本文選題：子宮內(nèi)膜腫瘤 + 飛行時間質(zhì)譜��； 參考：《中華腫瘤防治雜志》2015年11期

【摘要】：目的研究子宮內(nèi)膜癌患者血清多肽譜的變化,探討子宮內(nèi)膜癌早期診斷相關(guān)的差異蛋白。方法選取2008-05-01-2011-05-31浙江省腫瘤醫(yī)院收治的子宮內(nèi)膜癌患者134例、子宮體良性病變患者103例和健康女性志愿者95名作為研究對象。將研究對象分為測試組(45例Ⅰ期子宮內(nèi)膜癌患者和45名健康女性)、驗證組(134例子宮內(nèi)膜癌患者和95名健康女性),采用基體輔助激光解吸電離飛行時間質(zhì)譜(matrix-associated laser disso-ciation/ionization time of flight mass spectrmtry,MALDI-TOF-MS)結(jié)合納米磁珠技術(shù)測定其血清多肽譜,Biomarker Wizard軟件篩選差異蛋白峰,采用SPSS 12.0中ROC曲線分析差異峰建立診斷模型,進(jìn)一步評價模型對診斷子宮內(nèi)膜癌的敏感度和特異性,并分析差異蛋白峰在各期子宮內(nèi)膜癌及子宮良性病變患者中的分布情況。結(jié)果血清多肽譜檢測結(jié)果顯示,測試組質(zhì)荷比為1 500~30 000m/z,共測得82個蛋白峰,其中有12個差異峰差異有統(tǒng)計學(xué)意義,P0.001。在12個差異峰中,取ROC曲線面積≥0.8的2 768、3 402和6 441m/z 3個差異峰作為子宮內(nèi)膜癌的診斷模型,其敏感度分別為78.4%、75.4%和66.8%,特異性分別為92.6%、89.5%和86.2%;三者聯(lián)合的敏感度和特異性為88.1%和75.8%。聯(lián)合診斷模型鑒別子宮內(nèi)膜癌和子宮良性病變的敏感度為94.9%。結(jié)論血清多肽譜檢測篩查發(fā)現(xiàn)的差異峰2 768、3 402和6 441m/z能較正確的區(qū)分子宮內(nèi)膜癌患者、健康人和子宮良性病變患者,有助于子宮內(nèi)膜癌的早期篩查。
[Abstract]:Objective to study the changes of serum polypeptide spectrum in patients with endometrial carcinoma and to explore the differential proteins associated with early diagnosis of endometrial carcinoma. Methods 134 cases of endometrial carcinoma, 103 cases of uterine body benign lesion and 95 healthy female volunteers were selected from Zhejiang Provincial Cancer Hospital from January to 31, 2008- 05-01-2011-05-31. The subjects included 134 patients with endometrial carcinoma, 103 patients with benign uterine body disease and 95 healthy women volunteers. The subjects were divided into two groups: 45 patients with stage I endometrial carcinoma and 45 healthy women, and 134 patients with endometrial carcinoma and 95 healthy women in the test group. Matrix-associated laser disso-ciation/ionization was used in matrix assisted laser desorption ionization time of flight mass spectrometry. Time of flight mass spectrum MALDI-TOF-MS combined with nano-magnetic bead technique was used to determine the serum polypeptide spectrum and Biomarker Wizard software to screen the differential protein peaks. The differential peak of SPSS 12.0 was used to establish a diagnostic model to evaluate the sensitivity and specificity of the model in the diagnosis of endometrial carcinoma, and to analyze the distribution of differential protein peak in patients with endometrial carcinoma and benign lesions. Results the results of serum polypeptide spectrum showed that the mass charge ratio of the tested group was 1 500 ~ 30 000 m / z, and 82 protein peaks were detected, of which 12 of them showed significant difference (P 0.001). Among the 12 differential peaks, the sensitivity and specificity of the three peaks with ROC curve area 鈮，

本文編號：1850741