本文選題：Hh信號通路 + 缺氧��； 參考：《延邊大學(xué)》2014年碩士論文

【摘要】：目的 缺氧條件下觀察宮頸癌Hela中Hedgehog (Hh)信號通路相關(guān)因子和腫瘤干細(xì)胞標(biāo)志物的表達(dá)及球形體形成能力。 方法 宮頸癌Hela細(xì)胞加CoC12分別培養(yǎng)24h和48h后,采用定量RT-PCR法和Western-Blot方法檢測缺氧誘導(dǎo)因子(HIF1α和HIF2α)、Hh信號通路相關(guān)因子(smo、Gli-1)和腫瘤干細(xì)胞標(biāo)志物(oct-4, sox2, Nanog)的表達(dá)；采用球形體形成實(shí)驗(yàn)檢查Hela細(xì)胞的集落形成能力。 結(jié)果 宮頸癌He1a細(xì)胞的HIF1α與HIF2α的表達(dá)量,在缺氧24h開始增加,隨著時間的延長,其表達(dá)量明顯增加。Smo和Gli-1的mRNA量在缺氧48h開始增加；72h時Smo量是常氧組的5.4倍(p0.01,p0.01),Gli-1量是常氧組的123倍(p0.001,p0.001)。腫瘤干細(xì)胞(CSCs)標(biāo)志物mRNA量在缺氧24h時開始增高,隨著缺氧時間的延長,其表達(dá)量增加明顯；72h時0ct4量是常氧組的78.8倍,Sox2量是常氧組的787.4倍,Nanog量是常氧組的689.8倍(p0.001,p0.001,p0.001)。缺氧組形成球形體的能力明顯高于常氧組(p0.01)。 結(jié)論 在缺氧條件下Hela細(xì)胞中Hedgehog信號通路被激活,并且誘導(dǎo)Hela細(xì)胞具有腫瘤干細(xì)胞性。
[Abstract]:Purpose The expression of Hedgehog Hhh signal pathway and the expression of tumor stem cell markers and the ability of spheroid formation in Hela were observed under hypoxia condition. Method After Hela cells were cultured with CoC12 for 24 h and 48 h respectively, the expression of HIF1 偽 and HIF2 偽 Hh signaling pathway related factors (Gli-1) and tumor stem cell markers (oct-4, sox2, Nanog-1) were detected by quantitative RT-PCR and Western-Blot. The colony forming ability of Hela cells was examined by spheroid formation test. Result The expression of HIF1 偽 and HIF2 偽 in He1a cells of cervical cancer began to increase at 24 h of hypoxia. With the prolongation of time, the expression of mRNA in Smo and Gli-1 was significantly increased at 48 h after hypoxia, and the Smo level was 5.4 times that in normoxic group at 72 h after hypoxia, and the amount of Gli-1 was 123 times of that in normoxic group (p 0.001, p0.001, p0.001). The amount of mRNA, a tumor stem cell mRNA marker, began to increase at 24 h after hypoxia. With the prolongation of hypoxia time, the expression of 0ct4 was 78.8-fold higher than that of normoxic group at 72 h. The amount of 0ct4 was 787.4 times higher than that of normoxic group and 689.8 times of that of normoxic group (P 0.001p0.001p0.001p0.001). The ability to form spheroids in hypoxia group was significantly higher than that in normoxic group (P 0.01). Conclusion Under hypoxia condition, Hedgehog signaling pathway was activated in Hela cells, and Hela cells were induced to be tumor-stem cells.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R737.33

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