本文選題：圍著床期 + 子宮　； 參考：《南方醫(yī)科大學(xué)學(xué)報(bào)》2015年03期

【摘要】：目的觀察小鼠子宮中胚胎著床部位與非著床部位巨噬細(xì)胞(Macrophage,Mφ)的分布差異,探討其在胚胎著床過程中的作用。方法取真孕(n=30)和假孕小鼠(n=30)子宮組織,選擇30只妊娠小鼠模型作為實(shí)驗(yàn)組,同時(shí)選擇30只假孕小鼠模型作為對照組,在圍著床期取兩組小鼠子宮組織,采用免疫組化技術(shù)檢測子宮組織中Mφ、誘導(dǎo)型一氧化氮合成酶(i NOS)、白血病抑制因子(LIF)的分布,分析比較子宮組織中胚胎著床部位和非著床部位Mφ、i NOS和LIF的分布差異及相關(guān)性。結(jié)果在小鼠子宮組織中,觀察到實(shí)驗(yàn)組Mφ在D4.5(雌鼠交配后次日晨陰道見栓為D0.5)前主要分布子宮內(nèi)膜,D4.5后主要分布于子宮外膜,對照組Mφ主要分布于子宮內(nèi)膜;實(shí)驗(yàn)組i NOS在D4.5前主要分布子宮內(nèi)膜,D4.5后主要見于子宮肌膜和外膜,對照組i NOS主要位于子宮內(nèi)膜,且實(shí)驗(yàn)組Mφ和i NOS分布呈正相關(guān)。此外,實(shí)驗(yàn)組LIF顯著表達(dá)于子宮內(nèi)膜,對照組幾乎不表達(dá),二者間有統(tǒng)計(jì)學(xué)差異(P0.05);著床部位Mφ、i NOS和LIF明顯高于非著床部位,其中Mφ、LIF比較有統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論圍著床期小鼠子宮組織中Mφ主要分布在子宮內(nèi)膜及胚胎著床點(diǎn),并伴有i NOS和LIF的表達(dá),提示Mφ在胚胎著床中可能發(fā)揮重要作用。
[Abstract]:Objective to observe the distribution of Macrophagege M 蠁 (M 蠁) in mouse uterus and to explore the role of M 蠁 in embryo implantation. Methods the uterine tissues of the two groups were selected, 30 pregnant mice as experimental group and 30 pseudopregnant mice as control group. The uterine tissues of the two groups were taken during the period of surrounding bed. The distribution of M 蠁, inducible nitric oxide synthase (iNOS) and leukemia inhibitory factor (LIF) in uterine tissues were detected by immunohistochemical technique. The difference and correlation of M 蠁 NOS and LIF in embryo implantation and non-implantation were analyzed and compared. Results in the uterine tissues of mice, M 蠁 was mainly distributed in the endometrium before D4.5 (the vaginal thrombus was D0.5 in the morning after mating) in the experimental group, and mainly in the outer membrane after D4.5 in the control group, while in the control group, M 蠁 was mainly distributed in the endometrium. I NOS was mainly distributed in the myometrium and adventitia after D4.5 in the experimental group, and I NOS in the control group was mainly located in the endometrium, and the distribution of M 蠁 and I NOS in the experimental group was positively correlated. In addition, the expression of LIF was significantly higher in the experimental group than in the control group, and there was a significant difference between the two groups (P 0.05), and the M 蠁 I NOS and LIF in the implantation site were significantly higher than those in the non-implantation site, and there was a significant difference between the two groups (P 0.05). Conclusion M 蠁 mainly distributes in endometrium and embryo implantation site, accompanied by the expression of I NOS and LIF, suggesting that M 蠁 may play an important role in embryo implantation.
【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院婦產(chǎn)科生殖醫(yī)學(xué)中心;
【基金】：國家自然科學(xué)基金(81170575) 廣東省自然科學(xué)基金(9151051501000066)~~
【分類號(hào)】：R714.8

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本文編號(hào)：1849450