療2天組、4天組MAP-2mRNA的表達量較硫酸鎂治療1天組高，差異有統(tǒng)計學意義（P0.05）。但硫酸鎂治療2天組、4天組之間比較差異無統(tǒng)計學意義（P0.05）。(7)與正常妊娠對照組比較，子癇前期模型組、硫酸鎂治療1天組、2天組、4天組的新生大鼠海馬組織中caspase-3mRNA表達量均升高，差異有統(tǒng)計學意義(P0.05)。硫酸鎂各治療組caspase-3mRNA的表達量較模型組低，差異有統(tǒng)計學意義（P0.01）。硫酸鎂治療2天組、4天組caspase-3mRNA的表達量較硫酸鎂治療1天組低，差異有統(tǒng)計學意義（P0.05）。但硫酸鎂治療2天組、4天組之間比較差異無統(tǒng)計學意義（P0.05） 結(jié)論：（1）低劑量內(nèi)毒素能成功建立子癇前期大鼠模型，產(chǎn)生高血壓、蛋白尿、胎盤病理學等相應(yīng)表現(xiàn)。（2）硫酸鎂對子癇前期模型孕鼠有一定的療效，表現(xiàn)出間接的降壓作用，并對胎盤功能有一定的改善，無明顯毒副反應(yīng)。（3）硫酸鎂對子癇前期胎腦有一定的治療作用，其保護機制可能與上調(diào)對胎腦MAP2mRNA表達及下調(diào)對caspase-3mRNA表達有關(guān)。（4）硫酸鎂用藥2天組對子癇前期母胎的療效較硫酸鎂用藥1天組、4天組好。
[Abstract]:Objective: To observe and study the effects of different medication time of Magnesium Sulfate on pregnant rats and fetal rats in preeclampsia model, and the effect on the expression of microtubule related protein 2 (MAP-2) mRNA and cysteine aspartic proteinase 3 (caspase-3) mRNA in the hippocampus of fetal brain and its mechanism.
Methods: the SD rats were randomly divided into normal pregnancy control group, preeclampsia model group, Magnesium Sulfate treatment group for 1 days, 2 days group, 4 day group, 6 rats in each group. The other 4 groups of pregnant rats were given low dose endotoxin (1.0g/kg) to establish the preeclampsia rat model except the normal pregnancy control group. The treatment group was given Magnesium Sulfate 1, respectively. 2 days, 2 days, 4 days of different medication time treatment, observe the effect on the blood pressure and urine protein of pregnant rats. On the twentieth day of pregnancy, the body length and body weight of the newborn rats were measured. The placenta tissue and the fetal rat brain were fixed. The histomorphological changes of the placenta in each group were observed by HE staining. The apoptosis of the hippocampal CA1 area of the fetal brain was detected by TUNEL method. The expression of MPA-2mRNA and caspase-3mRNA in the hippocampus of fetal brain of each group was detected by fluorescent quantitative PCR (RT-pPCR).
Results: (1) on the fifteenth day of pregnancy, the systolic pressure of the other 4 groups of pregnant rats except the normal pregnancy control group increased by 30mmHg. The difference was statistically significant (P0.05). The blood pressure of the preeclampsia model group increased and continued to labor. After Magnesium Sulfate, the blood pressure of the rats in each group of pregnant Magnesium Sulfate was significantly lower than that of the pregnancy. The pregnancy rate was nineteenth. The pregnancy rate was nineteenth. In the 2 day group of Magnesium Sulfate, the blood pressure of the 4 day group was significantly lower than that of the model group (P0.05). There was no significant difference between the two groups (P0.05). (2) the fifteenth days of pregnancy, the other 4 groups of pregnant rats except the normal pregnancy control group were higher than the basic value 2. Compared with the normal pregnancy control group, the difference was statistically significant (P0.05). (3) there was no significant difference in the growth and development indexes of fetal rats, such as body length and weight (P0.05). (4) placental histopathology showed that the model group of preeclampsia presented basic inflammatory cell infiltration, labyrinthine tissue fragmentation, fibrin deposition and other placenta The pathological changes in the Magnesium Sulfate treatment group were lighter, and the difference between the treatment groups was not obvious. (5) TUNLE detection showed that the number of neuronal apoptosis in the CA1 region of the fetal brain hippocampus was significantly lower than that of the model group in the 1 day group, the 2 day group and the 4 day group, the difference was statistically significant (P0.05). The 4 day group was significantly improved than the 1 day group. The difference was statistically significant (P0.05), but there was no significant difference between the 2 days group and the 4 day group (P0.05). (6) compared with the normal pregnancy control group, the preeclampsia model group, the 1 day Magnesium Sulfate treatment group, the 2 day group, the 4 day group of newborn rats were all decreased in the hippocampus, the difference was statistically significant (P0.05). Each treatment was statistically significant (P0.05). The expression of MAP-2mRNA was higher than that of the model group (P0.01). The expression of MAP-2mRNA in the 2 day group was higher than that in the 4 day group, and the difference was statistically significant (P0.05). However, there was no significant difference between the 2 days group of Magnesium Sulfate treatment and the 4 day group (P0.05). (7) compared with the normal pregnancy control group, the difference was not statistically significant (P0.05). In the preeclampsia model group, the expression of caspase-3mRNA in the hippocampus of the 1 day group, the 2 day group and the 4 day group was increased, and the difference was statistically significant (P0.05). The expression of caspase-3mRNA in each group of Magnesium Sulfate was lower than that of the model group (P0.01). The expression of caspase-3mRNA in the 4 day group of Magnesium Sulfate treatment and the expression of caspase-3mRNA in the 4 day group were expressed. The difference was statistically significant (P0.05), but there was no significant difference between the 2 day group and the 4 day group (P0.05). The difference between the 1 groups was statistically significant (P0.05).
Conclusions: (1) low dose endotoxin can successfully establish preeclampsia rat model and produce hypertension, proteinuria and placental pathology. (2) Magnesium Sulfate has a certain effect on the preeclampsia model pregnant rats, showing indirect antihypertensive effect, and having some improvement on placental function and no obvious side effects. (3) Magnesium Sulfate before epilepsy. The protective mechanism of fetal brain may be related to the up regulation of MAP2mRNA expression and down regulation of caspase-3mRNA in fetal brain. (4) the effect of the 2 day Magnesium Sulfate drug group on the preeclampsia mother fetus is better than that of the 1 day group of Magnesium Sulfate.

【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R714.244

【參考文獻】

相關(guān)期刊論文 前3條

1 王林;陳必良;辛曉燕;楊紅;張俊茹;張敏;;硫酸鎂對輕度子癇前期療效的隨機對照臨床試驗[J];山西醫(yī)科大學學報;2007年08期

2 夏革清;徐丹芬;吳敏;吳超英;;Expression of Toll-like Receptor 4 in Neonatal Cord Blood Mononuclear Cells in Patients with Preeclampsia[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2010年05期

3 吳文英;徐春琳;李海霞;王玉紅;;早產(chǎn)兒出生前硫酸鎂暴露量與腦損傷關(guān)系的研究[J];中國優(yōu)生與遺傳雜志;2008年05期

本文編號：1881711