本文選題：宮頸管分泌物 + 細(xì)菌培養(yǎng)��； 參考：《山西醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的比較宮頸不同處理方法對(duì)宮頸管分泌物細(xì)菌培養(yǎng)結(jié)果的影響。 方法將細(xì)菌性陰道病、陰道清潔度III-IV度的盆腔炎性疾病患者80例,按照宮頸外口處理方法不同分為五組。A組：用無(wú)菌拭子經(jīng)宮頸外口進(jìn)入宮頸管取分泌物；B組：無(wú)菌干棉球擦拭宮頸外口后取材；C組：生理鹽水、無(wú)菌干棉球先后擦拭宮頸外口后取材；D組：0.5%碘伏擦拭宮頸外口后取材；E組：0.5%碘伏、生理鹽水、無(wú)菌干棉球依次擦拭宮頸外口后取材。每組均同時(shí)取陰道分泌物行細(xì)菌培養(yǎng)。根據(jù)菌落的不同,挑取不同菌落行革蘭氏染色,進(jìn)行菌種的識(shí)別,計(jì)數(shù)培養(yǎng)基中菌種數(shù)。對(duì)不同方法處理宮頸外口后宮頸管細(xì)菌培養(yǎng)菌種數(shù)比較,配對(duì)比較宮頸管與陰道分泌物細(xì)菌培養(yǎng)菌種數(shù)。 結(jié)果A組與陰道分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異無(wú)統(tǒng)計(jì)學(xué)意義(5.31±1.62,6.06±0.68,P0.05)；B組與陰道分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異有統(tǒng)計(jì)學(xué)意義(3.88±1.09,6.19±0.66,P0.001)；C組與陰道分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異有統(tǒng)計(jì)學(xué)意義(3.38±1.20,6.06±0.57,P0.001)；D組與陰道分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異有統(tǒng)計(jì)學(xué)意義(0.69±0.48,6.06±0.44,P0.001);E組與陰道分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異有統(tǒng)計(jì)學(xué)意義(3.25±0.45,6.13±0.62,P0.001)。五組陰道分泌物細(xì)菌培養(yǎng)菌種數(shù)差異無(wú)統(tǒng)計(jì)學(xué)意義(F=0.139,P=0.967)。A組與其它四組宮頸管分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異均有統(tǒng)計(jì)學(xué)意義(P0.05)；B組與C組宮頸管分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),與D組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),與E組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)；C組與D組宮頸管分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),與E組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)；D組與E組宮頸管分泌物細(xì)菌培養(yǎng)菌種數(shù)比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論取宮頸管分泌物細(xì)菌培養(yǎng)前有必要對(duì)宮頸外口進(jìn)行預(yù)處理；對(duì)宮頸外口進(jìn)行簡(jiǎn)單的處理如無(wú)菌干棉球擦拭,就可以降低陰道微生物的污染,同時(shí)簡(jiǎn)化了取材前宮頸外口處理方法。
[Abstract]:Objective to compare the effect of different treatment methods on bacterial culture of cervical secretion. Methods 80 patients with bacterial vaginosis and pelvic inflammatory disease with III-IV degree of vaginal cleanliness were studied. According to the different methods of external cervix treatment, group A was divided into five groups: group B: sterilized swab was used to enter the cervix canal through cervix to take secretion from cervical canal, group B: group C: normal saline after wiping cervix external mouth with aseptic dry cotton ball. Sterile dry cotton balls were used to wipe the external cervix of cervix. Group D: 0.5% iodophor was taken after wiping cervical external mouth. Group E: 0.5% iodophor, physiological saline, sterile dry cotton ball were used to wipe the material after cervical external mouth in turn. Vaginal secretions were taken from each group at the same time for bacterial culture. According to the different colonies, different colonies were picked up for Gram staining to identify the species and count the number of strains in the culture medium. The number of bacteria cultured in the cervical canal treated with different methods was compared, and the number of bacteria cultured in the cervical canal and vaginal secretion was compared. Results there was no significant difference between group A and group A in the number of bacteria cultured in vaginal secretions. There was no significant difference between group A and group B in the number of bacteria cultured in vaginal secretions. There was a significant difference between group B and group B in the number of bacteria cultured in vaginal secretions. There was a significant difference between group C and group C in the number of bacteria cultured in vaginal secretions. There was significant difference between group D (3.38 鹵1.206.06 鹵0.57p 0.001) and vaginal secretion (P 0.001). There was significant difference between group E and group E (0.69 鹵0.48U, 6.06 鹵0.44P 0.001) and group E (3.25 鹵0.456.13 鹵0.62P 0.001). There was no significant difference in the number of bacteria cultured in vaginal secretions among the five groups. There were significant differences between group A and the other four groups in the number of bacteria cultured in cervical secretions. There were significant differences in the number of bacteria cultured in cervical secretions between group A and group C. there were significant differences between group A and group C in the number of bacteria cultured in cervical secretions. There was no significant difference in the number of bacteria in cervical secretion between group C and group D, but there was no significant difference between group D and group D, and there was no significant difference between group E and group C in the number of bacteria cultured in cervical canal secretion. There was a significant difference between group C and group D in the number of bacteria cultured in cervical canal secretion. There was no significant difference between group D and group E in the number of bacteria cultured in the secretion of cervical canal. There was significant difference between group D and group E in the number of bacteria cultured in the secretion of cervical canal (P0.05). Conclusion it is necessary to pretreat the external cervix orifice before the bacterial culture of the cervical canal secretions, and the simple treatment of the external cervix orifice, such as wiping the sterile dry cotton ball, can reduce the contamination of the vaginal microorganism. At the same time, it simplifies the treatment method of cervical external orifice before sampling.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R711.3

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