本文選題：光動(dòng)力療法 + 焦脫鎂葉綠酸甲酯��； 參考：《第二軍醫(yī)大學(xué)學(xué)報(bào)》2015年11期

【摘要】：目的探討焦脫鎂葉綠酸甲酯(methyl pyropheophorbide-a,Mppa)介導(dǎo)的光動(dòng)力療法(Mppa-PDT)抑制人卵巢癌SKOV3細(xì)胞活性、觸發(fā)其凋亡的機(jī)制。方法將處于對(duì)數(shù)生長期的人卵巢癌SKOV3細(xì)胞隨機(jī)分為Mppa-PDT處理組(加光敏劑Mppa和LED光照處理)和對(duì)照組(空白對(duì)照、僅加光敏劑Mppa單藥對(duì)照、僅接受LED單光對(duì)照)。Mppa-PDT作用于人卵巢癌SKOV3細(xì)胞后,CCK-8法檢測細(xì)胞活性;AnnexinⅤ-FITC/PI雙染流式細(xì)胞術(shù)檢測細(xì)胞凋亡率;DAPI染色觀察細(xì)胞凋亡的核的形態(tài)學(xué)改變;DCFH-DA染色觀察細(xì)胞內(nèi)活性氧(ROS)的產(chǎn)生;單細(xì)胞凝膠電泳觀察DNA損傷情況;蛋白質(zhì)印跡法檢測p53、Caspase-3、Bax、Bcl-2蛋白的表達(dá)變化。結(jié)果 (1)Mppa-PDT能顯著抑制人卵巢癌SKOV3細(xì)胞的活性,其抑制作用具有一定的劑量依賴性;(2)Mppa-PDT誘導(dǎo)的人卵巢癌SKOV3細(xì)胞凋亡率高于空白對(duì)照、單藥對(duì)照和單光對(duì)照組(P0.05),且空白、單藥、單光3組對(duì)照組間凋亡率差異無統(tǒng)計(jì)學(xué)意義(P0.05);(3)Mppa-PDT作用于人卵巢癌SKOV3細(xì)胞后,DAPI細(xì)胞核熒光染色可見細(xì)胞核深染的凋亡細(xì)胞;DCFH-DA熒光染色發(fā)現(xiàn)Mppa-PDT組細(xì)胞內(nèi)ROS水平高于3個(gè)對(duì)照組;單細(xì)胞凝膠電泳顯示Mppa-PDT組的DNA損傷情況高于3個(gè)對(duì)照組;蛋白質(zhì)印跡法檢測發(fā)現(xiàn)p53、Caspase-3、Bax蛋白表達(dá)升高,Bcl-2蛋白表達(dá)降低(P0.05)。結(jié)論 Mppa介導(dǎo)的光動(dòng)力療法能夠抑制人卵巢癌SKOV3細(xì)胞活性并誘發(fā)其凋亡,且伴隨有DNA損傷及線粒體凋亡途徑的激活。
[Abstract]:Objective to investigate the mechanism of methyl pyropheophorbide-a (Mppa) -mediated photodynamic therapy (Mppa-PDT) in inhibiting the activity of human ovarian cancer SKOV3 cells and triggering its apoptosis. Methods Human ovarian cancer SKOV3 cells in logarithmic growth phase were randomly divided into Mppa-PDT treatment group (treated with Guang Min Mppa and LED) and control group (blank control group, single drug control with Guang Min Mppa alone). Apoptosis rate of Human Ovarian Cancer SKOV3 cells treated only with LED single Light Control Mppa-PDT and CCK-8 Assay for Cell activity Annexin 鈪，

本文編號(hào)：1797037