本文選題：孕激素 + 重度子癇前期　； 參考：《實用婦產科雜志》2015年11期

【摘要】：目的:研究孕激素在重度子癇前期孕婦外周血血漿中的水平及其在重度子癇前期發(fā)病機制Toll樣受體4(TLR4)信號通路中的作用。方法:選擇重度子癇前期孕婦30例(重度子癇前期組)和正常孕婦30例(正常對照組),采用稀釋定量法分別測定其血漿孕激素水平;原代培養(yǎng)重度子癇前期組孕婦外周血單個核細胞(PBMC),以不同濃度(0、10-8mol/L、10-6mol/L、10-4mol/L)孕激素(孕酮)刺激,實時定量聚合酶聯反應檢測TLR4、髓樣分化因子88(My D88)、核轉錄因子-κB(NF-κB)mRNA的表達;酶聯免疫吸附試驗檢測腫瘤壞死因子-α(TNF-α)及白介素-6(IL-6)蛋白的表達。結果:重度子癇前期組孕婦血漿孕激素水平明顯低于正常對照組(P0.05)。隨著孕激素濃度的增加,重度子癇前期組孕婦TLR4、My D88、NF-κB mRNA的相對表達量逐漸減少,4組組間兩兩比較,差異均有統計學意義(P0.01);TNF-α及IL-6蛋白的表達均減少,4組組間兩兩比較,差異均有統計學意義(P0.01)。結論:孕激素水平在重度子癇前期孕婦外周血漿中明顯降低,孕激素可在重度子癇前期發(fā)病機制TLR4信號通路中發(fā)揮抑制作用,從而對機體產生保護作用。
[Abstract]:Objective: To study the role of progestin in peripheral blood plasma in pregnant women with severe preeclampsia and its role in the Toll like receptor 4 (TLR4) signaling pathway in the pathogenesis of severe preeclampsia. Methods: 30 cases of severe preeclampsia (severe preeclampsia) and 30 normal pregnant women (normal control group) were selected and measured by dilution quantitative method respectively. Plasma progesterone level; primary cultured preeclampsia pregnant women peripheral blood mononuclear cells (PBMC), with different concentrations (0,10-8mol/L, 10-6mol/L, 10-4mol/L) progesterone (progesterone) stimulation, real-time quantitative polymerase chain reaction to detect TLR4, myeloid differentiation factor 88 (My D88), nuclear factor kappa B (NF- kappa B) mRNA expression; enzyme linked immunosorbent assay The expression of tumor necrosis factor - alpha (TNF- - alpha) and interleukin -6 (IL-6) protein was detected. Results: the plasma progesterone level of pregnant women with severe preeclampsia was significantly lower than that of the normal control group (P0.05). With the increase of the concentration of progestin, the relative expression of TLR4, My D88, NF- kappa B mRNA in pregnant women with severe preeclampsia gradually decreased, and the difference between the 4 groups was 22, and the difference was poor. The difference was statistically significant (P0.01), the expression of TNF- alpha and IL-6 protein decreased, and the difference between the 4 groups was statistically significant (P0.01). Conclusion: progestin levels in the peripheral plasma of pregnant women with severe preeclampsia were significantly decreased, progestin could play an inhibitory role in the pathogenesis of severe pre eclampsia TLR4 signaling pathway. The body has a protective effect.

【作者單位】： 華中科技大學同濟醫(yī)學院附屬協和醫(yī)院;
【分類號】：R714.244
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本文編號：1802690