本文選題：子癇前期 + FoxO4　； 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的：通過研究FoxO4在子癇前期患者蛻膜中表達(dá)的變化,分析其在蛻膜血管急性動(dòng)脈粥樣硬化形成中的作用,并闡明其與母體氧化應(yīng)激反應(yīng)的關(guān)系,從而探討FoxO4如何參與了子癇前期這一疾病的發(fā)生發(fā)展。 方法：選取2013年7月～2014年3月在中南大學(xué)湘雅二醫(yī)院確診為“子癇前期”的25例孕婦作為研究組,重度子癇前期15例,輕度子癇前期10例；選取同期正常妊娠的15例孕婦作為對(duì)照組,兩組孕婦在分娩未發(fā)動(dòng)之前均以剖宮產(chǎn)終止妊娠。將40例患者蛻膜組織切片行HE染色,根據(jù)HE染色結(jié)果再次進(jìn)行分組。采用酶聯(lián)免疫吸附試驗(yàn)(ELISA)分別檢測(cè)各組孕婦血清中8-異前列腺素蛋白濃度,并進(jìn)行統(tǒng)計(jì)學(xué)分析；采用免疫印跡法(Western Blot)分別檢測(cè)各組孕婦蛻膜中8-異前列腺素、FoxO4及磷酸化FoxO4(P-FoxO4)的蛋白表達(dá)水平,并進(jìn)行統(tǒng)計(jì)學(xué)分析,利用免疫組化方法檢測(cè)各組蛻膜中的P-FoxO4蛋白表達(dá)定位。同時(shí)進(jìn)一步分析了重度子癇前期、輕度子癇前期及正常對(duì)照組中蛻膜FoxO4、P-Foxo4,血清及蛻膜中8-異前列腺素的表達(dá)差異。 結(jié)果：1.子癇前期患者蛻膜中FoxO4及磷酸化FoxO4較正常對(duì)照組升高,且重度組高于輕度組；同時(shí)發(fā)現(xiàn)子癇前期患者血清及蛻膜中8-異前列腺素較正常對(duì)照組升高,且重度組高于輕度組。2.蛻膜HE染色顯示：子癇前期患者中蛻膜血管“急“性動(dòng)脈粥樣硬化病變”發(fā)生率為40%,正常對(duì)照組患者蛻膜血管均無(wú)病變。3.依此將患者分為子癇前期伴有蛻膜血管“急性動(dòng)脈粥樣硬化”組(PE+Aa);子癇前期不伴蛻膜血管“急性動(dòng)脈粥樣硬化”組(PE-Aa);正常對(duì)照組(NP)。4.三組孕婦蛻膜中FoxO4蛋白相對(duì)灰度值呈下降趨勢(shì)(P0.01)：PE+Aa組0.63±0.10；PE-Aa組0.48±0.07；NP組0.33±0.04。5.三組孕婦蛻膜中P-FoxO4蛋白相對(duì)灰度值呈下降趨勢(shì)(P0.01)：PE+Aa組0.50±0.09：PE-Aa組0.40±0.06；NP組0.26±0.04。6.PE+Aa組孕婦蛻膜中存在大量表達(dá)P-FoxO4的蛻膜細(xì)胞,且明顯多于PE-Aa組及NP組。7.三組孕婦血清中8-異前列腺素濃度呈下降趨勢(shì)(P0.01)：PE+Aa組338.1±27.5pg/ml; PE-Aa組250.8±30.1pg/ml; NP組155.6±11.0pg/ml。8.三組孕婦蛻膜中8-異前列腺素蛋白相對(duì)灰度值呈下降趨勢(shì)(P0.01)：PE+Aa組0.53±0.07；PE-Aa組0.41±0.09；NP組0.31±0.07。9.三組孕婦蛻膜組織中FoxO4及P-FoxO4的蛋白水平均與蛻膜組織及外周血中8-異前列腺素蛋白水平呈正相關(guān)關(guān)系。 結(jié)論：1.重度子癇前期孕婦蛻膜中FoxO4、P-FoxO4及8-異前列腺素的表達(dá)均較輕度組及正常組增高。且重度子癇前期患者中,蛻膜血管“急性動(dòng)脈粥樣硬化”病變發(fā)生率較輕度組及正常組升高。2.在子癇前期孕婦中,有蛻膜血管“急性動(dòng)脈粥樣硬化”組的FoxO4, P-FoxO4及8-異前列腺素表達(dá)水平較無(wú)蛻膜血管“急性動(dòng)脈粥樣硬化”組升高。3.FoxO4可能通過調(diào)控蛻膜血管“急性動(dòng)脈粥樣硬化”的形成,參與推動(dòng)了子癇前期孕婦局部及全身的氧化應(yīng)激失衡。
[Abstract]:Objective: to study the expression of FoxO4 in decidua of preeclampsia patients, to analyze its role in the formation of acute atherosclerosis of decidual vessels, and to elucidate the relationship between the expression of FoxO4 and maternal oxidative stress. To explore how FoxO4 is involved in the development of preeclampsia. Methods: from July 2013 to March 2014, 25 pregnant women diagnosed as "preeclampsia" in Xiangya No.2 Hospital of Central South University were selected as study group. 15 cases were severe preeclampsia and 10 cases were mild preeclampsia. 15 pregnant women with normal pregnancy were selected as control group. The pregnant women in both groups were terminated by cesarean section before delivery. The decidua sections of 40 patients were stained by HE staining and grouped according to the results of HE staining. Serum 8-isoprostaglandin protein (8-isoprostaglandin) concentration in pregnant women in each group was detected by enzyme linked immunosorbent assay (Elisa) and analyzed statistically. The expression levels of 8-isoprostaglandin FoxO4 and phosphorylated FoxO4 P-FoxO4 in decidua of pregnant women were detected by Western blot. The expression of P-FoxO4 protein in decidua of each group was detected by immunohistochemical method. At the same time, we further analyzed the difference of the expression of 8-isoprostaglandin in the serum and decidua of severe preeclampsia, mild preeclampsia and normal control group. The result is 1: 1. The levels of FoxO4 and phosphorylated FoxO4 in decidua of preeclampsia patients were higher than those in normal controls, and the levels of 8-isoprostaglandin in serum and decidua of patients with preeclampsia were higher than those in mild eclampsia, and those in severe group were higher than those in mild group. He staining of decidua showed that the incidence of decidual vascular "acute" atherosclerosis was 40 in preeclampsia patients, while that in normal control group was not. 3. The patients were divided into preeclampsia with decidual "acute atherosclerosis" group, preeclampsia without decidual vessel "acute atherosclerosis" group and normal control group with NP.4. The relative gray value of FoxO4 protein in decidua of three groups showed a decreasing trend: P0.01: PE-Aa group 0.63 鹵0.10 PE-Aa group 0.48 鹵0.07 PE-Aa group 0.33 鹵0.04.5 in NP group. The relative gray value of P-FoxO4 protein in decidua of the three groups showed a decreasing trend. There were a large number of decidua cells expressing P-FoxO4 in decidua of pregnant women in the group of 0.50 鹵0.09:PE-Aa 0.40 鹵0.06NP-0.26 鹵0.04.6.PE AA, which were significantly more than those in group PE-Aa and group NP. The concentration of 8-isoprostaglandin in serum of pregnant women in the three groups showed a decreasing trend: P0.01A group (338.1 鹵27.5 PG / ml); PE-Aa group (250.8 鹵30.1pg / ml); NP group (155.6 鹵11.0pg / ml 路8pg / ml). The relative gray value of 8-isoprostaglandin protein in decidua of the three groups showed a decreasing trend: P0.01: PEAa 0.53 鹵0.07 PE-Aa 0.41 鹵0.09 PE-Aa 0.41 鹵0.09 NP 0.31 鹵0.07.9. The protein levels of FoxO4 and P-FoxO4 in decidua tissue were positively correlated with 8-isoprostaglandin protein level in decidua tissue and peripheral blood of the three groups. Conclusion 1. The expression of FoxO4 P-FoxO4 and 8-isoprostaglandin in decidua of pregnant women with severe preeclampsia was higher than that of mild group and normal group. In patients with severe preeclampsia, the incidence of decidual vascular "acute atherosclerosis" was higher than that of mild and normal groups. Among pregnant women with preeclampsia, The expression levels of FoxO4, P-FoxO4 and 8-isoprostaglandin in decidual vessel "acute atherosclerosis" group were higher than those without decidual vessel "acute atherosclerosis" group. 3. FoxO4 may regulate the formation of decidual vascular "acute atherosclerosis". Participation promotes local and systemic oxidative stress imbalance in preeclampsia pregnant women.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714.244

【共引文獻(xiàn)】

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本文編號(hào)：1831409