本文選題：紫杉醇 + 耐藥　； 參考：《腫瘤防治研究》2015年04期

【摘要】：目的研究紫杉醇誘導(dǎo)的卵巢癌耐藥細(xì)胞株的生物學(xué)特性,探索耐藥細(xì)胞的長期保存條件,以建立穩(wěn)定的體外實驗?zāi)Ｐ�。方法選用卵巢癌化療敏感細(xì)胞株SKOV3,分別采用大劑量沖擊和小劑量濃度遞增誘導(dǎo)建立耐藥的卵巢癌細(xì)胞株SKOV3/TAX300、SKOV3/TAX30。四甲基偶氮唑鹽(MTT)法檢測耐藥細(xì)胞對化療藥物紫杉醇的IC50;比較耐藥細(xì)胞和敏感細(xì)胞的細(xì)胞平板克隆形成、生長曲線倍增時間的差異;熒光定量PCR檢測敏感細(xì)胞和耐藥細(xì)胞中耐藥相關(guān)基因的表達(dá);比較無藥凍存和加藥凍存的方式對耐藥細(xì)胞的保存效果。結(jié)果耐藥細(xì)胞SKOV3/TAX300,耐藥指數(shù)為4.60,耐藥細(xì)胞SKOV3/TAX30,耐藥指數(shù)為51.31;與敏感細(xì)胞相比,耐藥細(xì)胞體積增大,形態(tài)不規(guī)則,倍增時間延長。多藥耐藥基因1(MDR1)在兩種耐藥細(xì)胞中高表達(dá),肺耐藥相關(guān)蛋白(LRP)、蛋白激酶Cα(PRKCA)基因僅在SKOV3/TAX300中的表達(dá)增高,BCL-2樣1基因(BCL2L1)在SKOV3/TAX30中低表達(dá)。耐藥細(xì)胞凍存在含有藥物的條件下,有利于耐藥性的保持。結(jié)論大劑量沖擊和小劑量濃度遞增兩種方法誘導(dǎo)的耐藥細(xì)胞為研究紫杉醇耐藥機(jī)制建立了良好的體外模型,兩種耐藥細(xì)胞SKOV3/TAX300和SKOV3/TAX30具有不同的生物學(xué)特性。
[Abstract]:Objective to study the biological characteristics of paclitaxel induced ovarian cancer cell lines and to explore the long-term preservation conditions of drug resistant cells in order to establish a stable experimental model in vitro. Methods the chemotherapeutic sensitive cell line SKOV3 of ovarian cancer was selected and the drug resistant ovarian cancer cell line SKOV3/TAX30 was induced by high dose impact and low dose concentration increase respectively. 0, SKOV3/TAX30. four methyl azazolium salt (MTT) assay was used to detect the IC50 of chemotherapeutic drug paclitaxel, the formation of cell plate clone of drug resistant cells and sensitive cells, the difference of multiplication time of growth curve, the expression of drug resistance related genes in sensitive and drug-resistant cells by fluorescence quantitative PCR, and the comparison of drug free cryopreservation and drug freeze. The resistance cell SKOV3/TAX300, the resistance index was 4.60, the resistance cell SKOV3/TAX30, the resistance index was 51.31, the volume of the drug resistant cells, the irregular shape, the doubling time, and the high expression of multidrug resistance gene 1 (MDR1) in two resistant cells and lung resistance related eggs. White (LRP), protein kinase C alpha (PRKCA) gene only increased in SKOV3/TAX300, BCL-2 like 1 gene (BCL2L1) was low in SKOV3/TAX30. Drug-resistant cells were frozen in the presence of drugs and were beneficial to the retention of drug resistance. Conclusion two methods induced by large dose impact and low dose concentration were used to study taxol tolerance The drug mechanism established a good in vitro model, and the two kinds of drug-resistant cells SKOV3/TAX300 and SKOV3/TAX30 have different biological characteristics.

【作者單位】： 首都醫(yī)科大學(xué)附屬北京婦產(chǎn)醫(yī)院婦瘤科;首都醫(yī)科大學(xué)附屬北京世紀(jì)壇醫(yī)院婦產(chǎn)科;
【基金】：首都醫(yī)科大學(xué)基礎(chǔ)臨床合作研究基金(12JL64)
【分類號】：R737.31

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本文編號：1814190