本文選題：大鼠 + 子宮內(nèi)膜異位癥模型　； 參考：《實(shí)用婦產(chǎn)科雜志》2017年04期

【摘要】：目的:觀察縮宮素對(duì)大鼠子宮內(nèi)膜異位癥(EMT)模型的治療作用。方法:建模成功的SD大鼠隨機(jī)分為縮宮素(OT)組和0.9%氯化鈉液(NS)組各20只,OT組采用肌內(nèi)注射OT 160μg/kg·d~(-1),NS組采用肌內(nèi)注射0.9%氯化鈉液0.1 ml/d。治療4周后開腹觀察異位病灶體積變化,應(yīng)用RT-PCR、Western-blot檢測治療前后兩組大鼠異位內(nèi)膜組織中血管內(nèi)皮生長因子(VEGF)、腫瘤壞死因子α(TNF-α)、血小板反應(yīng)素-1(TSP-1)mRNA及蛋白的表達(dá)水平。結(jié)果:(1)治療前,OT組大鼠異位病灶體積(40.61±9.45 mm~3)與NS組(39.32±7.75 mm~3)比較,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。治療4周后,OT組大鼠異位病灶體積(15.21±3.11 mm~3)較NS組(39.59±7.65 mm~3)明顯減小(P0.05)。NS組治療前后異位病灶體積無明顯變化(P0.05)。(2)OT組VEGF mRNA、TNF-αmRNA相對(duì)表達(dá)量(0.21±0.02,0.41±0.20)均較NS組(0.87±0.05,1.09±0.10)明顯下降(P0.05);OT組TSP-1 mRNA的相對(duì)表達(dá)量(3.87±0.84)較NS組(0.96±0.25)明顯升高(P0.05)。(3)OT組VEGF及TNF-α蛋白相對(duì)表達(dá)量(0.12±0.17,0.17±0.20)均較NS組(1.34±0.35,1.07±0.23)明顯下降(P0.05);OT組TSP-1蛋白相對(duì)表達(dá)量(1.01±0.14)較NS組(0.33±0.07)明顯升高(P0.05)。結(jié)論:縮宮素對(duì)大鼠EMT模型可能具有治療作用,EMT病灶組織的高度血管化,可能與VEGF、TNF-α和TSP-1的表達(dá)失衡密切相關(guān)。
[Abstract]:Objective: to observe the therapeutic effect of oxytocin on rat model of endometriosis. Methods: SD rats were randomly divided into oxytocin group (n = 20) and 0.9% sodium chloride (NS) group (n = 20). Each group was given intramuscular injection of OT160 渭 g/kg / d ~ (-1) and intramuscular injection of 0.9% sodium chloride solution (0.1 ml 路d ~ (-1) 路d ~ (-1). After 4 weeks of treatment, the volume changes of ectopic lesions were observed. The expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor- 偽 (TNF- 偽), platelet-reactivin-1 (TSP-1) mRNA and protein in ectopic endometrium of rats before and after treatment were detected by RT-PCR- Western-blot. Results before treatment, the volume of ectopic lesions in the OT group was 40.61 鹵9.45 mm ~ (-3), which was not significantly different from that in the NS group (39.32 鹵7.75 mm ~ (-3). 4 weeks after treatment, the volume of ectopic focus in the OT group was 15.21 鹵3.11 mm ~ (3) significantly lower than that in the NS group (39.59 鹵7.65 mm ~ (-3). The volume of the ectopic lesion in the control group did not change significantly before and after treatment. The relative expression of VEGF mRNA-TNF- 偽 mRNA in the OT group was 0.21 鹵0.022 鹵0.41 鹵0.20), which was significantly lower than that in the NS group (0.87 鹵0.051.09 鹵0.10). The relative expression of VEGF and TNF- 偽 protein was significantly increased in the group of P0.05T and TNF- 偽 (0.12 鹵0.170.17 鹵0.20) than that in the group of NS (1.34 鹵0.35 鹵0.07 鹵0.23). The relative expression of TSP-1 protein in P0.05OT group was 1.01 鹵0.14) significantly higher than that in the NS group (0.33 鹵0.07). Conclusion: oxytocin may play a therapeutic role in the treatment of EMT in rats. It may be closely related to the imbalance of expression of VEGF TNF- 偽 and TSP-1.
【作者單位】： 南通大學(xué)第二附屬醫(yī)院;南通大學(xué)實(shí)驗(yàn)動(dòng)物中心;
【基金】：基金項(xiàng)目:南通市科技局重點(diǎn)病種的規(guī)范化臨床診療研究(編號(hào):HS2014074)
【分類號(hào)】：R-332;R711.71

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