本文選題：子宮頸腫瘤 + 子宮頸上皮內(nèi)病變　； 參考：《臨床與實(shí)驗(yàn)病理學(xué)雜志》2017年09期

【摘要】：目的探討SIX1在子宮頸癌組織中的表達(dá)以及生物學(xué)作用。方法采用免疫組化Eli Vision兩步法檢測(cè)35例子宮頸癌、42例子宮頸上皮內(nèi)病變(cervical intraepithelial neoplasia,CIN)及15例正常子宮頸組織中SIX1的表達(dá)及Ki-67增殖指數(shù)。應(yīng)用RNA干擾技術(shù),采用SIX1-siRNA表達(dá)質(zhì)粒轉(zhuǎn)染子宮頸癌Hela細(xì)胞,檢測(cè)轉(zhuǎn)染后子宮頸癌Hela細(xì)胞SIX1的mRNA和蛋白表達(dá)水平;應(yīng)用MTT檢測(cè)Hela細(xì)胞的增殖能力。結(jié)果子宮頸癌組織中SIX1陽(yáng)性率高于CIN組織及正常子宮頸組織(P0.05),且與Ki-67增殖指數(shù)呈正相關(guān)(P0.05)。RT-PCR和Western blot檢測(cè)顯示轉(zhuǎn)染SIX1-siRNA子宮頸癌Hela細(xì)胞中SIX1在mRNA和蛋白水平均顯著下調(diào);Hela細(xì)胞生長(zhǎng)速度減慢。結(jié)論 SIX1可能通過(guò)促進(jìn)子宮頸癌細(xì)胞的增殖,在子宮頸癌的發(fā)生、發(fā)展過(guò)程中發(fā)揮重要作用。
[Abstract]:Objective to investigate the expression and biological effect of SIX1 in cervical carcinoma. Methods immunohistochemical Eli Vision two step method was used to detect 35 cases of cervical cancer, 42 cases of cervical intraepithelial lesion (cervical intraepithelial neoplasia, CIN) and 15 cases of normal cervical tissues and Ki-67 proliferation index. RNA interference technique was used. The transfection of Hela cells with SIX1-siRNA expression plasmid was used to detect the mRNA and protein expression level of SIX1 in Hela cells of cervical cancer after transfection. The proliferation ability of Hela cells was detected by MTT. The positive rate of SIX1 in cervical carcinoma tissue was higher than that of CIN tissue and normal cervical tissue (P0.05), and there was a positive correlation with Ki-67 proliferation index (P0.05). CR and Western blot tests showed that SIX1 in Hela cells transfected with SIX1-siRNA uterine cervix significantly decreased in mRNA and protein levels, and the growth rate of Hela cells slowed down. Conclusion SIX1 may play an important role in the development of cervical cancer by promoting the proliferation of cervical cancer cells.

【作者單位】： 秦皇島市第一醫(yī)院病理科;
【分類(lèi)號(hào)】：R737.33

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本文編號(hào)：1814882